Restriction map for
pF1KB8289
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 5 ACGTGT A^C[GA][CT]GT
AlwI 146 GGATC GGATC(4/5)
AvaI 299 CTCGGG C^[CT]CG[GA]G
BalI 500 TGGCCA TGG^CCA
BanI 674 GGTGCC G^G[CT][GA]CC
BbvCI 327 CCTCAGC CCTCAGC(-5/-2)
BcnI 140 CCCGG CC^[GC]GG
BlpI 429 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 429 GCTCAGC GC^T[ACGT]AGC
BseRI 85 GAGGAG GAGGAG(10/8)
BsoBI 299 CTCGGG C^[CT]CG[GA]G
BspMI 322 ACCTGC ACCTGC(4/8)
BsrI 495 ACTGG ACTGG(1/-1)
BssSI 798 CACGAG CACGAG(-5/-1)
BstYI 146 GGATCT [GA]^GATC[CT]
CelII 429 GCTCAGC GC^T[ACGT]AGC
DpnII 147 GATC ^GATC
EcoT14I 606 CCAAGG C^C[AT][AT]GG
FauI 571 GCGGG CCCGC(4/6)
HincII 373 GTCAAC GT[CT]^[GA]AC
HindII 373 GTCAAC GT[CT]^[GA]AC
HindIII 216 AAGCTT A^AGCTT
HinfI 723 GACTC G^A[ACGT]TC
MboI 147 GATC ^GATC
MflI 146 GGATCT [GA]^GATC[CT]
MluNI 500 TGGCCA TGG^CCA
MscI 500 TGGCCA TGG^CCA
NaeI 339 GCCGGC GCC^GGC
NciI 140 CCCGG CC^[GC]GG
NdeII 147 GATC ^GATC
NgoMIV 339 GCCGGC G^CCGGC
PleI 723 GACTC GAGTC(4/5)
PstI 551 CTGCAG CTGCA^G
PvuII 548 CAGCTG CAG^CTG
Sau3AI 147 GATC ^GATC
StyI 606 CCAAGG C^C[AT][AT]GG
Tsp509I 113 AATT ^AATT
XbaI 354 TCTAGA T^CTAGA
XhoII 146 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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BglI 502 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
559 GCCGTGACGGC
BpmI 334 CTCCAG CTGGAG(16/14)
82 CTGGAG
BsrFI 55 ACCGGC [GA]^CCGG[CT]
339 GCCGGC
BstAPI 544 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
842 GCAACCACTGC
Cac8I 339 GCCGGC GC[ACGT]^[ACGT]GC
343 GCCAGC
Cfr10I 55 ACCGGC [GA]^CCGG[CT]
339 GCCGGC
DsaI 248 CCGTGG C^C[GA][CT]GG
470 CCGTGG
MaeII 5 ACGT A^CGT
487 ACGT
MslI 727 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
817 CACACAGGTG
MspA1I 548 CAGCTG C[AC]G^C[GT]G
657 CCGCTG
NspI 103 ACATGC [GA]CATG^[CT]
628 GCATGT
SapI 270 GAAGAGC GCTCTTC(1/4)
347 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BfaI BmyI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
Ksp632I MaeI MaeIII MboII MnlI
MseI MspI MvaI MwoI NlaIII
NlaIV SacI Sau96I ScrFI SfcI
Tru9I TseI Tsp45I TspRI