Restriction map for
pF1KB6559
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BalI BbeI BbvCI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NarI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 892 AAATTC [GA]^AATT[CT]
AflIII 802 ACGTGT A^C[GA][CT]GT
AhdI 452 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 474 CCTCAGG CC^T[ACGT]AGG
ApaI 767 GGGCCC GGGCC^C
ApoI 892 AAATTC [GA]^AATT[CT]
AspEI 452 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1016 GGATCC G^GATCC
BanI 51 GGCACC G^G[CT][GA]CC
BbsI 147 GAAGAC GAAGAC(2/6)
BcgI 685 CGACCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 147 GAAGAC GAAGAC(2/6)
BsmI 964 GAATGC GAATGC(1/-1)
BsrDI 387 CATTGC GCAATG(2/0)
BsrFI 219 GCCGGC [GA]^CCGG[CT]
BstAPI 1079 GCAAGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1016 GGATCC [GA]^GATC[CT]
Bsu36I 474 CCTCAGG CC^T[ACGT]AGG
Cfr10I 219 GCCGGC [GA]^CCGG[CT]
DpnII 1017 GATC ^GATC
Eam1105I 452 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 474 CCTCAGG CC^T[ACGT]AGG
EcoNI 771 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 966 ATGCAT ATGCA^T
MaeIII 808 GTGAC ^GT[ACGT]AC
MboI 1017 GATC ^GATC
MflI 1016 GGATCC [GA]^GATC[CT]
MslI 801 CACGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 219 GCCGGC GCC^GGC
NcoI 482 CCATGG C^CATGG
NdeII 1017 GATC ^GATC
NgoMIV 219 GCCGGC G^CCGGC
NsiI 966 ATGCAT ATGCA^T
PflMI 179 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 450 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 1017 GATC ^GATC
StuI 779 AGGCCT AGG^CCT
Tsp45I 808 GTGAC ^GT[GC]AC
Van91I 179 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1016 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 59 CGCG CG^CG
64 CGCG
AfaI 609 GTAC GT^AC
867 GTAC
AlwI 1017 GATCC GGATC(4/5)
1016 GGATC
BanII 767 GGGCCC G[GA]GC[CT]^C
1033 GAGCCC
BbrPI 627 CACGTG CAC^GTG
801 CACGTG
BmyI 767 GGGCCC G[AGT]GC[ACT]^C
1033 GAGCCC
BpmI 332 CTCCAG CTGGAG(16/14)
718 CTGGAG
BsaAI 627 CACGTG [CT]AC^GT[GA]
801 CACGTG
BsgI 224 CTGCAC GTGCAG(16/14)
406 CTGCAC
Bsp1286I 767 GGGCCC G[AGT]GC[ACT]^C
1033 GAGCCC
BspMI 637 ACCTGC ACCTGC(4/8)
811 ACCTGC
BstUI 59 CGCG CG^CG
64 CGCG
BstXI 648 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCAGAAGGATGG
CfoI 60 GCGC GCG^C
815 GCGC
EaeI 71 TGGCCG [CT]^GGCC[GA]
117 TGGCCG
EcoT14I 482 CCATGG C^C[AT][AT]GG
1044 CCAAGG
HhaI 60 GCGC GCG^C
815 GCGC
HinP1I 60 GCGC G^CGC
815 GCGC
HphI 280 GGTGA GGTGA(8/7)
44 TCACC
MvnI 59 CGCG CG^CG
64 CGCG
PmaCI 627 CACGTG CAC^GTG
801 CACGTG
PmlI 627 CACGTG CAC^GTG
801 CACGTG
PvuII 403 CAGCTG CAG^CTG
649 CAGCTG
RsaI 609 GTAC GT^AC
867 GTAC
SapI 490 GAAGAGC GCTCTTC(1/4)
237 GCTCTTC
StyI 482 CCATGG C^C[AT][AT]GG
1044 CCAAGG
TaqI 962 TCGA T^CGA
1031 TCGA
Tsp509I 893 AATT ^AATT
991 AATT
TthHB8I 962 TCGA T^CGA
1031 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwNI AvaI AvaII
BbvI BcnI BsaJI BseRI BsiYI
BslI BsmFI BsoBI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII DsaI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI Ksp632I MaeII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PstI Sau96I
ScrFI SfaNI SfcI TseI TspRI