Restriction map for
pF1KB6487
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaII AvrII
BbrPI BbvCI BcgI BciVI BclI
BfaI BlnI BlpI Bpu1102I BsaBI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 757 GAATTT [GA]^AATT[CT]
AcyI 932 GGCGCC G[GA]^CG[CT]C
AflII 577 CTTAAG C^TTAAG
ApoI 757 GAATTT [GA]^AATT[CT]
AspHI 904 GAGCAC G[AT]GC[AT]^C
AspI 426 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 167 TGCGCA TGC^GCA
BamHI 920 GGATCC G^GATCC
BanI 932 GGCGCC G^G[CT][GA]CC
BbeI 932 GGCGCC GGCGC^C
BbsI 976 GTCTTC GAAGAC(2/6)
BfrI 577 CTTAAG C^TTAAG
BglI 28 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 659 AGATCT A^GATCT
BpuAI 976 GTCTTC GAAGAC(2/6)
BsaAI 124 TACGTG [CT]AC^GT[GA]
BsaHI 932 GGCGCC G[GA]^CG[CT]C
BsaWI 260 ACCGGA [AT]^CCGG[AT]
BsiHKAI 904 GAGCAC G[AT]GC[AT]^C
BspMI 296 ACCTGC ACCTGC(4/8)
BsrBI 264 GAGCGG CCGCTC(-3/-3)
BsrI 339 ACTGG ACTGG(1/-1)
BstXI 500 CCAAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FspI 167 TGCGCA TGC^GCA
HgaI 954 GCGTC GACGC(5/10)
Hin1I 932 GGCGCC G[GA]^CG[CT]C
KasI 932 GGCGCC G^GCGCC
MseI 578 TTAA T^TAA
MslI 447 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 932 GGCGCC GG^CGCC
NcoI 452 CCATGG C^CATGG
NotI 212 GCGGCCGC GC^GGCCGC
PaeR7I 70 CTCGAG C^TCGAG
PflFI 426 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 949 GACGAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 409 CTGCAG CTGCA^G
ScaI 542 AGTACT AGT^ACT
SexAI 371 ACCTGGT A^CC[AT]GGT
SfaNI 235 GATGC GCATC(5/9)
SfcI 409 CTGCAG C^T[GA][CT]AG
SfoI 932 GGCGCC GGC^GCC
StuI 11 AGGCCT AGG^CCT
Tru9I 578 TTAA T^TAA
TspRI 317 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 426 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 70 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 379 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
481 CAGCTCCTG
ApaI 18 GGGCCC GGGCC^C
819 GGGCCC
BalI 488 TGGCCA TGG^CCA
963 TGGCCA
BanII 18 GGGCCC G[GA]GC[CT]^C
819 GGGCCC
BpmI 838 CTGGAG CTGGAG(16/14)
860 CTGGAG
BseRI 472 GAGGAG GAGGAG(10/8)
781 GAGGAG
BsiEI 213 CGGCCG CG[GA][CT]^CG
267 CGGCCG
DraII 380 AGGGCCT [GA]G^G[ACGT]CC[CT]
818 GGGGCCC
EagI 213 CGGCCG C^GGCCG
267 CGGCCG
EarI 631 CTCTTC CTCTTC(1/4)
892 GAAGAG
EclXI 213 CGGCCG C^GGCCG
267 CGGCCG
Eco52I 213 CGGCCG C^GGCCG
267 CGGCCG
EcoO109I 380 AGGGCCT [GA]G^G[ACGT]CC[CT]
818 GGGGCCC
EcoT14I 452 CCATGG C^C[AT][AT]GG
749 CCAAGG
HaeII 405 GGCGCT [GA]GCGC^[CT]
932 GGCGCC
HinfI 426 GACTC G^A[ACGT]TC
810 GACTC
Ksp632I 631 CTCTTC CTCTTC(1/4)
892 GAAGAG
KspI 32 CCGCGG CCGC^GG
879 CCGCGG
MaeII 125 ACGT A^CGT
137 ACGT
MluNI 488 TGGCCA TGG^CCA
963 TGGCCA
MscI 488 TGGCCA TGG^CCA
963 TGGCCA
PleI 426 GACTC GAGTC(4/5)
810 GACTC
PvuII 400 CAGCTG CAG^CTG
857 CAGCTG
SacII 32 CCGCGG CCGC^GG
879 CCGCGG
SmaI 467 CCCGGG CCC^GGG
815 CCCGGG
StyI 452 CCATGG C^C[AT][AT]GG
749 CCAAGG
Tsp45I 272 GTCAC ^GT[GC]AC
457 GTGAC
XmaCI 467 CCCGGG C^CCGGG
815 CCCGGG
XmaI 467 CCCGGG C^CCGGG
815 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI BbvI BcnI BmyI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DpnII DsaI EaeI EcoNI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SmlI
TaqI TseI Tsp509I TthHB8I XhoII