Restriction map for
pF1KB6525
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I MaeI MaeII
MamI MboII MfeI MluI MluNI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 684 TCCGGA T^CCGGA
AcsI 423 GAATTT [GA]^AATT[CT]
AocI 855 CCTCAGG CC^T[ACGT]AGG
ApoI 423 GAATTT [GA]^AATT[CT]
BamHI 763 GGATCC G^GATCC
BbvCI 16 CCTCAGC CCTCAGC(-5/-2)
BglII 267 AGATCT A^GATCT
BsaI 259 GAGACC GGTCTC(1/5)
BsaWI 684 TCCGGA [AT]^CCGG[AT]
BseAI 684 TCCGGA T^CCGGA
BsgI 829 GTGCAG GTGCAG(16/14)
BsmBI 1010 GAGACG CGTCTC(1/5)
BspEI 684 TCCGGA T^CCGGA
BspHI 419 TCATGA T^CATGA
BspMI 658 ACCTGC ACCTGC(4/8)
BsrFI 730 ACCGGC [GA]^CCGG[CT]
BssSI 895 CTCGTG CACGAG(-5/-1)
BstXI 985 CCAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 855 CCTCAGG CC^T[ACGT]AGG
Cfr10I 730 ACCGGC [GA]^CCGG[CT]
DrdI 1015 GACACCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 855 CCTCAGG CC^T[ACGT]AGG
EcoNI 432 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 991 CCTTGG C^C[AT][AT]GG
MroI 684 TCCGGA T^CCGGA
MslI 375 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 129 GACTC GAGTC(4/5)
RcaI 419 TCATGA T^CATGA
SexAI 233 ACCAGGT A^CC[AT]GGT
SfaNI 1054 GATGC GCATC(5/9)
SmaI 123 CCCGGG CCC^GGG
SmlI 433 CTCAAG C^T[CT][GA]AG
StyI 991 CCTTGG C^C[AT][AT]GG
TaqI 428 TCGA T^CGA
Tsp509I 424 AATT ^AATT
TspRI 756 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 428 TCGA T^CGA
XmaCI 123 CCCGGG C^CCGGG
XmaI 123 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 93 GTAC GT^AC
171 GTAC
ApaI 159 GGGCCC GGGCC^C
363 GGGCCC
AvaI 123 CCCGGG C^[CT]CG[GA]G
463 CCCGAG
BglI 188 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
344 GCCTGCTGGGC
BsmAI 259 GAGAC GTCTC(1/5)
1010 GAGAC
BsoBI 123 CCCGGG C^[CT]CG[GA]G
463 CCCGAG
BsrI 360 ACTGG ACTGG(1/-1)
458 ACTGG
BstAPI 339 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
549 GCACCGCCTGC
HaeII 27 GGCGCT [GA]GCGC^[CT]
664 AGCGCC
HindIII 299 AAGCTT A^AGCTT
308 AAGCTT
HphI 739 GGTGA GGTGA(8/7)
524 TCACC
NlaIII 420 CATG CATG^
612 CATG
PstI 337 CTGCAG CTGCA^G
660 CTGCAG
RsaI 93 GTAC GT^AC
171 GTAC
SacI 520 GAGCTC GAGCT^C
950 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
KspI MaeIII MboI MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PpuMI SacII
Sau3AI Sau96I ScrFI SfcI TfiI
TseI Tsp45I XhoII