Restriction map for
pF1KB7342
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AlwNI AocI Aor51HI AscI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsiWI
BsoBI BspDI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI Bsu36I CelII
ClaI CpoI DraI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FauI FseI FspI HaeII
HgaI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NarI NcoI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1171 GTATAC GT^[AC][GT]AC
AccIII 769 TCCGGA T^CCGGA
AciI 794 CCGC CCGC(-3/-1)
AflII 1515 CTTAAG C^TTAAG
AgeI 898 ACCGGT A^CCGGT
AhdI 1743 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1359 GTGCAC G^TGCAC
ApaI 1401 GGGCCC GGGCC^C
ApaLI 1359 GTGCAC G^TGCAC
AseI 1558 ATTAAT AT^TAAT
AsnI 1558 ATTAAT AT^TAAT
AspEI 1743 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 615 CGAACAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1474 GGATAC GTATCC(6/5)
BclI 683 TGATCA T^GATCA
BcnI 1491 CCCGG CC^[GC]GG
BfrI 1515 CTTAAG C^TTAAG
BseAI 769 TCCGGA T^CCGGA
BsgI 485 CTGCAC GTGCAG(16/14)
BsiEI 2598 CGATCG CG[GA][CT]^CG
BsmFI 2616 GGGAC GGGAC(10/14)
BsmI 1408 GAATGC GAATGC(1/-1)
Bsp1407I 586 TGTACA T^GTACA
BspEI 769 TCCGGA T^CCGGA
BspLU11I 2128 ACATGT A^CATGT
BsrGI 586 TGTACA T^GTACA
Bst1107I 1171 GTATAC GTA^TAC
BstZ17I 1171 GTATAC GTA^TAC
CfoI 390 GCGC GCG^C
DraIII 730 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2394 GACCTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1743 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 209 GAATTC G^AATTC
EcoRV 577 GATATC GAT^ATC
EcoT22I 2670 ATGCAT ATGCA^T
FbaI 683 TGATCA T^GATCA
HhaI 390 GCGC GCG^C
HinP1I 390 GCGC G^CGC
HpaI 1977 GTTAAC GTT^AAC
MroI 769 TCCGGA T^CCGGA
MspA1I 1454 CAGCTG C[AC]G^C[GT]G
NaeI 2575 GCCGGC GCC^GGC
NciI 1491 CCCGG CC^[GC]GG
NdeI 1972 CATATG CA^TATG
NgoMIV 2575 GCCGGC G^CCGGC
NsiI 2670 ATGCAT ATGCA^T
PinAI 898 ACCGGT A^CCGGT
PpuMI 2217 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1558 ATTAAT AT^TAAT
PvuI 2598 CGATCG CGAT^CG
PvuII 1454 CAGCTG CAG^CTG
SacI 1338 GAGCTC GAGCT^C
ScaI 1593 AGTACT AGT^ACT
SspBI 586 TGTACA T^GTACA
TaqI 105 TCGA T^CGA
TthHB8I 105 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 459 ACACGT A^C[GA][CT]GT
2128 ACATGT
AspHI 1338 GAGCTC G[AT]GC[AT]^C
1359 GTGCAC
BalI 1458 TGGCCA TGG^CCA
2310 TGGCCA
BsiHKAI 1338 GAGCTC G[AT]GC[AT]^C
1359 GTGCAC
BsrFI 898 ACCGGT [GA]^CCGG[CT]
2575 GCCGGC
BstAPI 640 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1651 GCAGTGGATGC
Cfr10I 898 ACCGGT [GA]^CCGG[CT]
2575 GCCGGC
EarI 360 GAAGAG CTCTTC(1/4)
2611 GAAGAG
EcoT14I 1470 CCTTGG C^C[AT][AT]GG
2396 CCTTGG
HincII 1895 GTCAAC GT[CT]^[GA]AC
1977 GTTAAC
HindII 1895 GTCAAC GT[CT]^[GA]AC
1977 GTTAAC
Ksp632I 360 GAAGAG CTCTTC(1/4)
2611 GAAGAG
MluNI 1458 TGGCCA TGG^CCA
2310 TGGCCA
MscI 1458 TGGCCA TGG^CCA
2310 TGGCCA
PstI 647 CTGCAG CTGCA^G
2592 CTGCAG
SspI 1636 AATATT AAT^ATT
2245 AATATT
StyI 1470 CCTTGG C^C[AT][AT]GG
2396 CCTTGG
XbaI 213 TCTAGA T^CTAGA
2628 TCTAGA
XcmI 1461 CCAAGAACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2469 CCAACCTCGGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII