Restriction map for
pF1KB9728
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
Ksp632I MamI MfeI MluI MroI
MunI NdeI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 948 GGTACC G^GTACC
AflIII 824 ACATGT A^C[GA][CT]GT
AlwNI 382 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 484 CCTAAGG CC^T[ACGT]AGG
Asp718I 948 GGTACC G^GTACC
BbvCI 1262 CCTCAGC CCTCAGC(-5/-2)
BfaI 1268 CTAG C^TAG
BglII 374 AGATCT A^GATCT
BlpI 366 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 366 GCTCAGC GC^T[ACGT]AGC
BsaI 127 GAGACC GGTCTC(1/5)
BsmAI 127 GAGAC GTCTC(1/5)
BsmI 982 GAATGC GAATGC(1/-1)
BspLU11I 824 ACATGT A^CATGT
BssSI 999 CACGAG CACGAG(-5/-1)
BstXI 845 CCACCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 374 AGATCT [GA]^GATC[CT]
Bsu36I 484 CCTAAGG CC^T[ACGT]AGG
CelII 366 GCTCAGC GC^T[ACGT]AGC
DpnII 375 GATC ^GATC
DraIII 622 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 484 CCTAAGG CC^T[ACGT]AGG
EcoT14I 965 CCATGG C^C[AT][AT]GG
KpnI 948 GGTACC GGTAC^C
MaeI 1268 CTAG C^TAG
MaeII 815 ACGT A^CGT
MboI 375 GATC ^GATC
MflI 374 AGATCT [GA]^GATC[CT]
MseI 1316 TTAA T^TAA
NcoI 965 CCATGG C^CATGG
NdeII 375 GATC ^GATC
NheI 1267 GCTAGC G^CTAGC
NruI 1155 TCGCGA TCG^CGA
PflMI 621 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 388 CTGCAG CTGCA^G
PvuII 858 CAGCTG CAG^CTG
Sau3AI 375 GATC ^GATC
SbfI 387 CCTGCAGG CCTGCA^GG
SfcI 388 CTGCAG C^T[GA][CT]AG
SgrAI 762 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 387 CCTGCAGG CCTGCA^GG
StuI 1095 AGGCCT AGG^CCT
StyI 965 CCATGG C^C[AT][AT]GG
Tru9I 1316 TTAA T^TAA
Tsp45I 1211 GTGAC ^GT[GC]AC
Tsp509I 190 AATT ^AATT
Van91I 621 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 374 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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BalI 332 TGGCCA TGG^CCA
854 TGGCCA
BsgI 1099 CTGCAC GTGCAG(16/14)
45 GTGCAG
BsiEI 574 CGGCCG CG[GA][CT]^CG
896 CGGCCG
BssHII 62 GCGCGC G^CGCGC
394 GCGCGC
BstF5I 336 CATCC GGATG(2/0)
1258 CATCC
EagI 574 CGGCCG C^GGCCG
896 CGGCCG
EclXI 574 CGGCCG C^GGCCG
896 CGGCCG
Eco52I 574 CGGCCG C^GGCCG
896 CGGCCG
FokI 336 CATCC GGATG(9/13)
1258 CATCC
MaeIII 1184 GTTAC ^GT[ACGT]AC
1211 GTGAC
MluNI 332 TGGCCA TGG^CCA
854 TGGCCA
MscI 332 TGGCCA TGG^CCA
854 TGGCCA
MslI 324 CATCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
846 CACCTACATG
NspI 824 ACATGT [GA]CATG^[CT]
1109 GCATGT
PpuMI 876 GGGACCC [GA]G^G[AT]CC[CT]
1160 AGGACCT
SfaNI 744 GATGC GCATC(5/9)
1191 GCATC
SmaI 837 CCCGGG CCC^GGG
880 CCCGGG
SmlI 607 CTCAAG C^T[CT][GA]AG
1045 CTCAAG
XmaCI 837 CCCGGG C^CCGGG
880 CCCGGG
XmaI 837 CCCGGG C^CCGGG
880 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI KspI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIII NlaIV RsaI SacI
SacII Sau96I ScrFI SfoI TaqI
TseI TspRI TthHB8I