Restriction map for
pF1KB6116
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BbrPI BbvCI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsiWI BsmI Bsp1407I BspDI
BspHI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI MfeI MluI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Tsp45I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 143 GGTACC G^GTACC
AccI 1690 GTCGAC GT^[AC][GT]AC
AccIII 1554 TCCGGA T^CCGGA
AfaI 144 GTAC GT^AC
AflIII 1646 ACATGT A^C[GA][CT]GT
Alw44I 781 GTGCAC G^TGCAC
ApaLI 781 GTGCAC G^TGCAC
Asp718I 143 GGTACC G^GTACC
BalI 266 TGGCCA TGG^CCA
BbeI 287 GGCGCC GGCGC^C
BclI 629 TGATCA T^GATCA
BfaI 808 CTAG C^TAG
BsaBI 684 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1554 TCCGGA T^CCGGA
BsiEI 107 CGGCCG CG[GA][CT]^CG
BsmBI 811 GAGACG CGTCTC(1/5)
BspEI 1554 TCCGGA T^CCGGA
BspLU11I 1646 ACATGT A^CATGT
BssSI 1735 CACGAG CACGAG(-5/-1)
BstAPI 51 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1070 AGATCC [GA]^GATC[CT]
DrdI 604 GACACCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 107 CGGCCG C^GGCCG
EarI 904 CTCTTC CTCTTC(1/4)
EclXI 107 CGGCCG C^GGCCG
Eco52I 107 CGGCCG C^GGCCG
EcoNI 1530 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 260 CCTTGG C^C[AT][AT]GG
FbaI 629 TGATCA T^GATCA
HaeII 287 GGCGCC [GA]GCGC^[CT]
HindIII 1189 AAGCTT A^AGCTT
KasI 287 GGCGCC G^GCGCC
KpnI 143 GGTACC GGTAC^C
Ksp632I 904 CTCTTC CTCTTC(1/4)
KspI 8 CCGCGG CCGC^GG
MaeI 808 CTAG C^TAG
MaeIII 959 GTTAC ^GT[ACGT]AC
MamI 684 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1070 AGATCC [GA]^GATC[CT]
MluNI 266 TGGCCA TGG^CCA
MroI 1554 TCCGGA T^CCGGA
MscI 266 TGGCCA TGG^CCA
NarI 287 GGCGCC GG^CGCC
PvuII 1243 CAGCTG CAG^CTG
RsaI 144 GTAC GT^AC
SacII 8 CCGCGG CCGC^GG
SalI 1690 GTCGAC G^TCGAC
SbfI 1326 CCTGCAGG CCTGCA^GG
SfoI 287 GGCGCC GGC^GCC
Sse8387I 1326 CCTGCAGG CCTGCA^GG
StyI 260 CCTTGG C^C[AT][AT]GG
Tsp509I 1020 AATT ^AATT
XhoII 1070 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 9 CGCG CG^CG
97 CGCG
AlwI 735 GATCC GGATC(4/5)
1071 GATCC
BbsI 457 GAAGAC GAAGAC(2/6)
1519 GAAGAC
BcgI 563 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
899 GCACCCTCTTCG
BpuAI 457 GAAGAC GAAGAC(2/6)
1519 GAAGAC
BsaI 1168 GAGACC GGTCTC(1/5)
1180 GAGACC
BsaWI 405 ACCGGA [AT]^CCGG[AT]
1554 TCCGGA
BspMI 1171 ACCTGC ACCTGC(4/8)
468 GCAGGT
BsrBI 337 GAGCGG CCGCTC(-3/-3)
1726 GAGCGG
BsrDI 479 CATTGC GCAATG(2/0)
1175 GCAATG
BstUI 9 CGCG CG^CG
97 CGCG
DdeI 1166 CTGAG C^T[ACGT]AG
1628 CTGAG
HincII 331 GTCAAC GT[CT]^[GA]AC
1690 GTCGAC
HindII 331 GTCAAC GT[CT]^[GA]AC
1690 GTCGAC
MspA1I 8 CCGCGG C[AC]G^C[GT]G
1243 CAGCTG
MvnI 9 CGCG CG^CG
97 CGCG
PleI 1284 GACTC GAGTC(4/5)
1406 GAGTC
SfaNI 409 GATGC GCATC(5/9)
380 GCATC
SmlI 1583 CTCAAG C^T[CT][GA]AG
1699 CTCAAG
TfiI 238 GATTC G^A[AT]TC
910 GATTC
XcmI 363 CCACCTGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1383 CCACGGGGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcyI AluI AlwNI ApaI
AspHI AspI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PpuMI PstI
SacI Sau3AI Sau96I ScrFI SfcI
SmaI StuI TaqI TseI TspRI
Tth111I TthHB8I XmaCI XmaI