Restriction map for
pF1KE0156
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BalI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI Bsp1407I BspDI BspEI
BspHI BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
AfaI 85 GTAC GT^AC
AocI 688 CCTGAGG CC^T[ACGT]AGG
AspI 361 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 572 GGATCC G^GATCC
BciVI 736 GGATAC GTATCC(6/5)
BfaI 397 CTAG C^TAG
BglI 211 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 387 GGGCAC G[AGT]GC[ACT]^C
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsiEI 69 CGGCCG CG[GA][CT]^CG
BsmI 712 GCATTC GAATGC(1/-1)
Bsp1286I 387 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 341 ACATGT A^CATGT
BsrDI 603 CATTGC GCAATG(2/0)
BstYI 572 GGATCC [GA]^GATC[CT]
Bsu36I 688 CCTGAGG CC^T[ACGT]AGG
EagI 69 CGGCCG C^GGCCG
EclXI 69 CGGCCG C^GGCCG
Eco52I 69 CGGCCG C^GGCCG
Eco81I 688 CCTGAGG CC^T[ACGT]AGG
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HincII 376 GTCAAC GT[CT]^[GA]AC
HindII 376 GTCAAC GT[CT]^[GA]AC
HinfI 23 GACTC G^A[ACGT]TC
HphI 317 TCACC GGTGA(8/7)
MaeI 397 CTAG C^TAG
MboII 727 GAAGA GAAGA(8/7)
MflI 572 GGATCC [GA]^GATC[CT]
NspI 341 ACATGT [GA]CATG^[CT]
PflFI 361 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 23 GACTC GAGTC(4/5)
PstI 410 CTGCAG CTGCA^G
RsaI 85 GTAC GT^AC
SfcI 410 CTGCAG C^T[GA][CT]AG
SmaI 551 CCCGGG CCC^GGG
SmlI 382 CTCAAG C^T[CT][GA]AG
StuI 269 AGGCCT AGG^CCT
Tth111I 361 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 245 CCAGGTGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 572 GGATCC [GA]^GATC[CT]
XmaCI 551 CCCGGG C^CCGGG
XmaI 551 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 224 ACGTGT A^C[GA][CT]GT
341 ACATGT
AvaI 194 CCCGAG C^[CT]CG[GA]G
551 CCCGGG
BanI 248 GGTGCC G^G[CT][GA]CC
388 GGCACC
BsgI 337 CTGCAC GTGCAG(16/14)
265 GTGCAG
BsmAI 148 GAGAC GTCTC(1/5)
6 GTCTC
BsmBI 5 CGTCTC CGTCTC(1/5)
148 GAGACG
BsoBI 194 CCCGAG C^[CT]CG[GA]G
551 CCCGGG
DpnII 573 GATC ^GATC
663 GATC
DraII 683 GGGACCC [GA]G^G[ACGT]CC[CT]
748 GGGACCT
EaeI 69 CGGCCG [CT]^GGCC[GA]
92 TGGCCG
EcoO109I 683 GGGACCC [GA]G^G[ACGT]CC[CT]
748 GGGACCT
EcoT14I 41 CCTTGG C^C[AT][AT]GG
515 CCAAGG
MaeII 224 ACGT A^CGT
488 ACGT
MboI 573 GATC ^GATC
663 GATC
MspA1I 137 CAGCGG C[AC]G^C[GT]G
576 CCGCTG
NdeII 573 GATC ^GATC
663 GATC
PpuMI 683 GGGACCC [GA]G^G[AT]CC[CT]
748 GGGACCT
Sau3AI 573 GATC ^GATC
663 GATC
StyI 41 CCTTGG C^C[AT][AT]GG
515 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmFI BsrBI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI MaeIII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI