Restriction map for
pF1KE0529
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BfaI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MaeI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 607 CTTAAG C^TTAAG
AflIII 5 ACGTGT A^C[GA][CT]GT
AluI 401 AGCT AG^CT
AlwNI 109 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 3 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 41 TGGCCA TGG^CCA
BclI 410 TGATCA T^GATCA
BcnI 474 CCGGG CC^[GC]GG
BfrI 607 CTTAAG C^TTAAG
BglII 65 AGATCT A^GATCT
BmyI 476 GGGCAC G[AGT]GC[ACT]^C
BsaI 433 GAGACC GGTCTC(1/5)
BsaJI 18 CCTCGG C^C[ACGT][ACGT]GG
BseRI 202 CTCCTC GAGGAG(10/8)
BsmAI 433 GAGAC GTCTC(1/5)
BsmFI 298 GTCCC GGGAC(10/14)
Bsp1286I 476 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 224 TGTACA T^GTACA
BspHI 407 TCATGA T^CATGA
BsrGI 224 TGTACA T^GTACA
BstXI 137 CCAGGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 41 TGGCCA [CT]^GGCC[GA]
FbaI 410 TGATCA T^GATCA
HapII 474 CCGG C^CGG
HpaII 474 CCGG C^CGG
MaeII 5 ACGT A^CGT
MluNI 41 TGGCCA TGG^CCA
MscI 41 TGGCCA TGG^CCA
MslI 180 CACAGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 474 CCGG C^CGG
NciI 474 CCGGG CC^[GC]GG
PshAI 597 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 407 TCATGA T^CATGA
SexAI 136 ACCAGGT A^CC[AT]GGT
SfaNI 92 GCATC GCATC(5/9)
SfcI 330 CTACAG C^T[GA][CT]AG
SmlI 607 CTTAAG C^T[CT][GA]AG
SspBI 224 TGTACA T^GTACA
XcmI 134 CCACCAGGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 140 GGTACC G^GTACC
506 GGTACC
AciI 279 CCGC CCGC(-3/-1)
504 GCGG
AcsI 241 AAATTC [GA]^AATT[CT]
519 GAATTT
ApoI 241 AAATTC [GA]^AATT[CT]
519 GAATTT
Asp718I 140 GGTACC G^GTACC
506 GGTACC
BanI 140 GGTACC G^G[CT][GA]CC
506 GGTACC
BbsI 127 GTCTTC GAAGAC(2/6)
626 GTCTTC
BbvI 54 GCTGC GCAGC(8/12)
83 GCTGC
BpuAI 127 GTCTTC GAAGAC(2/6)
626 GTCTTC
BsiYI 33 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
301 CCAGTCATTGG
BslI 33 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
301 CCAGTCATTGG
EarI 403 CTCTTC CTCTTC(1/4)
102 GAAGAG
Fnu4HI 54 GCTGC GC^[ACGT]GC
83 GCTGC
HaeIII 42 GGCC GG^CC
310 GGCC
HinfI 14 GAATC G^A[ACGT]TC
379 GAATC
HphI 131 TCACC GGTGA(8/7)
155 TCACC
ItaI 54 GCTGC GC^[ACGT]GC
83 GCTGC
KpnI 140 GGTACC GGTAC^C
506 GGTACC
Ksp632I 403 CTCTTC CTCTTC(1/4)
102 GAAGAG
MseI 608 TTAA T^TAA
622 TTAA
MwoI 23 GCGACCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
83 GCTGCAAGGGC
PflMI 33 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
301 CCAGTCATTGG
SapI 102 GAAGAGC GCTCTTC(1/4)
402 GCTCTTC
TfiI 14 GAATC G^A[AT]TC
379 GAATC
Tru9I 608 TTAA T^TAA
622 TTAA
TseI 54 GCTGC G^C[AT]GC
83 GCTGC
Tsp45I 575 GTGAC ^GT[GC]AC
595 GTGAC
Van91I 33 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
301 CCAGTCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AlwI AvaII BpmI BsrI
BssKI BstNI BstYI Cfr13I DdeI
DpnII EcoRII MaeIII MboI MboII
MflI MnlI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
Tsp509I TspRI XhoII