Restriction map for
pF1KB8579
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI Eco47III EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 442 GACGTC GACGT^C
AfaI 663 GTAC GT^AC
AhdI 157 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 564 GATCC GGATC(4/5)
AocI 259 CCTGAGG CC^T[ACGT]AGG
AspEI 157 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 113 CCCGGG C^[CT]CG[GA]G
AvaII 15 GGACC G^G[AT]CC
BanI 507 GGTGCC G^G[CT][GA]CC
BbsI 202 GAAGAC GAAGAC(2/6)
BglI 374 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 145 CTGGAG CTGGAG(16/14)
BpuAI 202 GAAGAC GAAGAC(2/6)
BsiEI 82 CGGCCG CG[GA][CT]^CG
BsoBI 113 CCCGGG C^[CT]CG[GA]G
Bsp1407I 662 TGTACA T^GTACA
BsrBI 132 CCGCTC CCGCTC(-3/-3)
BsrGI 662 TGTACA T^GTACA
BssHII 343 GCGCGC G^CGCGC
BstXI 137 CCACGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 563 AGATCC [GA]^GATC[CT]
Bsu36I 259 CCTGAGG CC^T[ACGT]AGG
DraIII 472 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 82 CGGCCG [CT]^GGCC[GA]
EagI 82 CGGCCG C^GGCCG
Eam1105I 157 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 42 CTCTTC CTCTTC(1/4)
EclXI 82 CGGCCG C^GGCCG
Eco52I 82 CGGCCG C^GGCCG
Eco81I 259 CCTGAGG CC^T[ACGT]AGG
EcoNI 309 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 426 GCGGG CCCGC(4/6)
HaeII 432 GGCGCT [GA]GCGC^[CT]
Ksp632I 42 CTCTTC CTCTTC(1/4)
MaeII 443 ACGT A^CGT
MaeIII 254 GTGAC ^GT[ACGT]AC
MflI 563 AGATCC [GA]^GATC[CT]
MslI 271 CACTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 471 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 298 CTGCAG CTGCA^G
RsaI 663 GTAC GT^AC
SacI 150 GAGCTC GAGCT^C
SmaI 113 CCCGGG CCC^GGG
SspBI 662 TGTACA T^GTACA
Tsp45I 254 GTGAC ^GT[GC]AC
Van91I 471 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 563 AGATCC [GA]^GATC[CT]
XmaCI 113 CCCGGG C^CCGGG
XmaI 113 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 335 GACGCC G[GA]^CG[CT]C
442 GACGTC
AspHI 150 GAGCTC G[AT]GC[AT]^C
630 GAGCAC
BanII 150 GAGCTC G[GA]GC[CT]^C
327 GGGCTC
BcnI 113 CCCGG CC^[GC]GG
114 CCGGG
BfaI 5 CTAG C^TAG
359 CTAG
BlpI 92 GCTCAGC GC^T[ACGT]AGC
627 GCTGAGC
Bpu1102I 92 GCTCAGC GC^T[ACGT]AGC
627 GCTGAGC
BsaHI 335 GACGCC G[GA]^CG[CT]C
442 GACGTC
BsiHKAI 150 GAGCTC G[AT]GC[AT]^C
630 GAGCAC
BssSI 138 CACGAG CACGAG(-5/-1)
529 CTCGTG
BstAPI 291 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
461 GCAAGAGCTGC
BstF5I 219 GGATG GGATG(2/0)
264 GGATG
CelII 92 GCTCAGC GC^T[ACGT]AGC
627 GCTGAGC
DpnII 79 GATC ^GATC
564 GATC
FokI 219 GGATG GGATG(9/13)
264 GGATG
HapII 114 CCGG C^CGG
196 CCGG
HgaI 335 GACGC GACGC(5/10)
624 GACGC
Hin1I 335 GACGCC G[GA]^CG[CT]C
442 GACGTC
HpaII 114 CCGG C^CGG
196 CCGG
MaeI 5 CTAG C^TAG
359 CTAG
MboI 79 GATC ^GATC
564 GATC
MspI 114 CCGG C^CGG
196 CCGG
NciI 113 CCCGG CC^[GC]GG
114 CCGGG
NdeII 79 GATC ^GATC
564 GATC
NlaIV 14 GGGACC GG[ACGT]^[ACGT]CC
507 GGTGCC
Sau3AI 79 GATC ^GATC
564 GATC
SfaNI 288 GATGC GCATC(5/9)
354 GATGC
SfcI 298 CTGCAG C^T[GA][CT]AG
405 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BmyI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EcoRII Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI MboII MnlI MspA1I
MvaI MvnI MwoI PleI PvuII
Sau96I ScrFI TaqI TseI TspRI
TthHB8I