Restriction map for
pF1KA0494
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspI AviII BamHI
BbeI BbrPI BbsI BbvCI BcnI
BfrI BglI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I FauI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 848 GGTACC G^GTACC
AhdI 118 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 116 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 848 GGTACC G^GTACC
AspEI 118 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 874 CTCGAG C^[CT]CG[GA]G
AvaII 1379 GGACC G^G[AT]CC
AvrII 1386 CCTAGG C^CTAGG
BalI 1338 TGGCCA TGG^CCA
BanI 848 GGTACC G^G[CT][GA]CC
BanII 16 GAGCTC G[GA]GC[CT]^C
BcgI 933 CGATGTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1099 GTATCC GTATCC(6/5)
BclI 1127 TGATCA T^GATCA
BlnI 1386 CCTAGG C^CTAGG
BseRI 823 GAGGAG GAGGAG(10/8)
BsoBI 874 CTCGAG C^[CT]CG[GA]G
BspMI 282 GCAGGT ACCTGC(4/8)
BsrBI 220 CCGCTC CCGCTC(-3/-3)
BsrDI 1220 CATTGC GCAATG(2/0)
BstBI 326 TTCGAA TT^CGAA
CfoI 9 GCGC GCG^C
DraI 603 TTTAAA TTT^AAA
EaeI 1338 TGGCCA [CT]^GGCC[GA]
Eam1105I 118 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 148 GAATTC G^AATTC
EcoRV 796 GATATC GAT^ATC
EcoT22I 23 ATGCAT ATGCA^T
FbaI 1127 TGATCA T^GATCA
HapII 51 CCGG C^CGG
HgaI 611 GCGTC GACGC(5/10)
HhaI 9 GCGC GCG^C
HinP1I 9 GCGC G^CGC
HpaII 51 CCGG C^CGG
KpnI 848 GGTACC GGTAC^C
MaeII 1346 ACGT A^CGT
MluNI 1338 TGGCCA TGG^CCA
MscI 1338 TGGCCA TGG^CCA
MslI 462 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 51 CCGG C^CGG
NlaIII 984 CATG CATG^
NlaIV 848 GGTACC GG[ACGT]^[ACGT]CC
NsiI 23 ATGCAT ATGCA^T
NspV 326 TTCGAA TT^CGAA
PaeR7I 874 CTCGAG C^TCGAG
PflMI 1341 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 16 GAGCTC GAGCT^C
SapI 1183 GAAGAGC GCTCTTC(1/4)
SfuI 326 TTCGAA TT^CGAA
StuI 1162 AGGCCT AGG^CCT
Van91I 1341 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1341 CCAGGACGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 874 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 804 GTAC GT^AC
849 GTAC
AlwI 300 GGATC GGATC(4/5)
1026 GGATC
Asp700I 319 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1309 GAAGATCTTC
AspHI 16 GAGCTC G[AT]GC[AT]^C
1133 GTGCTC
BsaBI 453 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
685 GATATGAATC
BsaI 1171 GAGACC GGTCTC(1/5)
1247 GAGACC
BsaJI 182 CCAAGG C^C[ACGT][ACGT]GG
1386 CCTAGG
BsiHKAI 16 GAGCTC G[AT]GC[AT]^C
1133 GTGCTC
BsmFI 44 GGGAC GGGAC(10/14)
567 GGGAC
BsrI 1336 ACTGG ACTGG(1/-1)
1252 CCAGT
Cfr13I 73 GGCCC G^G[ACGT]CC
1379 GGACC
EcoT14I 182 CCAAGG C^C[AT][AT]GG
1386 CCTAGG
HincII 489 GTTGAC GT[CT]^[GA]AC
916 GTCAAC
HindII 489 GTTGAC GT[CT]^[GA]AC
916 GTCAAC
MamI 453 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
685 GATATGAATC
MspA1I 530 CAGCTG C[AC]G^C[GT]G
1123 CAGCTG
PvuII 530 CAGCTG CAG^CTG
1123 CAGCTG
RsaI 804 GTAC GT^AC
849 GTAC
Sau96I 73 GGCCC G^G[ACGT]CC
1379 GGACC
StyI 182 CCAAGG C^C[AT][AT]GG
1386 CCTAGG
TaqI 327 TCGA T^CGA
875 TCGA
TthHB8I 327 TCGA T^CGA
875 TCGA
XmnI 319 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1309 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BglII BmyI BpmI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII PleI
PstI Sau3AI ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII