Restriction map for
pF1KB7695
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspMI
BsrBI BsrDI BsrGI BsrI Bst1107I
BstBI BstXI BstYI BstZ17I ClaI
CpoI DraI DraIII EarI Eco47III
EcoRI EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MfeI MflI
MluI MseI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 536 TCCGGA T^CCGGA
AcyI 786 GGCGCC G[GA]^CG[CT]C
AhdI 849 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 754 CCTGAGG CC^T[ACGT]AGG
AspEI 849 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 61 GAGCAC G[AT]GC[AT]^C
AvrII 30 CCTAGG C^CTAGG
BalI 2 TGGCCA TGG^CCA
BbeI 786 GGCGCC GGCGC^C
BfaI 31 CTAG C^TAG
BlnI 30 CCTAGG C^CTAGG
BlpI 627 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 627 GCTCAGC GC^T[ACGT]AGC
BsaAI 213 CACGTA [CT]AC^GT[GA]
BsaHI 786 GGCGCC G[GA]^CG[CT]C
BseAI 536 TCCGGA T^CCGGA
BsgI 365 GTGCAG GTGCAG(16/14)
BsiHKAI 61 GAGCAC G[AT]GC[AT]^C
BsmBI 370 GAGACG CGTCTC(1/5)
BspEI 536 TCCGGA T^CCGGA
BspLU11I 1052 ACATGT A^CATGT
BssHII 82 GCGCGC G^CGCGC
BssSI 906 CTCGTG CACGAG(-5/-1)
BstEII 1045 GGTTACC G^GT[ACGT]ACC
BstPI 1045 GGTTACC G^GT[ACGT]ACC
Bsu36I 754 CCTGAGG CC^T[ACGT]AGG
CelII 627 GCTCAGC GC^T[ACGT]AGC
DrdI 793 GACGGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 619 CGGCCG C^GGCCG
Eam1105I 849 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 619 CGGCCG C^GGCCG
Eco52I 619 CGGCCG C^GGCCG
Eco81I 754 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1045 GGTTACC G^GT[ACGT]ACC
EcoRV 592 GATATC GAT^ATC
HaeII 786 GGCGCC [GA]GCGC^[CT]
Hin1I 786 GGCGCC G[GA]^CG[CT]C
KasI 786 GGCGCC G^GCGCC
KspI 180 CCGCGG CCGC^GG
MaeI 31 CTAG C^TAG
MaeII 214 ACGT A^CGT
MluNI 2 TGGCCA TGG^CCA
MroI 536 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MslI 159 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 786 GGCGCC GG^CGCC
NcoI 1073 CCATGG C^CATGG
NdeI 518 CATATG CA^TATG
NspI 1052 ACATGT [GA]CATG^[CT]
PstI 981 CTGCAG CTGCA^G
PvuI 1038 CGATCG CGAT^CG
PvuII 1015 CAGCTG CAG^CTG
SacII 180 CCGCGG CCGC^GG
SfcI 981 CTGCAG C^T[GA][CT]AG
SfoI 786 GGCGCC GGC^GCC
SmaI 198 CCCGGG CCC^GGG
XmaCI 198 CCCGGG C^CCGGG
XmaI 198 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 212 ACACGT A^C[GA][CT]GT
1052 ACATGT
BanI 786 GGCGCC G^G[CT][GA]CC
1121 GGCACC
BglI 778 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
830 GCCTGGGGGGC
BsaI 406 GAGACC GGTCTC(1/5)
532 GGTCTC
BsaWI 536 TCCGGA [AT]^CCGG[AT]
707 ACCGGA
BsiEI 619 CGGCCG CG[GA][CT]^CG
1038 CGATCG
DsaI 180 CCGCGG C^C[GA][CT]GG
1073 CCATGG
EcoT14I 30 CCTAGG C^C[AT][AT]GG
1073 CCATGG
PaeR7I 349 CTCGAG C^TCGAG
457 CTCGAG
SfaNI 524 GCATC GCATC(5/9)
965 GCATC
SmlI 349 CTCGAG C^T[CT][GA]AG
457 CTCGAG
StyI 30 CCTAGG C^C[AT][AT]GG
1073 CCATGG
Tsp45I 821 GTGAC ^GT[GC]AC
1106 GTCAC
Tsp509I 740 AATT ^AATT
917 AATT
XhoI 349 CTCGAG C^TCGAG
457 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI TaqI
TseI TspRI TthHB8I