Restriction map for
pF1KE0110
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BpmI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AlwI 325 GATCC GGATC(4/5)
AocI 586 CCTGAGG CC^T[ACGT]AGG
AseI 706 ATTAAT AT^TAAT
AsnI 706 ATTAAT AT^TAAT
AspHI 410 GAGCAC G[AT]GC[AT]^C
BanI 351 GGCACC G^G[CT][GA]CC
BanII 635 GGGCTC G[GA]GC[CT]^C
BbsI 543 GAAGAC GAAGAC(2/6)
BglII 36 AGATCT A^GATCT
BlpI 461 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 461 GCTTAGC GC^T[ACGT]AGC
BpuAI 543 GAAGAC GAAGAC(2/6)
BsaAI 104 TACGTG [CT]AC^GT[GA]
BsaBI 420 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 392 TCCGGT [AT]^CCGG[AT]
BseRI 589 GAGGAG GAGGAG(10/8)
BsiHKAI 410 GAGCAC G[AT]GC[AT]^C
BsmI 388 GCATTC GAATGC(1/-1)
BspHI 599 TCATGA T^CATGA
BsrBI 250 GAGCGG CCGCTC(-3/-3)
BsrDI 563 CATTGC GCAATG(2/0)
BstAPI 7 GCATGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 521 CCACAGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 36 AGATCT [GA]^GATC[CT]
Bsu36I 586 CCTGAGG CC^T[ACGT]AGG
CelII 461 GCTTAGC GC^T[ACGT]AGC
CfoI 80 GCGC GCG^C
DraII 471 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 586 CCTGAGG CC^T[ACGT]AGG
EcoO109I 471 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 196 GATATC GAT^ATC
FauI 567 GCGGG CCCGC(4/6)
HaeIII 473 GGCC GG^CC
HapII 393 CCGG C^CGG
HhaI 80 GCGC GCG^C
HinP1I 80 GCGC G^CGC
HinfI 381 GAATC G^A[ACGT]TC
HpaII 393 CCGG C^CGG
MamI 420 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 36 AGATCT [GA]^GATC[CT]
MslI 522 CACAGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 68 CAGCTG C[AC]G^C[GT]G
MspI 393 CCGG C^CGG
PshBI 706 ATTAAT AT^TAAT
PvuII 68 CAGCTG CAG^CTG
RcaI 599 TCATGA T^CATGA
SmlI 638 CTCAAG C^T[CT][GA]AG
SspI 213 AATATT AAT^ATT
TaqI 684 TCGA T^CGA
TfiI 381 GAATC G^A[AT]TC
TthHB8I 684 TCGA T^CGA
XhoII 36 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 252 GCGG CCGC(-3/-1)
567 GCGG
AcsI 190 GAATTT [GA]^AATT[CT]
374 AAATTC
ApoI 190 GAATTT [GA]^AATT[CT]
374 AAATTC
Asp700I 358 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
543 GAAGACGTTC
AvaII 478 GGACC G^G[AT]CC
753 GGACC
BfaI 247 CTAG C^TAG
277 CTAG
BsaJI 467 CCCTGG C^C[ACGT][ACGT]GG
468 CCTGGG
BsrI 574 ACTGG ACTGG(1/-1)
673 ACTGG
BstF5I 405 GGATG GGATG(2/0)
621 GGATG
FokI 405 GGATG GGATG(9/13)
621 GGATG
MaeI 247 CTAG C^TAG
277 CTAG
MaeII 105 ACGT A^CGT
547 ACGT
NlaIV 351 GGCACC GG[ACGT]^[ACGT]CC
471 GGGGCC
NspI 7 GCATGC [GA]CATG^[CT]
650 GCATGC
PstI 230 CTGCAG CTGCA^G
763 CTGCAG
SfcI 230 CTGCAG C^T[GA][CT]AG
763 CTGCAG
SphI 7 GCATGC GCATG^C
650 GCATGC
Tsp45I 133 GTGAC ^GT[GC]AC
487 GTGAC
TspRI 42 CAGTG CA[GC]TG[ACGT][ACGT]^
573 CACTG
XmnI 358 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
543 GAAGACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbvI BmyI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp509I