Restriction map for
pF1KE0001
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AclI AcsI AcyI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BbeI BcgI
BciVI BclI BfrI BlnI BsaBI
BsaHI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoT22I FbaI FspI
Hin1I HpaI KasI Ksp632I KspI
MamI MfeI MluI MluNI MscI
MseI MunI NarI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 914 GGTACC G^GTACC
AccI 580 GTCTAC GT^[AC][GT]AC
AccIII 1469 TCCGGA T^CCGGA
AflIII 904 ACGTGT A^C[GA][CT]GT
AlwNI 334 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 214 CCTCAGG CC^T[ACGT]AGG
ApaI 304 GGGCCC GGGCC^C
Asp718I 914 GGTACC G^GTACC
BanI 914 GGTACC G^G[CT][GA]CC
BbrPI 903 CACGTG CAC^GTG
BbsI 1395 GAAGAC GAAGAC(2/6)
BbvCI 1107 CCTCAGC CCTCAGC(-5/-2)
BfaI 986 CTAG C^TAG
BglI 923 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 953 AGATCT A^GATCT
BpmI 256 CTGGAG CTGGAG(16/14)
BpuAI 1395 GAAGAC GAAGAC(2/6)
BsaI 810 GAGACC GGTCTC(1/5)
BseAI 1469 TCCGGA T^CCGGA
BsiEI 1178 CGACCG CG[GA][CT]^CG
BspEI 1469 TCCGGA T^CCGGA
BsrFI 644 GCCGGC [GA]^CCGG[CT]
BstXI 311 CCAGTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 214 CCTCAGG CC^T[ACGT]AGG
Cfr10I 644 GCCGGC [GA]^CCGG[CT]
DraIII 1093 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1382 CCGTGG C^C[GA][CT]GG
EaeI 642 TGGCCG [CT]^GGCC[GA]
Eco81I 214 CCTCAGG CC^T[ACGT]AGG
EcoRV 1045 GATATC GAT^ATC
FauI 1336 GCGGG CCCGC(4/6)
FseI 643 GGCCGGCC GGCCGG^CC
HaeII 343 GGCGCT [GA]GCGC^[CT]
HgaI 8 GCGTC GACGC(5/10)
HincII 841 GTCAAC GT[CT]^[GA]AC
HindII 841 GTCAAC GT[CT]^[GA]AC
HindIII 193 AAGCTT A^AGCTT
KpnI 914 GGTACC GGTAC^C
MaeI 986 CTAG C^TAG
MroI 1469 TCCGGA T^CCGGA
MslI 873 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 644 GCCGGC GCC^GGC
NdeI 990 CATATG CA^TATG
NgoMIV 644 GCCGGC G^CCGGC
PmaCI 903 CACGTG CAC^GTG
PmlI 903 CACGTG CAC^GTG
PpuMI 1064 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 852 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 134 CCCGGG CCC^GGG
SmlI 1308 CTCAAG C^T[CT][GA]AG
TfiI 1229 GAATC G^A[AT]TC
Tsp509I 595 AATT ^AATT
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 234 GTAC GT^AC
915 GTAC
Alw44I 474 GTGCAC G^TGCAC
1330 GTGCAC
ApaLI 474 GTGCAC G^TGCAC
1330 GTGCAC
BamHI 55 GGATCC G^GATCC
1016 GGATCC
BlpI 102 GCTCAGC GC^T[ACGT]AGC
1186 GCTCAGC
Bpu1102I 102 GCTCAGC GC^T[ACGT]AGC
1186 GCTCAGC
BsaAI 903 CACGTG [CT]AC^GT[GA]
1402 TACGTG
BsaWI 1275 TCCGGT [AT]^CCGG[AT]
1469 TCCGGA
BspMI 535 ACCTGC ACCTGC(4/8)
356 GCAGGT
CelII 102 GCTCAGC GC^T[ACGT]AGC
1186 GCTCAGC
CfoI 344 GCGC GCG^C
998 GCGC
DraII 465 GGGGCCT [GA]G^G[ACGT]CC[CT]
1064 AGGTCCC
EcoO109I 465 GGGGCCT [GA]G^G[ACGT]CC[CT]
1064 AGGTCCC
EcoT14I 29 CCTTGG C^C[AT][AT]GG
60 CCAAGG
HhaI 344 GCGC GCG^C
998 GCGC
HinP1I 344 GCGC G^CGC
998 GCGC
MspA1I 681 CAGCTG C[AC]G^C[GT]G
1189 CAGCTG
PstI 269 CTGCAG CTGCA^G
684 CTGCAG
PvuII 681 CAGCTG CAG^CTG
1189 CAGCTG
RsaI 234 GTAC GT^AC
915 GTAC
SfcI 269 CTGCAG C^T[GA][CT]AG
684 CTGCAG
StyI 29 CCTTGG C^C[AT][AT]GG
60 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StuI
TseI Tsp45I TspRI XcmI XhoII