Restriction map for
pF1KE0526
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI MwoI NaeI
NarI NcoI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AciI 396 GCGG CCGC(-3/-1)
AfaI 267 GTAC GT^AC
Asp700I 507 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 440 GTGCTC G[AT]GC[AT]^C
AvaII 33 GGACC G^G[AT]CC
BanII 153 GAGCCC G[GA]GC[CT]^C
BbvCI 418 GCTGAGG CCTCAGC(-5/-2)
BfaI 361 CTAG C^TAG
BsgI 377 CTGCAC GTGCAG(16/14)
BsiHKAI 440 GTGCTC G[AT]GC[AT]^C
BspMI 436 GCAGGT ACCTGC(4/8)
BsrBI 394 GAGCGG CCGCTC(-3/-3)
EarI 14 GAAGAG CTCTTC(1/4)
EcoT14I 426 CCAAGG C^C[AT][AT]GG
Ksp632I 14 GAAGAG CTCTTC(1/4)
MaeI 361 CTAG C^TAG
NdeI 84 CATATG CA^TATG
PflMI 426 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 330 CTGCAG CTGCA^G
RsaI 267 GTAC GT^AC
SapI 14 GAAGAGC GCTCTTC(1/4)
SfcI 330 CTGCAG C^T[GA][CT]AG
StyI 426 CCAAGG C^C[AT][AT]GG
TaqI 451 TCGA T^CGA
Tsp45I 399 GTCAC ^GT[GC]AC
TspRI 75 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 451 TCGA T^CGA
Van91I 426 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 507 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 98 GGATC GGATC(4/5)
106 GGATC
AvaI 224 CCCGGG C^[CT]CG[GA]G
355 CCCGGG
BglII 179 AGATCT A^GATCT
509 AGATCT
BmyI 153 GAGCCC G[AGT]GC[ACT]^C
440 GTGCTC
BpmI 47 CTCCAG CTGGAG(16/14)
207 CTGGAG
BsoBI 224 CCCGGG C^[CT]CG[GA]G
355 CCCGGG
Bsp1286I 153 GAGCCC G[AGT]GC[ACT]^C
440 GTGCTC
Cac8I 90 GCAGGC GC[ACGT]^[ACGT]GC
325 GCCAGC
Cfr13I 33 GGACC G^G[ACGT]CC
423 GGCCC
HapII 225 CCGG C^CGG
356 CCGG
HinfI 53 GATTC G^A[ACGT]TC
462 GAATC
HpaII 225 CCGG C^CGG
356 CCGG
MaeIII 295 GTAAC ^GT[ACGT]AC
399 GTCAC
MseI 236 TTAA T^TAA
248 TTAA
MspA1I 327 CAGCTG C[AC]G^C[GT]G
374 CAGCTG
MspI 225 CCGG C^CGG
356 CCGG
NlaIII 128 CATG CATG^
403 CATG
PvuII 327 CAGCTG CAG^CTG
374 CAGCTG
Sau96I 33 GGACC G^G[ACGT]CC
423 GGCCC
SmaI 224 CCCGGG CCC^GGG
355 CCCGGG
TfiI 53 GATTC G^A[AT]TC
462 GAATC
Tru9I 236 TTAA T^TAA
248 TTAA
XmaCI 224 CCCGGG C^CCGGG
355 CCCGGG
XmaI 224 CCCGGG C^CCGGG
355 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AlwNI BbvI BcnI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI BstYI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII ItaI MboI
MboII MflI MnlI MvaI NciI
NdeII Sau3AI ScrFI SfaNI TseI
XhoII