Restriction map for
pF1KE0140
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BstBI
BstEII BstPI BstXI BstYI Bsu36I
CelII ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 472 GTATAC GT^[AC][GT]AC
AccII 23 CGCG CG^CG
AfaI 633 GTAC GT^AC
AflII 492 CTTAAG C^TTAAG
AgeI 344 ACCGGT A^CCGGT
AlwI 669 GGATC GGATC(4/5)
AlwNI 512 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 821 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 730 GAGCAC G[AT]GC[AT]^C
AvaI 59 CCCGAG C^[CT]CG[GA]G
BanII 139 GAGCCC G[GA]GC[CT]^C
BfrI 492 CTTAAG C^TTAAG
BglI 551 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI 22 CCGCGG C^C[ACGT][ACGT]GG
BsaWI 344 ACCGGT [AT]^CCGG[AT]
BseRI 106 GAGGAG GAGGAG(10/8)
BsgI 365 CTGCAC GTGCAG(16/14)
BsiHKAI 730 GAGCAC G[AT]GC[AT]^C
BsmAI 643 GAGAC GTCTC(1/5)
BsoBI 59 CCCGAG C^[CT]CG[GA]G
BsrI 696 ACTGG ACTGG(1/-1)
BssSI 383 CACGAG CACGAG(-5/-1)
Bst1107I 472 GTATAC GTA^TAC
BstAPI 367 GCACATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 160 GGATG GGATG(2/0)
BstUI 23 CGCG CG^CG
BstZ17I 472 GTATAC GTA^TAC
CfoI 408 GCGC GCG^C
DpnII 670 GATC ^GATC
DraII 215 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 368 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 22 CCGCGG C^C[GA][CT]GG
EarI 700 GAAGAG CTCTTC(1/4)
EcoNI 601 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 215 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 160 GGATG GGATG(9/13)
HaeII 407 GGCGCT [GA]GCGC^[CT]
HgaI 111 GCGTC GACGC(5/10)
HhaI 408 GCGC GCG^C
HinP1I 408 GCGC G^CGC
HphI 640 GGTGA GGTGA(8/7)
Ksp632I 700 GAAGAG CTCTTC(1/4)
KspI 22 CCGCGG CCGC^GG
MboI 670 GATC ^GATC
MseI 493 TTAA T^TAA
MspA1I 22 CCGCGG C[AC]G^C[GT]G
MvnI 23 CGCG CG^CG
NdeII 670 GATC ^GATC
PinAI 344 ACCGGT A^CCGGT
PleI 62 GAGTC GAGTC(4/5)
PpuMI 215 AGGACCT [GA]G^G[AT]CC[CT]
PstI 425 CTGCAG CTGCA^G
RsaI 633 GTAC GT^AC
SacII 22 CCGCGG CCGC^GG
Sau3AI 670 GATC ^GATC
ScaI 632 AGTACT AGT^ACT
SfiI 550 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 460 ACTAGT A^CTAGT
TaqI 672 TCGA T^CGA
Tru9I 493 TTAA T^TAA
TthHB8I 672 TCGA T^CGA
XmnI 821 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 206 GAATTT [GA]^AATT[CT]
332 AAATTT
ApoI 206 GAATTT [GA]^AATT[CT]
332 AAATTT
AvaII 165 GGACC G^G[AT]CC
216 GGACC
BanI 6 GGTGCC G^G[CT][GA]CC
18 GGCACC
BbvI 70 GCTGC GCAGC(8/12)
376 GCTGC
BcnI 150 CCCGG CC^[GC]GG
174 CCGGG
BfaI 461 CTAG C^TAG
818 CTAG
BsmFI 164 GGGAC GGGAC(10/14)
133 GTCCC
BsrFI 15 ACCGGC [GA]^CCGG[CT]
344 ACCGGT
BstNI 324 CCAGG CC^[AT]GG
561 CCTGG
Cfr10I 15 ACCGGC [GA]^CCGG[CT]
344 ACCGGT
Cfr13I 165 GGACC G^G[ACGT]CC
216 GGACC
EcoRII 324 CCAGG ^CC[AT]GG
561 CCTGG
MaeI 461 CTAG C^TAG
818 CTAG
MaeII 131 ACGT A^CGT
545 ACGT
MboII 700 GAAGA GAAGA(8/7)
766 GAAGA
MvaI 324 CCAGG CC^[AT]GG
561 CCTGG
NciI 150 CCCGG CC^[GC]GG
174 CCGGG
NlaIII 295 CATG CATG^
685 CATG
Sau96I 165 GGACC G^G[ACGT]CC
216 GGACC
SmlI 103 CTTGAG C^T[CT][GA]AG
492 CTTAAG
StuI 549 AGGCCT AGG^CCT
609 AGGCCT
TfiI 91 GATTC G^A[AT]TC
154 GAATC
TseI 70 GCTGC G^C[AT]GC
376 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BmyI BsiYI BslI
Bsp1286I BssKI Cac8I DdeI Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeIII MnlI MspI MwoI NlaIV
ScrFI SfaNI SfcI Tsp45I Tsp509I
TspRI