Restriction map for
pF1KB8928
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MluI
MluNI MroI MscI MslI MvaI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AclI 174 AACGTT AA^CGTT
AflIII 134 ACGTGT A^C[GA][CT]GT
AlwNI 19 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 271 ATTAAT AT^TAAT
AsnI 271 ATTAAT AT^TAAT
Asp700I 706 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 263 GAGCAC G[AT]GC[AT]^C
AvaII 467 GGACC G^G[AT]CC
BclI 522 TGATCA T^GATCA
BcnI 50 CCGGG CC^[GC]GG
BglII 439 AGATCT A^GATCT
BmyI 263 GAGCAC G[AGT]GC[ACT]^C
BpmI 256 CTCCAG CTGGAG(16/14)
BsaAI 79 TACGTG [CT]AC^GT[GA]
BsiHKAI 263 GAGCAC G[AT]GC[AT]^C
Bsp1286I 263 GAGCAC G[AGT]GC[ACT]^C
BspMI 73 ACCTGC ACCTGC(4/8)
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BsrFI 62 GCCGGC [GA]^CCGG[CT]
BsrI 470 CCAGT ACTGG(1/-1)
BssKI 50 CCGGG ^CC[ACGT]GG
BstF5I 679 GGATG GGATG(2/0)
BstXI 700 CCAATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 40 GCGC GCG^C
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
Cfr13I 467 GGACC G^G[ACGT]CC
DdeI 402 CTCAG C^T[ACGT]AG
DraI 225 TTTAAA TTT^AAA
EcoT14I 575 CCAAGG C^C[AT][AT]GG
FauI 455 CCCGC CCCGC(4/6)
FbaI 522 TGATCA T^GATCA
FokI 679 GGATG GGATG(9/13)
HaeIII 579 GGCC GG^CC
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HindIII 596 AAGCTT A^AGCTT
MfeI 701 CAATTG C^AATTG
MspA1I 463 CAGCGG C[AC]G^C[GT]G
MunI 701 CAATTG C^AATTG
NaeI 62 GCCGGC GCC^GGC
NciI 50 CCGGG CC^[GC]GG
NgoMIV 62 GCCGGC G^CCGGC
NlaIV 59 GGAGCC GG[ACGT]^[ACGT]CC
NspI 122 ACATGC [GA]CATG^[CT]
PleI 106 GAGTC GAGTC(4/5)
PshBI 271 ATTAAT AT^TAAT
Psp1406I 174 AACGTT AA^CGTT
Sau96I 467 GGACC G^G[ACGT]CC
ScaI 805 AGTACT AGT^ACT
ScrFI 50 CCGGG CC^[ACGT]GG
SfcI 244 CTACAG C^T[GA][CT]AG
SmlI 94 CTTGAG C^T[CT][GA]AG
StyI 575 CCAAGG C^C[AT][AT]GG
TfiI 421 GATTC G^A[AT]TC
XmnI 706 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 196 GTCTAC GT^[AC][GT]AC
242 GTCTAC
AfaI 27 GTAC GT^AC
806 GTAC
AlwI 379 GATCC GGATC(4/5)
622 GATCC
BbsI 449 GAAGAC GAAGAC(2/6)
511 GAAGAC
BpuAI 449 GAAGAC GAAGAC(2/6)
511 GAAGAC
BsaJI 50 CCGGGG C^C[ACGT][ACGT]GG
575 CCAAGG
BsgI 82 GTGCAG GTGCAG(16/14)
771 GTGCAG
BsiYI 45 CCTGACCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
50 CCGGGGAGCGG
BslI 45 CCTGACCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
50 CCGGGGAGCGG
Cac8I 62 GCCGGC GC[ACGT]^[ACGT]GC
458 GCAGGC
HapII 50 CCGG C^CGG
63 CCGG
HinfI 106 GAGTC G^A[ACGT]TC
421 GATTC
HpaII 50 CCGG C^CGG
63 CCGG
HphI 71 TCACC GGTGA(8/7)
601 TCACC
MspI 50 CCGG C^CGG
63 CCGG
RsaI 27 GTAC GT^AC
806 GTAC
TaqI 323 TCGA T^CGA
835 TCGA
Tsp45I 491 GTCAC ^GT[GC]AC
683 GTGAC
TthHB8I 323 TCGA T^CGA
835 TCGA
XcmI 654 CCAAGTGTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
745 CCAAAGGGGAAATGG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BstYI DpnII
Fnu4HI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII Sau3AI Tru9I TseI
Tsp509I TspRI XhoII