################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 369 GGCGCC G[GA]^CG[CT]C AflIII 104 ACGTGT A^C[GA][CT]GT AlwNI 81 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 183 GGGCCC GGGCC^C BanI 369 GGCGCC G^G[CT][GA]CC BbeI 369 GGCGCC GGCGC^C BcgI 376 GCACCGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 52 CTAG C^TAG BsaHI 369 GGCGCC G[GA]^CG[CT]C BsaI 532 GAGACC GGTCTC(1/5) BsiEI 395 CGATCG CG[GA][CT]^CG BsmAI 532 GAGAC GTCTC(1/5) BsrBI 650 CCGCTC CCGCTC(-3/-3) BsrI 251 ACTGG ACTGG(1/-1) BssHII 490 GCGCGC G^CGCGC BssSI 292 CACGAG CACGAG(-5/-1) BstF5I 708 GGATG GGATG(2/0) DraIII 100 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 355 CCACGG C^C[GA][CT]GG EcoNI 51 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 708 GGATG GGATG(9/13) HaeII 369 GGCGCC [GA]GCGC^[CT] HgaI 680 GCGTC GACGC(5/10) Hin1I 369 GGCGCC G[GA]^CG[CT]C HinfI 390 GACTC G^A[ACGT]TC KasI 369 GGCGCC G^GCGCC MaeI 52 CTAG C^TAG MaeII 104 ACGT A^CGT MaeIII 469 GTGAC ^GT[ACGT]AC MslI 416 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 369 GGCGCC GG^CGCC PflMI 206 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 390 GACTC GAGTC(4/5) PstI 282 CTGCAG CTGCA^G PvuI 395 CGATCG CGAT^CG SapI 69 GAAGAGC GCTCTTC(1/4) SfcI 282 CTGCAG C^T[GA][CT]AG SfoI 369 GGCGCC GGC^GCC SmlI 256 CTCAAG C^T[CT][GA]AG Tsp45I 469 GTGAC ^GT[GC]AC TspRI 153 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 206 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 14 AGCT AG^CT 95 AGCT AvaI 384 CTCGGG C^[CT]CG[GA]G 462 CTCGGG BanII 183 GGGCCC G[GA]GC[CT]^C 578 GAGCCC BmyI 183 GGGCCC G[AGT]GC[ACT]^C 578 GAGCCC BsoBI 384 CTCGGG C^[CT]CG[GA]G 462 CTCGGG Bsp1286I 183 GGGCCC G[AGT]GC[ACT]^C 578 GAGCCC BspMI 85 ACCTGC ACCTGC(4/8) 231 GCAGGT BsrFI 287 GCCGGC [GA]^CCGG[CT] 540 GCCGGC Cfr10I 287 GCCGGC [GA]^CCGG[CT] 540 GCCGGC DpnII 396 GATC ^GATC 591 GATC EarI 40 CTCTTC CTCTTC(1/4) 69 GAAGAG HaeIII 184 GGCC GG^CC 477 GGCC Ksp632I 40 CTCTTC CTCTTC(1/4) 69 GAAGAG MboI 396 GATC ^GATC 591 GATC MboII 69 GAAGA GAAGA(8/7) 41 TCTTC NaeI 287 GCCGGC GCC^GGC 540 GCCGGC NdeII 396 GATC ^GATC 591 GATC NgoMIV 287 GCCGGC G^CCGGC 540 GCCGGC Sau3AI 396 GATC ^GATC 591 GATC SfaNI 709 GATGC GCATC(5/9) 278 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AvaII BbvI BcnI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HapII HhaI HinP1I HpaII HphI ItaI MnlI MspI MvaI MvnI MwoI NciI NlaIV PpuMI Sau96I ScrFI TseI