Restriction map for
pF1KE0209
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsiWI BsmBI BsmI
BsoBI BspDI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HinfI HpaI KpnI Ksp632I KspI
MaeI MfeI MluI MluNI MscI
MslI MunI NaeI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 853 GGCGCC G[GA]^CG[CT]C
Aor51HI 295 AGCGCT AGC^GCT
ApaI 278 GGGCCC GGGCC^C
AspHI 880 GAGCAC G[AT]GC[AT]^C
BanI 853 GGCGCC G^G[CT][GA]CC
BanII 278 GGGCCC G[GA]GC[CT]^C
BbeI 853 GGCGCC GGCGC^C
BbvCI 816 GCTGAGG CCTCAGC(-5/-2)
BglI 15 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 561 CTGGAG CTGGAG(16/14)
BsaBI 312 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 853 GGCGCC G[GA]^CG[CT]C
BsaI 454 GAGACC GGTCTC(1/5)
BseRI 877 GAGGAG GAGGAG(10/8)
BsgI 358 GTGCAG GTGCAG(16/14)
BsiEI 25 CGGCCG CG[GA][CT]^CG
BsiHKAI 880 GAGCAC G[AT]GC[AT]^C
Bsp1407I 886 TGTACA T^GTACA
BspHI 159 TCATGA T^CATGA
BsrFI 101 ACCGGC [GA]^CCGG[CT]
BsrGI 886 TGTACA T^GTACA
BssSI 40 CTCGTG CACGAG(-5/-1)
BstXI 293 CCAGCGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 180 GGATCT [GA]^GATC[CT]
Cfr10I 101 ACCGGC [GA]^CCGG[CT]
DraII 896 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 796 CCATGG C^C[GA][CT]GG
EaeI 25 CGGCCG [CT]^GGCC[GA]
EagI 25 CGGCCG C^GGCCG
EclXI 25 CGGCCG C^GGCCG
Eco47III 295 AGCGCT AGC^GCT
Eco52I 25 CGGCCG C^GGCCG
EcoO109I 896 GGGACCC [GA]G^G[ACGT]CC[CT]
Hin1I 853 GGCGCC G[GA]^CG[CT]C
HincII 220 GTCAAC GT[CT]^[GA]AC
HindII 220 GTCAAC GT[CT]^[GA]AC
KasI 853 GGCGCC G^GCGCC
MaeII 365 ACGT A^CGT
MamI 312 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 600 GAAGA GAAGA(8/7)
MflI 180 GGATCT [GA]^GATC[CT]
MseI 500 TTAA T^TAA
NarI 853 GGCGCC GG^CGCC
NcoI 796 CCATGG C^CATGG
NotI 24 GCGGCCGC GC^GGCCGC
NspI 514 ACATGC [GA]CATG^[CT]
PflMI 188 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 896 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 636 CAGCTG CAG^CTG
RcaI 159 TCATGA T^CATGA
ScaI 764 AGTACT AGT^ACT
SfoI 853 GGCGCC GGC^GCC
SspBI 886 TGTACA T^GTACA
Tru9I 500 TTAA T^TAA
Van91I 188 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 180 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 10 CGCG CG^CG
57 CGCG
AccIII 545 TCCGGA T^CCGGA
776 TCCGGA
AcsI 523 GAATTC [GA]^AATT[CT]
780 GAATTC
AluI 193 AGCT AG^CT
637 AGCT
ApoI 523 GAATTC [GA]^AATT[CT]
780 GAATTC
BmyI 278 GGGCCC G[AGT]GC[ACT]^C
880 GAGCAC
BseAI 545 TCCGGA T^CCGGA
776 TCCGGA
BsmAI 454 GAGAC GTCTC(1/5)
572 GAGAC
Bsp1286I 278 GGGCCC G[AGT]GC[ACT]^C
880 GAGCAC
BspEI 545 TCCGGA T^CCGGA
776 TCCGGA
BspMI 94 ACCTGC ACCTGC(4/8)
457 ACCTGC
BsrDI 206 GCAATG GCAATG(2/0)
518 GCAATG
BstUI 10 CGCG CG^CG
57 CGCG
DdeI 761 CTGAG C^T[ACGT]AG
817 CTGAG
EcoRI 523 GAATTC G^AATTC
780 GAATTC
EcoT14I 476 CCAAGG C^C[AT][AT]GG
796 CCATGG
HaeII 295 AGCGCT [GA]GCGC^[CT]
853 GGCGCC
HphI 790 GGTGA GGTGA(8/7)
236 TCACC
MroI 545 TCCGGA T^CCGGA
776 TCCGGA
MvnI 10 CGCG CG^CG
57 CGCG
StyI 476 CCAAGG C^C[AT][AT]GG
796 CCATGG
TaqI 371 TCGA T^CGA
875 TCGA
Tsp45I 491 GTGAC ^GT[GC]AC
826 GTGAC
Tsp509I 524 AATT ^AATT
781 AATT
TspRI 750 CAGTG CA[GC]TG[ACGT][ACGT]^
883 CACTG
TthHB8I 371 TCGA T^CGA
875 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AlwI AvaII BbvI
BcnI BsaJI BsaWI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI