Restriction map for
pF1KB7163
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BseRI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tru9I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 870 GTCTAC GT^[AC][GT]AC
AccII 633 CGCG CG^CG
AciI 556 GCGG CCGC(-3/-1)
AgeI 613 ACCGGT A^CCGGT
AspI 1050 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 488 TGGCCA TGG^CCA
BanI 1104 GGTGCC G^G[CT][GA]CC
BbsI 681 GAAGAC GAAGAC(2/6)
BcnI 441 CCCGG CC^[GC]GG
BpuAI 681 GAAGAC GAAGAC(2/6)
BsaI 85 GGTCTC GGTCTC(1/5)
BsaWI 613 ACCGGT [AT]^CCGG[AT]
BsiEI 989 CGGCCG CG[GA][CT]^CG
Bsp1407I 836 TGTACA T^GTACA
BsrBI 554 GAGCGG CCGCTC(-3/-3)
BsrFI 613 ACCGGT [GA]^CCGG[CT]
BsrGI 836 TGTACA T^GTACA
BssSI 41 CTCGTG CACGAG(-5/-1)
BstUI 633 CGCG CG^CG
CfoI 632 GCGC GCG^C
Cfr10I 613 ACCGGT [GA]^CCGG[CT]
DraII 134 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 989 CGGCCG C^GGCCG
EclXI 989 CGGCCG C^GGCCG
Eco52I 989 CGGCCG C^GGCCG
EcoO109I 134 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 778 GAATTC G^AATTC
HhaI 632 GCGC GCG^C
HinP1I 632 GCGC G^CGC
MluNI 488 TGGCCA TGG^CCA
MscI 488 TGGCCA TGG^CCA
MslI 612 CACCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 633 CGCG CG^CG
NciI 441 CCCGG CC^[GC]GG
PflFI 1050 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 613 ACCGGT A^CCGGT
PpuMI 134 AGGTCCT [GA]G^G[AT]CC[CT]
SfaNI 607 GATGC GCATC(5/9)
SgrAI 612 CACCGGTG C[GA]^CCGG[CT]G
SmlI 10 CTCAAG C^T[CT][GA]AG
SspBI 836 TGTACA T^GTACA
TaqI 791 TCGA T^CGA
Tth111I 1050 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 791 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 313 AAATTT [GA]^AATT[CT]
778 GAATTC
AlwI 15 GATCC GGATC(4/5)
880 GATCC
ApoI 313 AAATTT [GA]^AATT[CT]
778 GAATTC
AspHI 28 GAGCTC G[AT]GC[AT]^C
142 GAGCTC
AvaII 135 GGTCC G^G[AT]CC
507 GGTCC
BfaI 139 CTAG C^TAG
362 CTAG
BsgI 521 GTGCAG GTGCAG(16/14)
929 GTGCAG
BsiHKAI 28 GAGCTC G[AT]GC[AT]^C
142 GAGCTC
BstYI 14 AGATCC [GA]^GATC[CT]
879 AGATCC
EarI 70 CTCTTC CTCTTC(1/4)
88 CTCTTC
HapII 442 CCGG C^CGG
614 CCGG
HpaII 442 CCGG C^CGG
614 CCGG
Ksp632I 70 CTCTTC CTCTTC(1/4)
88 CTCTTC
MaeI 139 CTAG C^TAG
362 CTAG
MaeII 248 ACGT A^CGT
734 ACGT
MflI 14 AGATCC [GA]^GATC[CT]
879 AGATCC
MspI 442 CCGG C^CGG
614 CCGG
NlaIV 381 GGTTCC GG[ACGT]^[ACGT]CC
1104 GGTGCC
PflMI 939 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAATGGTTGG
SacI 28 GAGCTC GAGCT^C
142 GAGCTC
SexAI 802 ACCAGGT A^CC[AT]GGT
1148 ACCTGGT
StuI 666 AGGCCT AGG^CCT
740 AGGCCT
Tsp509I 314 AATT ^AATT
779 AATT
Van91I 939 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAATGGTTGG
XhoII 14 AGATCC [GA]^GATC[CT]
879 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI BanII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII PleI RsaI Sau3AI
Sau96I ScrFI TfiI TseI Tsp45I
TspRI