Restriction map for
pF1KB6391
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NlaIII NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwI 249 GGATC GGATC(4/5)
AvaII 286 GGACC G^G[AT]CC
BbsI 701 GTCTTC GAAGAC(2/6)
BbvI 199 GCAGC GCAGC(8/12)
BciVI 229 GGATAC GTATCC(6/5)
BglI 304 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 701 GTCTTC GAAGAC(2/6)
BsaJI 20 CCGTGG C^C[ACGT][ACGT]GG
BsaWI 278 TCCGGT [AT]^CCGG[AT]
BseRI 637 GAGGAG GAGGAG(10/8)
BsgI 453 CTGCAC GTGCAG(16/14)
BsiYI 220 CCTGATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 220 CCTGATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 486 TGTACA T^GTACA
BsrDI 324 CATTGC GCAATG(2/0)
BsrGI 486 TGTACA T^GTACA
BstAPI 190 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 249 GGATCT [GA]^GATC[CT]
DsaI 20 CCGTGG C^C[GA][CT]GG
EcoNI 220 CCTGATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 91 GAATTC G^AATTC
EcoT22I 372 ATGCAT ATGCA^T
HaeIII 303 GGCC GG^CC
HapII 279 CCGG C^CGG
HindIII 388 AAGCTT A^AGCTT
HinfI 275 GATTC G^A[ACGT]TC
HpaII 279 CCGG C^CGG
HphI 88 GGTGA GGTGA(8/7)
MaeII 233 ACGT A^CGT
MflI 249 GGATCT [GA]^GATC[CT]
MslI 257 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 279 CCGG C^CGG
NsiI 372 ATGCAT ATGCA^T
SexAI 711 ACCAGGT A^CC[AT]GGT
SfcI 167 CTACAG C^T[GA][CT]AG
SspBI 486 TGTACA T^GTACA
SspI 562 AATATT AAT^ATT
TfiI 275 GATTC G^A[AT]TC
TseI 199 GCAGC G^C[AT]GC
XhoII 249 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 128 GTAC GT^AC
487 GTAC
AluI 389 AGCT AG^CT
735 AGCT
BfaI 314 CTAG C^TAG
469 CTAG
Cfr13I 286 GGACC G^G[ACGT]CC
303 GGCCC
DpnII 250 GATC ^GATC
412 GATC
DraI 477 TTTAAA TTT^AAA
540 TTTAAA
EarI 77 GAAGAG CTCTTC(1/4)
298 GAAGAG
HincII 109 GTCAAC GT[CT]^[GA]AC
622 GTTGAC
HindII 109 GTCAAC GT[CT]^[GA]AC
622 GTTGAC
Ksp632I 77 GAAGAG CTCTTC(1/4)
298 GAAGAG
MaeI 314 CTAG C^TAG
469 CTAG
MaeIII 502 GTTAC ^GT[ACGT]AC
766 GTTAC
MboI 250 GATC ^GATC
412 GATC
NdeII 250 GATC ^GATC
412 GATC
NheI 313 GCTAGC G^CTAGC
468 GCTAGC
NlaIV 24 GGAACC GG[ACGT]^[ACGT]CC
658 GGAACC
RsaI 128 GTAC GT^AC
487 GTAC
Sau3AI 250 GATC ^GATC
412 GATC
Sau96I 286 GGACC G^G[ACGT]CC
303 GGCCC
SfaNI 214 GATGC GCATC(5/9)
448 GATGC
TaqI 271 TCGA T^CGA
425 TCGA
TthHB8I 271 TCGA T^CGA
425 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AcsI ApoI BpmI BsrI
BssKI BstF5I BstNI Cac8I EcoRII
Fnu4HI FokI ItaI MboII MnlI
MseI MvaI MwoI ScrFI Tru9I
Tsp509I