Restriction map for
pF1KE0136
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Cfr10I CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 361 GACGTC GACGT^C
AccII 682 CGCG CG^CG
AcyI 361 GACGTC G[GA]^CG[CT]C
AflIII 341 ACGTGT A^C[GA][CT]GT
AlwI 531 GATCC GGATC(4/5)
AocI 451 CCTAAGG CC^T[ACGT]AGG
Aor51HI 701 AGCGCT AGC^GCT
AspI 679 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 371 CCCGGG C^[CT]CG[GA]G
BbvCI 886 CCTCAGC CCTCAGC(-5/-2)
BciVI 134 GGATAC GTATCC(6/5)
BlpI 819 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 819 GCTGAGC GC^T[ACGT]AGC
BsaAI 340 TACGTG [CT]AC^GT[GA]
BsaHI 361 GACGTC G[GA]^CG[CT]C
BsaI 501 GGTCTC GGTCTC(1/5)
BsmBI 289 GAGACG CGTCTC(1/5)
BsoBI 371 CCCGGG C^[CT]CG[GA]G
Bsp1407I 344 TGTACA T^GTACA
BspDI 221 ATCGAT AT^CGAT
BspMI 111 GCAGGT ACCTGC(4/8)
BsrDI 407 GCAATG GCAATG(2/0)
BsrGI 344 TGTACA T^GTACA
BssSI 747 CACGAG CACGAG(-5/-1)
BstAPI 662 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 682 CGCG CG^CG
Bsu36I 451 CCTAAGG CC^T[ACGT]AGG
CelII 819 GCTGAGC GC^T[ACGT]AGC
ClaI 221 ATCGAT AT^CGAT
DpnII 531 GATC ^GATC
DraIII 619 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 800 CCGTGG C^C[GA][CT]GG
Eco47III 701 AGCGCT AGC^GCT
Eco81I 451 CCTAAGG CC^T[ACGT]AGG
EcoT14I 95 CCAAGG C^C[AT][AT]GG
Hin1I 361 GACGTC G[GA]^CG[CT]C
MboI 531 GATC ^GATC
MvnI 682 CGCG CG^CG
NdeII 531 GATC ^GATC
PflFI 679 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 326 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 163 CTGCAG CTGCA^G
Sau3AI 531 GATC ^GATC
SfaNI 59 GCATC GCATC(5/9)
SmaI 371 CCCGGG CCC^GGG
SspBI 344 TGTACA T^GTACA
StuI 50 AGGCCT AGG^CCT
StyI 95 CCAAGG C^C[AT][AT]GG
Tth111I 679 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 371 CCCGGG C^CCGGG
XmaI 371 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 300 GTAC GT^AC
345 GTAC
AlwNI 79 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
325 CAGGTCCTG
ApaI 44 GGGCCC GGGCC^C
238 GGGCCC
AvaII 327 GGTCC G^G[AT]CC
432 GGTCC
BanII 44 GGGCCC G[GA]GC[CT]^C
238 GGGCCC
BcnI 371 CCCGG CC^[GC]GG
372 CCGGG
BglI 37 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
94 GCCAAGGTGGC
BpmI 496 CTGGAG CTGGAG(16/14)
592 CTGGAG
BsmAI 289 GAGAC GTCTC(1/5)
502 GTCTC
EarI 22 CTCTTC CTCTTC(1/4)
180 CTCTTC
FauI 435 CCCGC CCCGC(4/6)
654 GCGGG
HgaI 683 GCGTC GACGC(5/10)
836 GCGTC
HinfI 178 GACTC G^A[ACGT]TC
711 GAGTC
Ksp632I 22 CTCTTC CTCTTC(1/4)
180 CTCTTC
MboII 23 TCTTC GAAGA(8/7)
181 TCTTC
NciI 371 CCCGG CC^[GC]GG
372 CCGGG
NlaIII 156 CATG CATG^
285 CATG
PleI 178 GACTC GAGTC(4/5)
711 GAGTC
PvuII 265 CAGCTG CAG^CTG
889 CAGCTG
RsaI 300 GTAC GT^AC
345 GTAC
SfcI 163 CTGCAG C^T[GA][CT]AG
243 CTACAG
SfiI 36 GGCCAATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
93 GGCCAAGGTGGCC
TaqI 210 TCGA T^CGA
222 TCGA
TthHB8I 210 TCGA T^CGA
222 TCGA
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI BalI BbvI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NlaIV
Sau96I ScrFI TseI Tsp45I TspRI
XcmI