Restriction map for
pF1KE0173
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BbrPI BbsI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeIII MamI MfeI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI TaqI Tru9I Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 43 GACGTC GACGT^C
AfaI 66 GTAC GT^AC
AflIII 125 ACGCGT A^C[GA][CT]GT
AlwNI 13 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 70 CCTAAGG CC^T[ACGT]AGG
ApaI 699 GGGCCC GGGCC^C
AspHI 532 GAGCTC G[AT]GC[AT]^C
AvaII 243 GGACC G^G[AT]CC
BamHI 404 GGATCC G^GATCC
BbeI 685 GGCGCC GGCGC^C
BbvCI 676 GCTGAGG CCTCAGC(-5/-2)
BciVI 753 GGATAC GTATCC(6/5)
BcnI 498 CCGGG CC^[GC]GG
BfaI 31 CTAG C^TAG
BsiHKAI 532 GAGCTC G[AT]GC[AT]^C
BsmBI 557 GAGACG CGTCTC(1/5)
BspMI 435 GCAGGT ACCTGC(4/8)
BsrFI 853 GCCGGC [GA]^CCGG[CT]
BssSI 524 CACGAG CACGAG(-5/-1)
Bsu36I 70 CCTAAGG CC^T[ACGT]AGG
Cfr10I 853 GCCGGC [GA]^CCGG[CT]
DraII 699 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 70 CCTAAGG CC^T[ACGT]AGG
EcoNI 801 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 699 GGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 685 GGCGCC [GA]GCGC^[CT]
HphI 438 GGTGA GGTGA(8/7)
KasI 685 GGCGCC G^GCGCC
MaeI 31 CTAG C^TAG
MluI 125 ACGCGT A^CGCGT
MslI 721 CACTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 853 GCCGGC GCC^GGC
NarI 685 GGCGCC GG^CGCC
NciI 498 CCGGG CC^[GC]GG
NgoMIV 853 GCCGGC G^CCGGC
PleI 79 GACTC GAGTC(4/5)
PvuII 541 CAGCTG CAG^CTG
RsaI 66 GTAC GT^AC
SacI 532 GAGCTC GAGCT^C
SapI 534 GCTCTTC GCTCTTC(1/4)
SbfI 870 CCTGCAGG CCTGCA^GG
SfoI 685 GGCGCC GGC^GCC
SmlI 621 CTTGAG C^T[CT][GA]AG
Sse8387I 870 CCTGCAGG CCTGCA^GG
TfiI 836 GATTC G^A[AT]TC
Tsp509I 713 AATT ^AATT
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BalI 195 TGGCCA TGG^CCA
584 TGGCCA
BanI 685 GGCGCC G^G[CT][GA]CC
842 GGTGCC
BglI 701 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
869 GCCTGCAGGGC
BpmI 482 CTCCAG CTGGAG(16/14)
767 CTGGAG
BsgI 138 CTGCAC GTGCAG(16/14)
820 GTGCAG
BsmAI 557 GAGAC GTCTC(1/5)
770 GAGAC
BsmFI 99 GTCCC GGGAC(10/14)
280 GTCCC
BstXI 540 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
587 CCAAGCACTTGG
CfoI 114 GCGC GCG^C
686 GCGC
DsaI 290 CCACGG C^C[GA][CT]GG
506 CCGTGG
EarI 535 CTCTTC CTCTTC(1/4)
4 GAAGAG
FauI 735 CCCGC CCCGC(4/6)
743 CCCGC
HhaI 114 GCGC GCG^C
686 GCGC
HinP1I 114 GCGC G^CGC
686 GCGC
HinfI 79 GACTC G^A[ACGT]TC
836 GATTC
Ksp632I 535 CTCTTC CTCTTC(1/4)
4 GAAGAG
MaeII 44 ACGT A^CGT
560 ACGT
MluNI 195 TGGCCA TGG^CCA
584 TGGCCA
MscI 195 TGGCCA TGG^CCA
584 TGGCCA
MspA1I 451 CAGCGG C[AC]G^C[GT]G
541 CAGCTG
NlaIII 177 CATG CATG^
199 CATG
PstI 448 CTGCAG CTGCA^G
871 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BanII BbvI BmyI BsaHI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI Hin1I HpaII ItaI MboI
MboII MflI MnlI MspI MvaI
MvnI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI XhoII