Restriction map for
pF1KE0129
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI StuI SwaI TfiI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 604 GTCTAC GT^[AC][GT]AC
AccII 38 CGCG CG^CG
Alw44I 76 GTGCAC G^TGCAC
AlwI 738 GATCC GGATC(4/5)
AlwNI 256 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 76 GTGCAC G^TGCAC
BbeI 60 GGCGCC GGCGC^C
BbsI 222 GTCTTC GAAGAC(2/6)
BfaI 121 CTAG C^TAG
BglI 645 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 454 CTCCAG CTGGAG(16/14)
BpuAI 222 GTCTTC GAAGAC(2/6)
BsgI 364 GTGCAG GTGCAG(16/14)
BsrDI 141 CATTGC GCAATG(2/0)
BsrI 461 ACTGG ACTGG(1/-1)
BssSI 269 CTCGTG CACGAG(-5/-1)
BstAPI 255 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 38 CGCG CG^CG
BstYI 737 AGATCC [GA]^GATC[CT]
EaeI 236 TGGCCG [CT]^GGCC[GA]
EarI 250 GAAGAG CTCTTC(1/4)
HgaI 193 GACGC GACGC(5/10)
KasI 60 GGCGCC G^GCGCC
Ksp632I 250 GAAGAG CTCTTC(1/4)
MaeI 121 CTAG C^TAG
MflI 737 AGATCC [GA]^GATC[CT]
MvnI 38 CGCG CG^CG
NarI 60 GGCGCC GG^CGCC
PflMI 438 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 477 CAGCTG CAG^CTG
SapI 250 GAAGAGC GCTCTTC(1/4)
SbfI 597 CCTGCAGG CCTGCA^GG
SexAI 443 ACCTGGT A^CC[AT]GGT
SfoI 60 GGCGCC GGC^GCC
Sse8387I 597 CCTGCAGG CCTGCA^GG
SspI 694 AATATT AAT^ATT
TaqI 632 TCGA T^CGA
TthHB8I 632 TCGA T^CGA
Van91I 438 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 737 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 60 GGCGCC G[GA]^CG[CT]C
193 GACGCC
AfaI 297 GTAC GT^AC
429 GTAC
ApaI 42 GGGCCC GGGCC^C
512 GGGCCC
AspHI 76 GTGCAC G[AT]GC[AT]^C
583 GTGCTC
AvaII 131 GGTCC G^G[AT]CC
488 GGACC
BanI 60 GGCGCC G^G[CT][GA]CC
618 GGTGCC
BsaHI 60 GGCGCC G[GA]^CG[CT]C
193 GACGCC
BsaI 181 GAGACC GGTCTC(1/5)
405 GGTCTC
BsiHKAI 76 GTGCAC G[AT]GC[AT]^C
583 GTGCTC
BsmAI 181 GAGAC GTCTC(1/5)
406 GTCTC
BsmFI 353 GGGAC GGGAC(10/14)
524 GGGAC
BsrFI 57 GCCGGC [GA]^CCGG[CT]
238 GCCGGC
BstF5I 418 CATCC GGATG(2/0)
125 GGATG
Cfr10I 57 GCCGGC [GA]^CCGG[CT]
238 GCCGGC
DdeI 385 CTGAG C^T[ACGT]AG
638 CTGAG
DpnII 706 GATC ^GATC
738 GATC
EcoT14I 534 CCATGG C^C[AT][AT]GG
725 CCATGG
FokI 418 CATCC GGATG(9/13)
125 GGATG
HaeII 60 GGCGCC [GA]GCGC^[CT]
540 GGCGCT
Hin1I 60 GGCGCC G[GA]^CG[CT]C
193 GACGCC
HinfI 267 GACTC G^A[ACGT]TC
331 GACTC
HphI 313 GGTGA GGTGA(8/7)
503 TCACC
MaeIII 272 GTGAC ^GT[ACGT]AC
502 GTCAC
MboI 706 GATC ^GATC
738 GATC
MboII 250 GAAGA GAAGA(8/7)
223 TCTTC
MseI 302 TTAA T^TAA
662 TTAA
MslI 279 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
570 CATCGCCGTG
MspA1I 477 CAGCTG C[AC]G^C[GT]G
484 CAGCGG
NaeI 57 GCCGGC GCC^GGC
238 GCCGGC
NcoI 534 CCATGG C^CATGG
725 CCATGG
NdeII 706 GATC ^GATC
738 GATC
NgoMIV 57 GCCGGC G^CCGGC
238 GCCGGC
PleI 267 GACTC GAGTC(4/5)
331 GACTC
PstI 32 CTGCAG CTGCA^G
598 CTGCAG
RsaI 297 GTAC GT^AC
429 GTAC
Sau3AI 706 GATC ^GATC
738 GATC
SfcI 32 CTGCAG C^T[GA][CT]AG
598 CTGCAG
StyI 534 CCATGG C^C[AT][AT]GG
725 CCATGG
Tru9I 302 TTAA T^TAA
662 TTAA
Tsp45I 272 GTGAC ^GT[GC]AC
502 GTCAC
Tsp509I 228 AATT ^AATT
326 AATT
TspRI 147 CACTG CA[GC]TG[ACGT][ACGT]^
367 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BspMI BssKI BstNI Cac8I CfoI
Cfr13I DraII DsaI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI