Restriction map for
pF1KE0485
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 469 GAATTT [GA]^AATT[CT]
AflIII 354 ACACGT A^C[GA][CT]GT
AluI 215 AGCT AG^CT
ApoI 469 GAATTT [GA]^AATT[CT]
AspHI 541 GAGCAC G[AT]GC[AT]^C
BamHI 39 GGATCC G^GATCC
BanI 127 GGCACC G^G[CT][GA]CC
BbrPI 355 CACGTG CAC^GTG
BfaI 535 CTAG C^TAG
BglII 263 AGATCT A^GATCT
BsaAI 355 CACGTG [CT]AC^GT[GA]
BsiEI 201 CGGCCG CG[GA][CT]^CG
BsiHKAI 541 GAGCAC G[AT]GC[AT]^C
BspHI 455 TCATGA T^CATGA
BstAPI 420 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 439 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 201 CGGCCG [CT]^GGCC[GA]
EagI 201 CGGCCG C^GGCCG
EclXI 201 CGGCCG C^GGCCG
Eco52I 201 CGGCCG C^GGCCG
EcoNI 402 CCTATATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 439 GGGGCCC [GA]G^G[ACGT]CC[CT]
HaeII 113 AGCGCC [GA]GCGC^[CT]
MaeI 535 CTAG C^TAG
MaeII 356 ACGT A^CGT
MaeIII 274 GTCAC ^GT[ACGT]AC
PmaCI 355 CACGTG CAC^GTG
PmlI 355 CACGTG CAC^GTG
RcaI 455 TCATGA T^CATGA
SexAI 230 ACCTGGT A^CC[AT]GGT
SfcI 267 CTACAG C^T[GA][CT]AG
Tsp45I 274 GTCAC ^GT[GC]AC
Tsp509I 470 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 40 GATCC GGATC(4/5)
39 GGATC
ApaI 22 GGGCCC GGGCC^C
440 GGGCCC
BpmI 580 CTCCAG CTGGAG(16/14)
291 CTGGAG
BsmAI 360 GAGAC GTCTC(1/5)
448 GTCTC
BsmFI 485 GGGAC GGGAC(10/14)
512 GTCCC
BstYI 39 GGATCC [GA]^GATC[CT]
263 AGATCT
CfoI 114 GCGC GCG^C
219 GCGC
DdeI 176 CTAAG C^T[ACGT]AG
556 CTGAG
DpnII 40 GATC ^GATC
264 GATC
HhaI 114 GCGC GCG^C
219 GCGC
HinP1I 114 GCGC G^CGC
219 GCGC
HinfI 180 GAATC G^A[ACGT]TC
585 GAATC
MboI 40 GATC ^GATC
264 GATC
MboII 183 TCTTC GAAGA(8/7)
371 TCTTC
MflI 39 GGATCC [GA]^GATC[CT]
263 AGATCT
MseI 65 TTAA T^TAA
473 TTAA
MslI 351 CACACACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
489 CACCTTTGTG
NdeII 40 GATC ^GATC
264 GATC
NlaIII 208 CATG CATG^
456 CATG
Sau3AI 40 GATC ^GATC
264 GATC
TfiI 180 GAATC G^A[AT]TC
585 GAATC
Tru9I 65 TTAA T^TAA
473 TTAA
TspRI 416 CACTG CA[GC]TG[ACGT][ACGT]^
478 CACTG
XhoII 39 GGATCC [GA]^GATC[CT]
263 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AvaII BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII ItaI MnlI MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI SfaNI TseI XcmI