Restriction map for
pF1KE0495
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaHI BsaI BseAI BseRI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 231 GTAC GT^AC
AvaI 345 CTCGAG C^[CT]CG[GA]G
BamHI 611 GGATCC G^GATCC
BanII 570 GGGCTC G[GA]GC[CT]^C
BbvI 225 GCAGC GCAGC(8/12)
BlpI 396 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC
BsaBI 403 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 541 TCCGGT [AT]^CCGG[AT]
BsgI 595 GTGCAG GTGCAG(16/14)
BsiEI 122 CGGCCG CG[GA][CT]^CG
BsmAI 157 GAGAC GTCTC(1/5)
BsmFI 60 GGGAC GGGAC(10/14)
BsmI 526 GAATGC GAATGC(1/-1)
BsoBI 345 CTCGAG C^[CT]CG[GA]G
BsrFI 104 GCCGGC [GA]^CCGG[CT]
BsrI 489 CCAGT ACTGG(1/-1)
BssSI 112 CACGAG CACGAG(-5/-1)
BstXI 134 CCAGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 611 GGATCC [GA]^GATC[CT]
CelII 396 GCTGAGC GC^T[ACGT]AGC
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
DsaI 425 CCATGG C^C[GA][CT]GG
EaeI 122 CGGCCG [CT]^GGCC[GA]
EagI 122 CGGCCG C^GGCCG
EclXI 122 CGGCCG C^GGCCG
Eco52I 122 CGGCCG C^GGCCG
HinfI 343 GACTC G^A[ACGT]TC
MaeII 461 ACGT A^CGT
MamI 403 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 611 GGATCC [GA]^GATC[CT]
MslI 538 CATTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 56 CAGCGG C[AC]G^C[GT]G
NaeI 104 GCCGGC GCC^GGC
NcoI 425 CCATGG C^CATGG
NgoMIV 104 GCCGGC G^CCGGC
NspI 207 GCATGC [GA]CATG^[CT]
PaeR7I 345 CTCGAG C^TCGAG
PleI 343 GACTC GAGTC(4/5)
RsaI 231 GTAC GT^AC
SmlI 345 CTCGAG C^T[CT][GA]AG
SphI 207 GCATGC GCATG^C
TseI 225 GCAGC G^C[AT]GC
XcmI 459 CCACGTCCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 345 CTCGAG C^TCGAG
XhoII 611 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 612 GATCC GGATC(4/5)
611 GGATC
AvaII 61 GGACC G^G[AT]CC
452 GGTCC
BcnI 496 CCGGG CC^[GC]GG
561 CCGGG
BmyI 129 GTGCCC G[AGT]GC[ACT]^C
570 GGGCTC
BpmI 418 CTCCAG CTGGAG(16/14)
142 CTGGAG
Bsp1286I 129 GTGCCC G[AGT]GC[ACT]^C
570 GGGCTC
BsrDI 42 GCAATG GCAATG(2/0)
440 GCAATG
DdeI 181 CTGAG C^T[ACGT]AG
397 CTGAG
EcoT14I 219 CCAAGG C^C[AT][AT]GG
425 CCATGG
FauI 292 CCCGC CCCGC(4/6)
58 GCGGG
MaeIII 341 GTGAC ^GT[ACGT]AC
545 GTGAC
MboII 69 GAAGA GAAGA(8/7)
359 TCTTC
NciI 496 CCGGG CC^[GC]GG
561 CCGGG
PflMI 420 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
425 CCATGGGATGG
PpuMI 60 GGGACCC [GA]G^G[AT]CC[CT]
451 GGGTCCT
SfaNI 265 GATGC GCATC(5/9)
602 GCATC
StyI 219 CCAAGG C^C[AT][AT]GG
425 CCATGG
Tsp45I 341 GTGAC ^GT[GC]AC
545 GTGAC
Van91I 420 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
425 CCATGGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TaqI TthHB8I