##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          148  GAATTT               [GA]^AATT[CT]
AlwNI         413  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          148  GAATTT               [GA]^AATT[CT]
AspHI         225  GAGCTC               G[AT]GC[AT]^C
AvaII          31  GGTCC                G^G[AT]CC
BanII         225  GAGCTC               G[GA]GC[CT]^C
BbsI           42  GTCTTC               GAAGAC(2/6)
BbvI          685  GCTGC                GCAGC(8/12)
BmyI          225  GAGCTC               G[AGT]GC[ACT]^C
BpuAI          42  GTCTTC               GAAGAC(2/6)
BseRI         574  GAGGAG               GAGGAG(10/8)
BsiHKAI       225  GAGCTC               G[AT]GC[AT]^C
BsmFI         698  GTCCC                GGGAC(10/14)
Bsp1286I      225  GAGCTC               G[AGT]GC[ACT]^C
BsrDI          13  GCAATG               GCAATG(2/0)
BssKI          89  CCTGG                ^CC[ACGT]GG
BstF5I        230  CATCC                GGATG(2/0)
BstNI          89  CCTGG                CC^[AT]GG
BstXI         134  CCAAGGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         582  GCGAGC               GC[ACGT]^[ACGT]GC
DpnII          23  GATC                 ^GATC
DraI          441  TTTAAA               TTT^AAA
EcoRII         89  CCTGG                ^CC[AT]GG
Fnu4HI        685  GCTGC                GC^[ACGT]GC
FokI          230  CATCC                GGATG(9/13)
HphI          706  TCACC                GGTGA(8/7)
ItaI          685  GCTGC                GC^[ACGT]GC
MboI           23  GATC                 ^GATC
MvaI           89  CCTGG                CC^[AT]GG
NdeII          23  GATC                 ^GATC
NlaIII        408  CATG                 CATG^
SacI          225  GAGCTC               GAGCT^C
Sau3AI         23  GATC                 ^GATC
ScrFI          89  CCTGG                CC^[ACGT]GG
StuI           82  AGGCCT               AGG^CCT
TaqI           75  TCGA                 T^CGA
TseI          685  GCTGC                G^C[AT]GC
TspRI         603  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        75  TCGA                 T^CGA
XcmI           79  CCAAGGCCTACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BfaI          112  CTAG                 C^TAG
              689  CTAG                
Cfr13I         31  GGTCC                G^G[ACGT]CC
               49  GGGCC               
EcoT14I        79  CCAAGG               C^C[AT][AT]GG
              134  CCAAGG              
HaeIII         50  GGCC                 GG^CC
               83  GGCC                
MaeI          112  CTAG                 C^TAG
              689  CTAG                
NlaIV          30  GGGTCC               GG[ACGT]^[ACGT]CC
              130  GGCTCC              
Sau96I         31  GGTCC                G^G[ACGT]CC
               49  GGGCC               
StyI           79  CCAAGG               C^C[AT][AT]GG
              134  CCAAGG              
Tsp509I       149  AATT                 ^AATT
              206  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BsaJI       BsrI        DdeI        HinfI       
MboII       MnlI        MseI        PleI        TfiI        
Tru9I