Restriction map for
pF1KE0511
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanII BbeI
BbrPI BcgI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmAI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI KspI
MaeII MfeI MluI MroI MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 909 GTCTAC GT^[AC][GT]AC
AfaI 255 GTAC GT^AC
AflIII 765 ACATGT A^C[GA][CT]GT
AocI 46 CCTGAGG CC^T[ACGT]AGG
AseI 595 ATTAAT AT^TAAT
AsnI 595 ATTAAT AT^TAAT
Asp700I 295 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 351 GGACC G^G[AT]CC
BalI 680 TGGCCA TGG^CCA
BamHI 312 GGATCC G^GATCC
BanI 89 GGCACC G^G[CT][GA]CC
BbsI 337 GAAGAC GAAGAC(2/6)
BbvCI 148 GCTGAGG CCTCAGC(-5/-2)
BbvI 710 GCAGC GCAGC(8/12)
BciVI 403 GGATAC GTATCC(6/5)
BclI 621 TGATCA T^GATCA
BfaI 22 CTAG C^TAG
BpmI 714 CTGGAG CTGGAG(16/14)
BpuAI 337 GAAGAC GAAGAC(2/6)
BsaJI 518 CCCTGG C^C[ACGT][ACGT]GG
BsmFI 185 GGGAC GGGAC(10/14)
BsmI 706 GAATGC GAATGC(1/-1)
BspHI 303 TCATGA T^CATGA
BspLU11I 765 ACATGT A^CATGT
BsrI 422 CCAGT ACTGG(1/-1)
BstEII 816 GGTTACC G^GT[ACGT]ACC
BstF5I 539 GGATG GGATG(2/0)
BstPI 816 GGTTACC G^GT[ACGT]ACC
Bsu36I 46 CCTGAGG CC^T[ACGT]AGG
Cfr13I 351 GGACC G^G[ACGT]CC
DraI 289 TTTAAA TTT^AAA
DraII 350 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 680 TGGCCA [CT]^GGCC[GA]
Eco81I 46 CCTGAGG CC^T[ACGT]AGG
EcoO109I 350 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 816 GGTTACC G^GT[ACGT]ACC
FbaI 621 TGATCA T^GATCA
Fnu4HI 710 GCAGC GC^[ACGT]GC
FokI 539 GGATG GGATG(9/13)
HincII 654 GTTGAC GT[CT]^[GA]AC
HindII 654 GTTGAC GT[CT]^[GA]AC
HindIII 62 AAGCTT A^AGCTT
ItaI 710 GCAGC GC^[ACGT]GC
MaeI 22 CTAG C^TAG
MluNI 680 TGGCCA TGG^CCA
MscI 680 TGGCCA TGG^CCA
MspA1I 711 CAGCTG C[AC]G^C[GT]G
NdeI 455 CATATG CA^TATG
NspI 765 ACATGT [GA]CATG^[CT]
PpuMI 350 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 595 ATTAAT AT^TAAT
PvuII 711 CAGCTG CAG^CTG
RcaI 303 TCATGA T^CATGA
RsaI 255 GTAC GT^AC
Sau96I 351 GGACC G^G[ACGT]CC
SfaNI 397 GATGC GCATC(5/9)
SmlI 346 CTTGAG C^T[CT][GA]AG
SspI 592 AATATT AAT^ATT
StuI 498 AGGCCT AGG^CCT
TseI 710 GCAGC G^C[AT]GC
XmnI 295 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BsaBI 97 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
467 GATATACATC
BssKI 367 CCAGG ^CC[ACGT]GG
519 CCTGG
BstNI 367 CCAGG CC^[AT]GG
519 CCTGG
BstYI 312 GGATCC [GA]^GATC[CT]
831 AGATCC
EarI 513 CTCTTC CTCTTC(1/4)
688 GAAGAG
EcoRII 367 CCAGG ^CC[AT]GG
519 CCTGG
HaeIII 499 GGCC GG^CC
681 GGCC
HinfI 340 GACTC G^A[ACGT]TC
854 GAGTC
HphI 43 TCACC GGTGA(8/7)
742 TCACC
Ksp632I 513 CTCTTC CTCTTC(1/4)
688 GAAGAG
MaeIII 559 GTTAC ^GT[ACGT]AC
817 GTTAC
MamI 97 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
467 GATATACATC
MflI 312 GGATCC [GA]^GATC[CT]
831 AGATCC
MslI 80 CACTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
235 CATTTTGATG
MvaI 367 CCAGG CC^[AT]GG
519 CCTGG
NlaIV 89 GGCACC GG[ACGT]^[ACGT]CC
312 GGATCC
PleI 340 GACTC GAGTC(4/5)
854 GAGTC
ScrFI 367 CCAGG CC^[ACGT]GG
519 CCTGG
TspRI 417 CACTG CA[GC]TG[ACGT][ACGT]^
423 CAGTG
XhoII 312 GGATCC [GA]^GATC[CT]
831 AGATCC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AcsI AluI AlwI ApoI DdeI
DpnII MboI MboII MnlI MseI
NdeII NlaIII Sau3AI Tru9I Tsp509I