Restriction map for
pF1KB8431
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HindIII
HpaII KasI KpnI KspI MfeI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1215 GTCTAC GT^[AC][GT]AC
AflIII 1824 ACATGT A^C[GA][CT]GT
AseI 226 ATTAAT AT^TAAT
AsnI 226 ATTAAT AT^TAAT
AvaI 416 CTCGAG C^[CT]CG[GA]G
BbvCI 1125 GCTGAGG CCTCAGC(-5/-2)
BsaBI 1396 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 242 CTCCTC GAGGAG(10/8)
BsmAI 521 GTCTC GTCTC(1/5)
BsoBI 416 CTCGAG C^[CT]CG[GA]G
BspLU11I 1824 ACATGT A^CATGT
BstAPI 810 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 763 GGTAACC G^GT[ACGT]ACC
BstPI 763 GGTAACC G^GT[ACGT]ACC
BstYI 1431 AGATCC [GA]^GATC[CT]
EcoNI 1197 CCTTACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 763 GGTAACC G^GT[ACGT]ACC
EcoRV 1516 GATATC GAT^ATC
HaeIII 1590 GGCC GG^CC
HgaI 567 GCGTC GACGC(5/10)
HpaI 780 GTTAAC GTT^AAC
MaeII 1687 ACGT A^CGT
MamI 1396 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1431 AGATCC [GA]^GATC[CT]
MwoI 810 GCAACACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1558 CATATG CA^TATG
PaeR7I 416 CTCGAG C^TCGAG
PshBI 226 ATTAAT AT^TAAT
PstI 1341 CTGCAG CTGCA^G
SapI 187 GCTCTTC GCTCTTC(1/4)
SphI 195 GCATGC GCATG^C
SspI 99 AATATT AAT^ATT
TfiI 1401 GAATC G^A[AT]TC
TspRI 372 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 416 CTCGAG C^TCGAG
XhoII 1431 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 622 GTAC GT^AC
873 GTAC
AlwI 658 GATCC GGATC(4/5)
1432 GATCC
BbsI 136 GTCTTC GAAGAC(2/6)
1262 GTCTTC
BbvI 146 GCAGC GCAGC(8/12)
121 GCTGC
BpmI 33 CTCCAG CTGGAG(16/14)
1483 CTGGAG
BpuAI 136 GTCTTC GAAGAC(2/6)
1262 GTCTTC
BsrDI 516 GCAATG GCAATG(2/0)
1736 GCAATG
BstXI 952 CCATCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1625 CCAAAGTAATGG
Cac8I 195 GCATGC GC[ACGT]^[ACGT]GC
1535 GCAAGC
DsaI 749 CCATGG C^C[GA][CT]GG
899 CCATGG
EarI 188 CTCTTC CTCTTC(1/4)
91 GAAGAG
Fnu4HI 121 GCTGC GC^[ACGT]GC
146 GCAGC
ItaI 121 GCTGC GC^[ACGT]GC
146 GCAGC
Ksp632I 188 CTCTTC CTCTTC(1/4)
91 GAAGAG
NcoI 749 CCATGG C^CATGG
899 CCATGG
RsaI 622 GTAC GT^AC
873 GTAC
SfcI 1018 CTACAG C^T[GA][CT]AG
1341 CTGCAG
TseI 121 GCTGC G^C[AT]GC
146 GCAGC
XcmI 434 CCATAATAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1802 CCAAATCTACAATGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AluI ApoI AvaII BfaI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FokI HincII HindII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
NspI PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I Tsp45I Tsp509I TthHB8I