Restriction map for
pF1KA1250
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# NO CUT ENZYMES ( 59 enzymes) #
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AatII Acc65I AgeI AhdI AocI
AscI Asp718I AspEI BclI BsaI
BsiEI BsiWI BsrFI BssHII BssSI
Bst1107I BstZ17I Bsu36I Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoT22I
FbaI FseI KpnI KspI MfeI
MluI MunI NaeI NgoMIV NotI
NruI NsiI PinAI PmeI PstI
PvuI PvuII RsrII SacII SalI
SbfI SexAI SfiI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AccI 4206 GTCTAC GT^[AC][GT]AC
AccII 3919 CGCG CG^CG
AccIII 5247 TCCGGA T^CCGGA
AclI 5213 AACGTT AA^CGTT
AflII 4525 CTTAAG C^TTAAG
AflIII 1960 ACATGT A^C[GA][CT]GT
Alw44I 250 GTGCAC G^TGCAC
Aor51HI 858 AGCGCT AGC^GCT
ApaLI 250 GTGCAC G^TGCAC
AseI 168 ATTAAT AT^TAAT
AsnI 168 ATTAAT AT^TAAT
AviII 2501 TGCGCA TGC^GCA
AvrII 3265 CCTAGG C^CTAGG
BamHI 4419 GGATCC G^GATCC
BbeI 3270 GGCGCC GGCGC^C
BbrPI 3844 CACGTG CAC^GTG
BcgI 1855 GCAACCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4525 CTTAAG C^TTAAG
BglII 4225 AGATCT A^GATCT
BlnI 3265 CCTAGG C^CTAGG
BlpI 4678 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4678 GCTGAGC GC^T[ACGT]AGC
BsaAI 3844 CACGTG [CT]AC^GT[GA]
BsaBI 583 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5247 TCCGGA T^CCGGA
BsmBI 2588 GAGACG CGTCTC(1/5)
Bsp1407I 404 TGTACA T^GTACA
BspEI 5247 TCCGGA T^CCGGA
BspHI 2222 TCATGA T^CATGA
BspLU11I 1960 ACATGT A^CATGT
BspMI 3334 GCAGGT ACCTGC(4/8)
BsrBI 3241 CCGCTC CCGCTC(-3/-3)
BsrGI 404 TGTACA T^GTACA
BstAPI 756 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3988 TTCGAA TT^CGAA
BstEII 2775 GGTTACC G^GT[ACGT]ACC
BstPI 2775 GGTTACC G^GT[ACGT]ACC
BstUI 3919 CGCG CG^CG
CelII 4678 GCTGAGC GC^T[ACGT]AGC
DsaI 572 CCATGG C^C[GA][CT]GG
Eco47III 858 AGCGCT AGC^GCT
EcoO65I 2775 GGTTACC G^GT[ACGT]ACC
FspI 2501 TGCGCA TGC^GCA
HpaI 283 GTTAAC GTT^AAC
KasI 3270 GGCGCC G^GCGCC
MamI 583 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5247 TCCGGA T^CCGGA
MvnI 3919 CGCG CG^CG
NarI 3270 GGCGCC GG^CGCC
NcoI 572 CCATGG C^CATGG
NdeI 779 CATATG CA^TATG
NheI 2896 GCTAGC G^CTAGC
NspV 3988 TTCGAA TT^CGAA
PacI 169 TTAATTAA TTAAT^TAA
PflMI 1564 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3844 CACGTG CAC^GTG
PmlI 3844 CACGTG CAC^GTG
PshBI 168 ATTAAT AT^TAAT
Psp1406I 5213 AACGTT AA^CGTT
RcaI 2222 TCATGA T^CATGA
ScaI 3816 AGTACT AGT^ACT
SfoI 3270 GGCGCC GGC^GCC
SfuI 3988 TTCGAA TT^CGAA
SmaI 4201 CCCGGG CCC^GGG
SphI 5005 GCATGC GCATG^C
SspBI 404 TGTACA T^GTACA
StuI 5140 AGGCCT AGG^CCT
Van91I 1564 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1560 CCACCCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4201 CCCGGG C^CCGGG
XmaI 4201 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 3270 GGCGCC G[GA]^CG[CT]C
3963 GACGCC
AlwNI 757 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
2356 CAGATGCTG
AspI 2344 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACACTGTC
BciVI 807 GGATAC GTATCC(6/5)
2615 GGATAC
BcnI 4201 CCCGG CC^[GC]GG
4202 CCGGG
BglI 2257 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4892 GCCTGAGTGGC
BsaHI 3270 GGCGCC G[GA]^CG[CT]C
3963 GACGCC
BsaWI 2930 ACCGGA [AT]^CCGG[AT]
5247 TCCGGA
BseRI 1717 CTCCTC GAGGAG(10/8)
3576 CTCCTC
BsmI 4963 GAATGC GAATGC(1/-1)
5306 GCATTC
BspDI 2317 ATCGAT AT^CGAT
4372 ATCGAT
BstXI 418 CCAATCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1376 CCATTTGTGTGG
ClaI 2317 ATCGAT AT^CGAT
4372 ATCGAT
EcoRI 2157 GAATTC G^AATTC
3621 GAATTC
EcoRV 12 GATATC GAT^ATC
958 GATATC
Hin1I 3270 GGCGCC G[GA]^CG[CT]C
3963 GACGCC
MspA1I 3375 CAGCGG C[AC]G^C[GT]G
4866 CAGCGG
NciI 4201 CCCGG CC^[GC]GG
4202 CCGGG
PaeR7I 3122 CTCGAG C^TCGAG
3579 CTCGAG
PflFI 2344 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACACTGTC
PshAI 1277 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2797 GACATCAGTC
SacI 3949 GAGCTC GAGCT^C
5251 GAGCTC
Tth111I 2344 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACACTGTC
XhoI 3122 CTCGAG C^TCGAG
3579 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI