Restriction map for
pF1KB6742
##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MaeIII
MamI MfeI MflI MluI MroI
MseI MslI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfaNI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 137 CGCG CG^CG
AfaI 31 GTAC GT^AC
AlwI 315 GATCC GGATC(4/5)
AlwNI 271 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 347 GAGCTC G[AT]GC[AT]^C
BalI 56 TGGCCA TGG^CCA
BanII 347 GAGCTC G[GA]GC[CT]^C
BbsI 229 GTCTTC GAAGAC(2/6)
BcnI 18 CCGGG CC^[GC]GG
BfaI 328 CTAG C^TAG
BglI 64 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 6 GCTGAGC GC^T[ACGT]AGC
BmyI 347 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 6 GCTGAGC GC^T[ACGT]AGC
BpuAI 229 GTCTTC GAAGAC(2/6)
BsgI 154 CTGCAC GTGCAG(16/14)
BsiHKAI 347 GAGCTC G[AT]GC[AT]^C
BsiYI 323 CCGAGCTAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 323 CCGAGCTAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 347 GAGCTC G[AGT]GC[ACT]^C
BsrBI 340 GAGCGG CCGCTC(-3/-3)
BstUI 137 CGCG CG^CG
CelII 6 GCTGAGC GC^T[ACGT]AGC
DraII 20 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 27 GACAGTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 20 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 237 CCTTGG C^C[AT][AT]GG
HaeII 49 AGCGCC [GA]GCGC^[CT]
HapII 18 CCGG C^CGG
HinfI 360 GATTC G^A[ACGT]TC
HpaII 18 CCGG C^CGG
HphI 312 GGTGA GGTGA(8/7)
MaeI 328 CTAG C^TAG
MluNI 56 TGGCCA TGG^CCA
MscI 56 TGGCCA TGG^CCA
MspI 18 CCGG C^CGG
MvnI 137 CGCG CG^CG
MwoI 64 GCCACGGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 18 CCGGG CC^[GC]GG
NlaIII 159 CATG CATG^
NspI 158 ACATGC [GA]CATG^[CT]
PpuMI 20 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 118 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 91 CTGCAG CTGCA^G
RsaI 31 GTAC GT^AC
SacI 347 GAGCTC GAGCT^C
ScaI 30 AGTACT AGT^ACT
SfcI 91 CTGCAG C^T[GA][CT]AG
SfiI 63 GGCCACGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 237 CCTTGG C^C[AT][AT]GG
TaqI 386 TCGA T^CGA
TfiI 360 GATTC G^A[AT]TC
TspRI 121 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 386 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
Cac8I 206 GCCTGC GC[ACGT]^[ACGT]GC
305 GCAAGC
DpnII 96 GATC ^GATC
315 GATC
EaeI 56 TGGCCA [CT]^GGCC[GA]
62 CGGCCA
FauI 111 GCGGG CCCGC(4/6)
342 GCGGG
MboI 96 GATC ^GATC
315 GATC
MboII 181 GAAGA GAAGA(8/7)
230 TCTTC
NdeII 96 GATC ^GATC
315 GATC
Sau3AI 96 GATC ^GATC
315 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsmFI BssKI BstF5I BstNI CfoI
Cfr13I DdeI DsaI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I ItaI
MnlI MvaI NlaIV Sau96I ScrFI
TseI