Restriction map for
pF1KB7222
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccI AccII AclI AcsI
AflII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AvrII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BsaBI BsiWI BsoBI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FseI HaeII
HpaI KasI KpnI KspI MamI
MfeI MluI MseI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1153 GACGTC GACGT^C
AccIII 443 TCCGGA T^CCGGA
AcyI 1153 GACGTC G[GA]^CG[CT]C
Alw44I 1238 GTGCAC G^TGCAC
AlwNI 1141 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1160 GGGCCC GGGCC^C
ApaLI 1238 GTGCAC G^TGCAC
AviII 608 TGCGCA TGC^GCA
BalI 134 TGGCCA TGG^CCA
BamHI 614 GGATCC G^GATCC
BanI 809 GGTGCC G^G[CT][GA]CC
BclI 1073 TGATCA T^GATCA
BfaI 1301 CTAG C^TAG
BlpI 985 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 985 GCTCAGC GC^T[ACGT]AGC
BsaAI 1185 CACGTA [CT]AC^GT[GA]
BsaHI 1153 GACGTC G[GA]^CG[CT]C
BsaWI 443 TCCGGA [AT]^CCGG[AT]
BseAI 443 TCCGGA T^CCGGA
BsgI 1304 GTGCAG GTGCAG(16/14)
BsiEI 315 CGACCG CG[GA][CT]^CG
BsmI 492 GAATGC GAATGC(1/-1)
BspEI 443 TCCGGA T^CCGGA
BspLU11I 416 ACATGT A^CATGT
BsrBI 1120 GAGCGG CCGCTC(-3/-3)
BssSI 77 CTCGTG CACGAG(-5/-1)
BstEII 739 GGTGACC G^GT[ACGT]ACC
BstPI 739 GGTGACC G^GT[ACGT]ACC
BstXI 497 CCAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 985 GCTCAGC GC^T[ACGT]AGC
DrdI 561 GACGGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 30 CTCTTC CTCTTC(1/4)
EcoO65I 739 GGTGACC G^GT[ACGT]ACC
FbaI 1073 TGATCA T^GATCA
FspI 608 TGCGCA TGC^GCA
Hin1I 1153 GACGTC G[GA]^CG[CT]C
HindIII 112 AAGCTT A^AGCTT
Ksp632I 30 CTCTTC CTCTTC(1/4)
MaeI 1301 CTAG C^TAG
MluNI 134 TGGCCA TGG^CCA
MroI 443 TCCGGA T^CCGGA
MscI 134 TGGCCA TGG^CCA
NspI 416 ACATGT [GA]CATG^[CT]
PflMI 888 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 407 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 457 GAGCTC GAGCT^C
SexAI 1139 ACCAGGT A^CC[AT]GGT
SfiI 1089 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 1012 GAATC G^A[AT]TC
Tsp45I 740 GTGAC ^GT[GC]AC
Tsp509I 527 AATT ^AATT
Van91I 888 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 497 CCAGGCTGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 416 ACATGT A^C[GA][CT]GT
1184 ACACGT
AhdI 427 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
950 GACGCACGGTC
AspEI 427 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
950 GACGCACGGTC
AspHI 457 GAGCTC G[AT]GC[AT]^C
1238 GTGCAC
BsaI 295 GAGACC GGTCTC(1/5)
38 GGTCTC
BsiHKAI 457 GAGCTC G[AT]GC[AT]^C
1238 GTGCAC
BspMI 405 GCAGGT ACCTGC(4/8)
891 GCAGGT
BsrDI 88 GCAATG GCAATG(2/0)
284 GCAATG
BsrFI 22 GCCGGC [GA]^CCGG[CT]
325 GCCGGC
BstAPI 101 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1313 GCACAAACTGC
BstYI 614 GGATCC [GA]^GATC[CT]
1103 AGATCC
CfoI 609 GCGC GCG^C
726 GCGC
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
325 GCCGGC
DraII 407 AGGTCCC [GA]G^G[ACGT]CC[CT]
1193 GGGGCCT
Eam1105I 427 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
950 GACGCACGGTC
EcoO109I 407 AGGTCCC [GA]G^G[ACGT]CC[CT]
1193 GGGGCCT
HhaI 609 GCGC GCG^C
726 GCGC
HinP1I 609 GCGC G^CGC
726 GCGC
HincII 778 GTTGAC GT[CT]^[GA]AC
958 GTCAAC
HindII 778 GTTGAC GT[CT]^[GA]AC
958 GTCAAC
MaeIII 740 GTGAC ^GT[ACGT]AC
1229 GTAAC
MflI 614 GGATCC [GA]^GATC[CT]
1103 AGATCC
NaeI 22 GCCGGC GCC^GGC
325 GCCGGC
NgoMIV 22 GCCGGC G^CCGGC
325 GCCGGC
SmlI 16 CTCAAG C^T[CT][GA]AG
43 CTCAAG
TaqI 452 TCGA T^CGA
941 TCGA
TthHB8I 452 TCGA T^CGA
941 TCGA
XhoII 614 GGATCC [GA]^GATC[CT]
1103 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoNI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI