Restriction map for
pF1KB6502
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BstAPI
BstBI CelII ClaI CpoI DraIII
DrdI DsaI Eco47III EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Van91I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 1114 GTATAC GT^[AC][GT]AC
AcsI 688 GAATTC [GA]^AATT[CT]
AcyI 191 GGCGCC G[GA]^CG[CT]C
AflIII 677 ACGTGT A^C[GA][CT]GT
AhdI 535 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 817 CCTGAGG CC^T[ACGT]AGG
ApoI 688 GAATTC [GA]^AATT[CT]
AspEI 535 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 286 GTGCTC G[AT]GC[AT]^C
AspI 961 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 167 TGGCCA TGG^CCA
BanII 496 GGGCTC G[GA]GC[CT]^C
BbeI 191 GGCGCC GGCGC^C
BcgI 571 GCAACAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 451 CTAG C^TAG
BglII 626 AGATCT A^GATCT
BsaHI 191 GGCGCC G[GA]^CG[CT]C
BsiEI 118 CGGCCG CG[GA][CT]^CG
BsiHKAI 286 GTGCTC G[AT]GC[AT]^C
BspMI 209 ACCTGC ACCTGC(4/8)
BssSI 459 CTCGTG CACGAG(-5/-1)
Bst1107I 1114 GTATAC GTA^TAC
BstEII 205 GGTAACC G^GT[ACGT]ACC
BstPI 205 GGTAACC G^GT[ACGT]ACC
BstYI 626 AGATCT [GA]^GATC[CT]
BstZ17I 1114 GTATAC GTA^TAC
Bsu36I 817 CCTGAGG CC^T[ACGT]AGG
DraI 757 TTTAAA TTT^AAA
DraII 503 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 118 CGGCCG C^GGCCG
Eam1105I 535 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 118 CGGCCG C^GGCCG
Eco52I 118 CGGCCG C^GGCCG
Eco81I 817 CCTGAGG CC^T[ACGT]AGG
EcoNI 195 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 503 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 205 GGTAACC G^GT[ACGT]ACC
EcoRI 688 GAATTC G^AATTC
Hin1I 191 GGCGCC G[GA]^CG[CT]C
KasI 191 GGCGCC G^GCGCC
MaeI 451 CTAG C^TAG
MflI 626 AGATCT [GA]^GATC[CT]
MluNI 167 TGGCCA TGG^CCA
MscI 167 TGGCCA TGG^CCA
MseI 758 TTAA T^TAA
NaeI 116 GCCGGC GCC^GGC
NarI 191 GGCGCC GG^CGCC
NgoMIV 116 GCCGGC G^CCGGC
PflFI 961 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 196 CTGCAG CTGCA^G
SapI 225 GCTCTTC GCTCTTC(1/4)
SexAI 1118 ACCAGGT A^CC[AT]GGT
SfcI 196 CTGCAG C^T[GA][CT]AG
SfoI 191 GGCGCC GGC^GCC
TfiI 732 GAATC G^A[AT]TC
Tru9I 758 TTAA T^TAA
Tth111I 961 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 450 TCTAGA T^CTAGA
XhoII 626 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 702 GTAC GT^AC
871 GTAC
AlwI 512 GGATC GGATC(4/5)
783 GGATC
AvaII 243 GGACC G^G[AT]CC
249 GGACC
BanI 191 GGCGCC G^G[CT][GA]CC
271 GGCACC
BpmI 79 CTGGAG CTGGAG(16/14)
789 CTGGAG
BsaI 442 GAGACC GGTCTC(1/5)
557 GAGACC
BsmAI 442 GAGAC GTCTC(1/5)
557 GAGAC
BsrBI 894 CCGCTC CCGCTC(-3/-3)
94 GAGCGG
BsrDI 436 CATTGC GCAATG(2/0)
874 CATTGC
BsrFI 116 GCCGGC [GA]^CCGG[CT]
213 GCCGGT
BssHII 219 GCGCGC G^CGCGC
221 GCGCGC
BstF5I 171 CATCC GGATG(2/0)
582 GGATG
BstNI 393 CCTGG CC^[AT]GG
1119 CCAGG
BstXI 158 CCATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
665 CCACCAACTTGG
Cfr10I 116 GCCGGC [GA]^CCGG[CT]
213 GCCGGT
EarI 226 CTCTTC CTCTTC(1/4)
525 CTCTTC
EcoRII 393 CCTGG ^CC[AT]GG
1119 CCAGG
FokI 171 CATCC GGATG(9/13)
582 GGATG
HaeII 191 GGCGCC [GA]GCGC^[CT]
263 GGCGCT
HgaI 1035 GACGC GACGC(5/10)
31 GCGTC
HphI 257 TCACC GGTGA(8/7)
716 TCACC
Ksp632I 226 CTCTTC CTCTTC(1/4)
525 CTCTTC
MboII 227 TCTTC GAAGA(8/7)
526 TCTTC
MslI 159 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
279 CACAGTGGTG
MvaI 393 CCTGG CC^[AT]GG
1119 CCAGG
RsaI 702 GTAC GT^AC
871 GTAC
SmaI 174 CCCGGG CCC^GGG
845 CCCGGG
SmlI 355 CTCAAG C^T[CT][GA]AG
364 CTCAAG
Tsp509I 689 AATT ^AATT
724 AATT
XmaCI 174 CCCGGG C^CCGGG
845 CCCGGG
XmaI 174 CCCGGG C^CCGGG
845 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaI
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeII MaeIII MboI MnlI
MspA1I MspI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I