Restriction map for
pF1KE0141
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tsp509I
TspRI XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 189 GTAC GT^AC
AgeI 591 ACCGGT A^CCGGT
AlwI 417 GATCC GGATC(4/5)
Aor51HI 239 AGCGCT AGC^GCT
Asp700I 366 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 101 TGCGCA TGC^GCA
BpmI 214 CTGGAG CTGGAG(16/14)
BsaAI 277 TACGTG [CT]AC^GT[GA]
BsaWI 591 ACCGGT [AT]^CCGG[AT]
BsmAI 481 GAGAC GTCTC(1/5)
BspMI 200 ACCTGC ACCTGC(4/8)
BsrFI 591 ACCGGT [GA]^CCGG[CT]
BsrI 568 ACTGG ACTGG(1/-1)
BssSI 208 CACGAG CACGAG(-5/-1)
BstAPI 236 GCAAGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 416 AGATCC [GA]^GATC[CT]
Cfr10I 591 ACCGGT [GA]^CCGG[CT]
DraI 649 TTTAAA TTT^AAA
EaeI 266 CGGCCA [CT]^GGCC[GA]
EarI 366 GAAGAG CTCTTC(1/4)
Eco47III 239 AGCGCT AGC^GCT
FspI 101 TGCGCA TGC^GCA
HaeII 239 AGCGCT [GA]GCGC^[CT]
HincII 454 GTCAAC GT[CT]^[GA]AC
HindII 454 GTCAAC GT[CT]^[GA]AC
Ksp632I 366 GAAGAG CTCTTC(1/4)
MflI 416 AGATCC [GA]^GATC[CT]
MslI 174 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 269 CCATGG C^CATGG
PflFI 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 207 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 591 ACCGGT A^CCGGT
PleI 357 GACTC GAGTC(4/5)
RsaI 189 GTAC GT^AC
SapI 366 GAAGAGC GCTCTTC(1/4)
SmaI 314 CCCGGG CCC^GGG
SmlI 448 CTCAAG C^T[CT][GA]AG
TaqI 614 TCGA T^CGA
TfiI 5 GAATC G^A[AT]TC
Tth111I 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 614 TCGA T^CGA
Van91I 207 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 269 CCATGGACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 416 AGATCC [GA]^GATC[CT]
XmaCI 314 CCCGGG C^CCGGG
XmaI 314 CCCGGG C^CCGGG
XmnI 366 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 378 GGCGTC G[GA]^CG[CT]C
573 GACGCC
AvaII 84 GGACC G^G[AT]CC
589 GGACC
BanII 121 GAGCCC G[GA]GC[CT]^C
165 GGGCTC
BsaHI 378 GGCGTC G[GA]^CG[CT]C
573 GACGCC
BsgI 202 CTGCAC GTGCAG(16/14)
322 GTGCAG
Cfr13I 84 GGACC G^G[ACGT]CC
589 GGACC
DdeI 466 CTCAG C^T[ACGT]AG
530 CTAAG
DsaI 146 CCACGG C^C[GA][CT]GG
269 CCATGG
FauI 32 CCCGC CCCGC(4/6)
184 CCCGC
HgaI 573 GACGC GACGC(5/10)
379 GCGTC
Hin1I 378 GGCGTC G[GA]^CG[CT]C
573 GACGCC
HinfI 5 GAATC G^A[ACGT]TC
357 GACTC
HphI 228 GGTGA GGTGA(8/7)
478 GGTGA
MseI 596 TTAA T^TAA
650 TTAA
Sau96I 84 GGACC G^G[ACGT]CC
589 GGACC
SfaNI 99 GATGC GCATC(5/9)
16 GCATC
Tru9I 596 TTAA T^TAA
650 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII Sau3AI ScrFI StyI TseI