Restriction map for
pF1KE0508
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BpuAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cac8I CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MluI
MluNI MroI MscI MseI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 35 ACGTGT A^C[GA][CT]GT
Alw44I 127 GTGCAC G^TGCAC
AlwI 456 GATCC GGATC(4/5)
ApaLI 127 GTGCAC G^TGCAC
Asp700I 244 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 177 CCCGGG C^[CT]CG[GA]G
BanII 474 GAGCTC G[GA]GC[CT]^C
BbvI 170 GCTGC GCAGC(8/12)
BlpI 69 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 69 GCTCAGC GC^T[ACGT]AGC
BsaAI 34 TACGTG [CT]AC^GT[GA]
BsaWI 21 TCCGGT [AT]^CCGG[AT]
BseRI 264 CTCCTC GAGGAG(10/8)
BsoBI 177 CCCGGG C^[CT]CG[GA]G
BspMI 389 ACCTGC ACCTGC(4/8)
BsrDI 188 CATTGC GCAATG(2/0)
BstYI 455 AGATCC [GA]^GATC[CT]
CelII 69 GCTCAGC GC^T[ACGT]AGC
DdeI 70 CTCAG C^T[ACGT]AG
DpnII 456 GATC ^GATC
DsaI 479 CCGTGG C^C[GA][CT]GG
EaeI 495 CGGCCA [CT]^GGCC[GA]
EcoNI 463 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII 35 ACGT A^CGT
MboI 456 GATC ^GATC
MfeI 318 CAATTG C^AATTG
MflI 455 AGATCC [GA]^GATC[CT]
MspA1I 168 CAGCTG C[AC]G^C[GT]G
MunI 318 CAATTG C^AATTG
MwoI 476 GCTCCGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 456 GATC ^GATC
NlaIII 81 CATG CATG^
PvuII 168 CAGCTG CAG^CTG
SacI 474 GAGCTC GAGCT^C
Sau3AI 456 GATC ^GATC
SfaNI 104 GCATC GCATC(5/9)
SmaI 177 CCCGGG CCC^GGG
TseI 170 GCTGC G^C[AT]GC
Tsp45I 235 GTCAC ^GT[GC]AC
Tsp509I 319 AATT ^AATT
TspRI 153 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 455 AGATCC [GA]^GATC[CT]
XmaCI 177 CCCGGG C^CCGGG
XmaI 177 CCCGGG C^CCGGG
XmnI 244 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwNI 203 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
349 CAGTTCCTG
AspHI 127 GTGCAC G[AT]GC[AT]^C
474 GAGCTC
BmyI 127 GTGCAC G[AGT]GC[ACT]^C
474 GAGCTC
BsiHKAI 127 GTGCAC G[AT]GC[AT]^C
474 GAGCTC
BsmFI 184 GGGAC GGGAC(10/14)
279 GTCCC
Bsp1286I 127 GTGCAC G[AGT]GC[ACT]^C
474 GAGCTC
BsrI 96 ACTGG ACTGG(1/-1)
358 ACTGG
BstF5I 116 CATCC GGATG(2/0)
135 GGATG
BstXI 50 CCAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
77 CCATCATGCTGG
FokI 116 CATCC GGATG(9/13)
135 GGATG
HphI 24 GGTGA GGTGA(8/7)
292 GGTGA
MaeIII 235 GTCAC ^GT[ACGT]AC
323 GTAAC
MslI 120 CATAGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
130 CACAAGGATG
NlaIV 3 GGAGCC GG[ACGT]^[ACGT]CC
434 GGCCCC
StuI 54 AGGCCT AGG^CCT
296 AGGCCT
TfiI 12 GAATC G^A[AT]TC
369 GATTC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AluI BcnI BpmI
BsaJI BsiYI BslI BssKI BstNI
Cfr13I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI MboII MnlI
MspI MvaI NciI PleI RsaI
Sau96I ScrFI