Restriction map for
pF1KB8996
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AviII 320 TGCGCA TGC^GCA
BamHI 136 GGATCC G^GATCC
BbeI 397 GGCGCC GGCGC^C
BcgI 858 GCACCGCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 742 CTAG C^TAG
BlpI 1128 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1128 GCTGAGC GC^T[ACGT]AGC
BsmAI 442 GAGAC GTCTC(1/5)
BstAPI 285 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 702 CATCC GGATG(2/0)
CelII 1128 GCTGAGC GC^T[ACGT]AGC
DraIII 286 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 564 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 305 CCATGG C^C[AT][AT]GG
FokI 702 CATCC GGATG(9/13)
FseI 268 GGCCGGCC GGCCGG^CC
FspI 320 TGCGCA TGC^GCA
HgaI 102 GACGC GACGC(5/10)
KasI 397 GGCGCC G^GCGCC
MaeI 742 CTAG C^TAG
NarI 397 GGCGCC GG^CGCC
NcoI 305 CCATGG C^CATGG
NspI 1076 GCATGC [GA]CATG^[CT]
PpuMI 1025 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 256 CTGCAG CTGCA^G
SacI 196 GAGCTC GAGCT^C
SfaNI 261 GATGC GCATC(5/9)
SfcI 256 CTGCAG C^T[GA][CT]AG
SfiI 699 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 397 GGCGCC GGC^GCC
SphI 1076 GCATGC GCATG^C
StyI 305 CCATGG C^C[AT][AT]GG
TspRI 751 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 1190 CCAACATGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 102 GACGCC G[GA]^CG[CT]C
397 GGCGCC
AocI 70 CCTGAGG CC^T[ACGT]AGG
295 CCTAAGG
AspHI 196 GAGCTC G[AT]GC[AT]^C
1131 GAGCAC
BanII 81 GGGCTC G[GA]GC[CT]^C
196 GAGCTC
BsaHI 102 GACGCC G[GA]^CG[CT]C
397 GGCGCC
BsiHKAI 196 GAGCTC G[AT]GC[AT]^C
1131 GAGCAC
BsmFI 1181 GGGAC GGGAC(10/14)
1027 GTCCC
BsmI 863 GCATTC GAATGC(1/-1)
1115 GCATTC
BsrBI 718 GAGCGG CCGCTC(-3/-3)
727 GAGCGG
BsrI 956 ACTGG ACTGG(1/-1)
1068 CCAGT
BstYI 136 GGATCC [GA]^GATC[CT]
365 AGATCC
Bsu36I 70 CCTGAGG CC^T[ACGT]AGG
295 CCTAAGG
DraII 66 GGGGCCT [GA]G^G[ACGT]CC[CT]
1025 GGGTCCC
EarI 648 GAAGAG CTCTTC(1/4)
805 GAAGAG
Eco81I 70 CCTGAGG CC^T[ACGT]AGG
295 CCTAAGG
EcoO109I 66 GGGGCCT [GA]G^G[ACGT]CC[CT]
1025 GGGTCCC
HaeII 21 GGCGCT [GA]GCGC^[CT]
397 GGCGCC
Hin1I 102 GACGCC G[GA]^CG[CT]C
397 GGCGCC
HinfI 734 GACTC G^A[ACGT]TC
808 GAGTC
Ksp632I 648 GAAGAG CTCTTC(1/4)
805 GAAGAG
MflI 136 GGATCC [GA]^GATC[CT]
365 AGATCC
PflMI 527 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
765 CCAGGAGCTGG
PleI 734 GACTC GAGTC(4/5)
808 GAGTC
PvuII 759 CAGCTG CAG^CTG
1002 CAGCTG
SmlI 246 CTTGAG C^T[CT][GA]AG
661 CTCAAG
TaqI 377 TCGA T^CGA
1154 TCGA
TthHB8I 377 TCGA T^CGA
1154 TCGA
Van91I 527 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
765 CCAGGAGCTGG
XhoII 136 GGATCC [GA]^GATC[CT]
365 AGATCC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsoBI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SmaI StuI
TseI Tsp45I XmaCI XmaI