Restriction map for
pF1KB9729
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AocI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AvaII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MaeII
MamI MfeI MflI MluI MunI
NcoI NdeI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SapI
ScaI SfiI SfuI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 161 GTCGAC GT^[AC][GT]AC
AccIII 964 TCCGGA T^CCGGA
AcsI 316 GAATTT [GA]^AATT[CT]
AhdI 1172 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1094 GTGCAC G^TGCAC
AlwI 967 GGATC GGATC(4/5)
Aor51HI 1081 AGCGCT AGC^GCT
ApaLI 1094 GTGCAC G^TGCAC
ApoI 316 GAATTT [GA]^AATT[CT]
AspEI 1172 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 145 TGCGCA TGC^GCA
BseAI 964 TCCGGA T^CCGGA
BsgI 1303 GTGCAG GTGCAG(16/14)
BspEI 964 TCCGGA T^CCGGA
BssHII 863 GCGCGC G^CGCGC
BstF5I 344 CATCC GGATG(2/0)
EagI 227 CGGCCG C^GGCCG
Eam1105I 1172 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 781 GAAGAG CTCTTC(1/4)
EclXI 227 CGGCCG C^GGCCG
Eco47III 1081 AGCGCT AGC^GCT
Eco52I 227 CGGCCG C^GGCCG
EcoNI 567 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 344 CATCC GGATG(9/13)
FspI 145 TGCGCA TGC^GCA
HgaI 52 GCGTC GACGC(5/10)
Ksp632I 781 GAAGAG CTCTTC(1/4)
MroI 964 TCCGGA T^CCGGA
MseI 320 TTAA T^TAA
NaeI 602 GCCGGC GCC^GGC
NgoMIV 602 GCCGGC G^CCGGC
NheI 730 GCTAGC G^CTAGC
NotI 226 GCGGCCGC GC^GGCCGC
NspI 73 ACATGC [GA]CATG^[CT]
PaeR7I 896 CTCGAG C^TCGAG
SalI 161 GTCGAC G^TCGAC
SbfI 1248 CCTGCAGG CCTGCA^GG
SexAI 583 ACCTGGT A^CC[AT]GGT
SgrAI 601 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1248 CCTGCAGG CCTGCA^GG
StuI 485 AGGCCT AGG^CCT
Tru9I 320 TTAA T^TAA
XhoI 896 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 43 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
868 CAGTCACTG
AvrII 1009 CCTAGG C^CTAGG
1111 CCTAGG
BalI 545 TGGCCA TGG^CCA
824 TGGCCA
BanII 277 GAGCCC G[GA]GC[CT]^C
748 GGGCTC
BlnI 1009 CCTAGG C^CTAGG
1111 CCTAGG
BpmI 775 CTGGAG CTGGAG(16/14)
1322 CTGGAG
BsaI 466 GAGACC GGTCTC(1/5)
1055 GGTCTC
BsaWI 183 ACCGGA [AT]^CCGG[AT]
964 TCCGGA
BspMI 260 ACCTGC ACCTGC(4/8)
1333 GCAGGT
BsrBI 134 CCGCTC CCGCTC(-3/-3)
382 GAGCGG
BsrFI 439 GCCGGT [GA]^CCGG[CT]
602 GCCGGC
Cfr10I 439 GCCGGT [GA]^CCGG[CT]
602 GCCGGC
DpnII 537 GATC ^GATC
968 GATC
DsaI 30 CCGCGG C^C[GA][CT]GG
1282 CCGCGG
HincII 161 GTCGAC GT[CT]^[GA]AC
1014 GTTGAC
HindII 161 GTCGAC GT[CT]^[GA]AC
1014 GTTGAC
KspI 30 CCGCGG CCGC^GG
1282 CCGCGG
MboI 537 GATC ^GATC
968 GATC
MluNI 545 TGGCCA TGG^CCA
824 TGGCCA
MscI 545 TGGCCA TGG^CCA
824 TGGCCA
MslI 555 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
570 CACCCAGGTG
NdeII 537 GATC ^GATC
968 GATC
PflMI 10 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
42 CCAGTTTCTGG
PvuII 236 CAGCTG CAG^CTG
830 CAGCTG
SacII 30 CCGCGG CCGC^GG
1282 CCGCGG
Sau3AI 537 GATC ^GATC
968 GATC
SmaI 461 CCCGGG CCC^GGG
743 CCCGGG
TaqI 162 TCGA T^CGA
897 TCGA
Tsp45I 761 GTGAC ^GT[GC]AC
870 GTCAC
Tsp509I 317 AATT ^AATT
428 AATT
TthHB8I 162 TCGA T^CGA
897 TCGA
Van91I 10 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
42 CCAGTTTCTGG
XcmI 243 CCAGAGCTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1311 CCAGTACCCGTCTGG
XmaCI 461 CCCGGG C^CCGGG
743 CCCGGG
XmaI 461 CCCGGG C^CCGGG
743 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanI BbeI BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstAPI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EaeI EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV PleI
PstI RsaI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TfiI
TseI TspRI