Restriction map for
pF1KE0470
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI Ksp632I
KspI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 80 GGTACC G^GTACC
AcsI 898 AAATTC [GA]^AATT[CT]
AflIII 764 ACATGT A^C[GA][CT]GT
AlwI 413 GGATC GGATC(4/5)
AlwNI 797 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 898 AAATTC [GA]^AATT[CT]
Asp700I 699 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 80 GGTACC G^GTACC
AvaII 641 GGACC G^G[AT]CC
BalI 224 TGGCCA TGG^CCA
BfaI 149 CTAG C^TAG
BglII 659 AGATCT A^GATCT
BlpI 345 GCTGAGC GC^T[ACGT]AGC
BmyI 574 GTGCCC G[AGT]GC[ACT]^C
BpmI 733 CTGGAG CTGGAG(16/14)
Bpu1102I 345 GCTGAGC GC^T[ACGT]AGC
BseRI 200 GAGGAG GAGGAG(10/8)
BsiYI 130 CCAACCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 130 CCAACCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 574 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 764 ACATGT A^CATGT
BspMI 77 GCAGGT ACCTGC(4/8)
BsrDI 816 CATTGC GCAATG(2/0)
BstXI 644 CCACGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 659 AGATCT [GA]^GATC[CT]
CelII 345 GCTGAGC GC^T[ACGT]AGC
Cfr13I 641 GGACC G^G[ACGT]CC
DraI 475 TTTAAA TTT^AAA
EcoRV 670 GATATC GAT^ATC
EcoT14I 694 CCTTGG C^C[AT][AT]GG
EcoT22I 498 ATGCAT ATGCA^T
HgaI 391 GCGTC GACGC(5/10)
HincII 603 GTCAAC GT[CT]^[GA]AC
HindII 603 GTCAAC GT[CT]^[GA]AC
KpnI 80 GGTACC GGTAC^C
MaeI 149 CTAG C^TAG
MflI 659 AGATCT [GA]^GATC[CT]
MluNI 224 TGGCCA TGG^CCA
MscI 224 TGGCCA TGG^CCA
MspA1I 569 CAGCGG C[AC]G^C[GT]G
NdeI 597 CATATG CA^TATG
NheI 148 GCTAGC G^CTAGC
NsiI 498 ATGCAT ATGCA^T
PstI 8 CTGCAG CTGCA^G
Sau96I 641 GGACC G^G[ACGT]CC
SfcI 8 CTGCAG C^T[GA][CT]AG
StyI 694 CCTTGG C^C[AT][AT]GG
TfiI 300 GATTC G^A[AT]TC
XhoII 659 AGATCT [GA]^GATC[CT]
XmnI 699 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 60 AGCT AG^CT
801 AGCT
BcnI 245 CCGGG CC^[GC]GG
709 CCCGG
BsmI 178 GAATGC GAATGC(1/-1)
699 GAATGC
Cac8I 148 GCTAGC GC[ACGT]^[ACGT]GC
286 GCAGGC
DdeI 346 CTGAG C^T[ACGT]AG
567 CTCAG
DsaI 293 CCGTGG C^C[GA][CT]GG
644 CCACGG
EaeI 224 TGGCCA [CT]^GGCC[GA]
492 TGGCCG
HapII 245 CCGG C^CGG
710 CCGG
HpaII 245 CCGG C^CGG
710 CCGG
MaeIII 332 GTGAC ^GT[ACGT]AC
624 GTCAC
MboII 883 GAAGA GAAGA(8/7)
401 TCTTC
MseI 476 TTAA T^TAA
872 TTAA
MslI 525 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
645 CACGGGTGTG
MspI 245 CCGG C^CGG
710 CCGG
NciI 245 CCGGG CC^[GC]GG
709 CCCGG
NspI 273 GCATGT [GA]CATG^[CT]
764 ACATGT
PleI 203 GAGTC GAGTC(4/5)
876 GAGTC
SmlI 156 CTTGAG C^T[CT][GA]AG
304 CTTGAG
Tru9I 476 TTAA T^TAA
872 TTAA
Tsp45I 332 GTGAC ^GT[GC]AC
624 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI BanI BbvI BsaJI
BsgI BsmFI BsrI BssKI BstF5I
BstNI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MboI
MnlI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI SfaNI
TseI Tsp509I TspRI