Restriction map for
pF1KB6305
##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MluNI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 36 TCCGGA T^CCGGA
AcsI 81 GAATTC [GA]^AATT[CT]
AgeI 113 ACCGGT A^CCGGT
ApaI 621 GGGCCC GGGCC^C
ApoI 81 GAATTC [GA]^AATT[CT]
Asp700I 204 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BglI 409 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 265 TACGTG [CT]AC^GT[GA]
BseAI 36 TCCGGA T^CCGGA
BseRI 534 CTCCTC GAGGAG(10/8)
BsgI 274 GTGCAG GTGCAG(16/14)
BspEI 36 TCCGGA T^CCGGA
BspMI 500 ACCTGC ACCTGC(4/8)
BsrDI 631 CATTGC GCAATG(2/0)
BstXI 243 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 345 GCGC GCG^C
DpnII 282 GATC ^GATC
DraIII 187 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 486 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 81 GAATTC G^AATTC
FauI 50 CCCGC CCCGC(4/6)
HhaI 345 GCGC GCG^C
HinP1I 345 GCGC G^CGC
HinfI 613 GACTC G^A[ACGT]TC
MboI 282 GATC ^GATC
MroI 36 TCCGGA T^CCGGA
NaeI 322 GCCGGC GCC^GGC
NdeII 282 GATC ^GATC
NgoMIV 322 GCCGGC G^CCGGC
PflMI 630 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 113 ACCGGT A^CCGGT
PleI 613 GACTC GAGTC(4/5)
PstI 487 CTGCAG CTGCA^G
Sau3AI 282 GATC ^GATC
Tsp509I 82 AATT ^AATT
Van91I 630 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 204 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 52 CGCG CG^CG
214 CGCG
AciI 51 CCGC CCGC(-3/-1)
213 CCGC
AfaI 111 GTAC GT^AC
264 GTAC
AlwNI 426 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
481 CAGACCCTG
BanII 546 GGGCTC G[GA]GC[CT]^C
621 GGGCCC
BbsI 204 GAAGAC GAAGAC(2/6)
718 GTCTTC
BmyI 546 GGGCTC G[AGT]GC[ACT]^C
621 GGGCCC
BpuAI 204 GAAGAC GAAGAC(2/6)
718 GTCTTC
BsaI 227 GAGACC GGTCTC(1/5)
729 GGTCTC
BsaWI 36 TCCGGA [AT]^CCGG[AT]
113 ACCGGT
Bsp1286I 546 GGGCTC G[AGT]GC[ACT]^C
621 GGGCCC
BsrFI 113 ACCGGT [GA]^CCGG[CT]
322 GCCGGC
BstUI 52 CGCG CG^CG
214 CGCG
Cfr10I 113 ACCGGT [GA]^CCGG[CT]
322 GCCGGC
DsaI 566 CCATGG C^C[GA][CT]GG
660 CCATGG
EcoT14I 566 CCATGG C^C[AT][AT]GG
660 CCATGG
MaeII 266 ACGT A^CGT
314 ACGT
MslI 136 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
564 CACCATGGTG
MvnI 52 CGCG CG^CG
214 CGCG
MwoI 409 GCCATCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
596 GCTCTGGATGC
NcoI 566 CCATGG C^CATGG
660 CCATGG
NlaIII 567 CATG CATG^
661 CATG
PpuMI 29 AGGACCT [GA]G^G[AT]CC[CT]
148 GGGACCC
RsaI 111 GTAC GT^AC
264 GTAC
SfaNI 602 GATGC GCATC(5/9)
291 GCATC
SfcI 487 CTGCAG C^T[GA][CT]AG
713 CTACAG
StyI 566 CCATGG C^C[AT][AT]GG
660 CCATGG
Tsp45I 611 GTGAC ^GT[GC]AC
667 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AvaII BbvI BcnI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI NciI NlaIV
PvuII Sau96I ScrFI TseI TspRI
XcmI