Restriction map for
pF1KE0429
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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Acc65I 665 GGTACC G^GTACC
Alw44I 728 GTGCAC G^TGCAC
ApaLI 728 GTGCAC G^TGCAC
Asp718I 665 GGTACC G^GTACC
BbsI 682 GAAGAC GAAGAC(2/6)
BbvCI 1207 CCTCAGC CCTCAGC(-5/-2)
BclI 168 TGATCA T^GATCA
BglI 615 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 682 GAAGAC GAAGAC(2/6)
BsgI 345 CTGCAC GTGCAG(16/14)
BssSI 1272 CTCGTG CACGAG(-5/-1)
CfoI 717 GCGC GCG^C
DraIII 504 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 383 CCACGG C^C[GA][CT]GG
FbaI 168 TGATCA T^GATCA
HhaI 717 GCGC GCG^C
HinP1I 717 GCGC G^CGC
KpnI 665 GGTACC GGTAC^C
MaeII 1159 ACGT A^CGT
PshAI 70 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 339 GAGCTC GAGCT^C
SmlI 103 CTCAAG C^T[CT][GA]AG
XcmI 153 CCAAGAGAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 593 GTAC GT^AC
666 GTAC
AlwI 988 GATCC GGATC(4/5)
1250 GATCC
AocI 481 CCTCAGG CC^T[ACGT]AGG
552 CCTCAGG
AspHI 339 GAGCTC G[AT]GC[AT]^C
728 GTGCAC
BanI 665 GGTACC G^G[CT][GA]CC
1227 GGTGCC
BfaI 146 CTAG C^TAG
584 CTAG
BpmI 366 CTCCAG CTGGAG(16/14)
1174 CTGGAG
BsiHKAI 339 GAGCTC G[AT]GC[AT]^C
728 GTGCAC
BsmAI 862 GAGAC GTCTC(1/5)
490 GTCTC
BspMI 735 GCAGGT ACCTGC(4/8)
1224 GCAGGT
BsrBI 1140 CCGCTC CCGCTC(-3/-3)
111 GAGCGG
BstEII 189 GGTCACC G^GT[ACGT]ACC
1032 GGTCACC
BstPI 189 GGTCACC G^GT[ACGT]ACC
1032 GGTCACC
BstYI 987 AGATCC [GA]^GATC[CT]
1249 AGATCC
Bsu36I 481 CCTCAGG CC^T[ACGT]AGG
552 CCTCAGG
DrdI 142 GACCCTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
864 GACACACAAGTC
EarI 492 CTCTTC CTCTTC(1/4)
1005 CTCTTC
Eco81I 481 CCTCAGG CC^T[ACGT]AGG
552 CCTCAGG
EcoO65I 189 GGTCACC G^GT[ACGT]ACC
1032 GGTCACC
Ksp632I 492 CTCTTC CTCTTC(1/4)
1005 CTCTTC
MaeI 146 CTAG C^TAG
584 CTAG
MflI 987 AGATCC [GA]^GATC[CT]
1249 AGATCC
RsaI 593 GTAC GT^AC
666 GTAC
SmaI 256 CCCGGG CCC^GGG
262 CCCGGG
XhoII 987 AGATCC [GA]^GATC[CT]
1249 AGATCC
XmaCI 256 CCCGGG C^CCGGG
262 CCCGGG
XmaI 256 CCCGGG C^CCGGG
262 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI ApaI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StuI TfiI
TseI Tsp45I TspRI