Restriction map for
pF1KE0350
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AvrII BamHI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BsaBI BsaI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfuI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
TaqI TthHB8I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1938 GTCTAC GT^[AC][GT]AC
AccIII 2144 TCCGGA T^CCGGA
AseI 1192 ATTAAT AT^TAAT
AsnI 1192 ATTAAT AT^TAAT
Asp700I 1440 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 186 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 50 TGCGCA TGC^GCA
BalI 1557 TGGCCA TGG^CCA
BanI 785 GGCGCC G^G[CT][GA]CC
BbeI 785 GGCGCC GGCGC^C
BbvCI 2218 GCTGAGG CCTCAGC(-5/-2)
BfaI 1879 CTAG C^TAG
BglII 134 AGATCT A^GATCT
BsaAI 437 TACGTG [CT]AC^GT[GA]
BsaWI 2144 TCCGGA [AT]^CCGG[AT]
BseAI 2144 TCCGGA T^CCGGA
BsmAI 184 GAGAC GTCTC(1/5)
BsmI 1753 GCATTC GAATGC(1/-1)
BspEI 2144 TCCGGA T^CCGGA
BsrDI 46 GCAATG GCAATG(2/0)
BsrFI 782 ACCGGC [GA]^CCGG[CT]
BstAPI 1340 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 782 ACCGGC [GA]^CCGG[CT]
DraI 1791 TTTAAA TTT^AAA
DraIII 260 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 982 GACAGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 25 GAAGAG CTCTTC(1/4)
FauI 1271 CCCGC CCCGC(4/6)
FspI 50 TGCGCA TGC^GCA
HaeII 785 GGCGCC [GA]GCGC^[CT]
HgaI 1094 GACGC GACGC(5/10)
KasI 785 GGCGCC G^GCGCC
Ksp632I 25 GAAGAG CTCTTC(1/4)
MaeI 1879 CTAG C^TAG
MluNI 1557 TGGCCA TGG^CCA
MroI 2144 TCCGGA T^CCGGA
MscI 1557 TGGCCA TGG^CCA
MspA1I 20 CAGCTG C[AC]G^C[GT]G
NarI 785 GGCGCC GG^CGCC
PflFI 186 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1417 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1192 ATTAAT AT^TAAT
PstI 888 CTGCAG CTGCA^G
PvuII 20 CAGCTG CAG^CTG
ScaI 1814 AGTACT AGT^ACT
SfoI 785 GGCGCC GGC^GCC
SgrAI 781 CACCGGCG C[GA]^CCGG[CT]G
SphI 1260 GCATGC GCATG^C
Tth111I 186 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1417 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1878 TCTAGA T^CTAGA
XmnI 1440 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 785 GGCGCC G[GA]^CG[CT]C
1094 GACGCC
AfaI 1254 GTAC GT^AC
1815 GTAC
AflIII 1562 ACATGT A^C[GA][CT]GT
1640 ACATGT
AlwI 1368 GATCC GGATC(4/5)
1382 GGATC
AspHI 1660 GTGCTC G[AT]GC[AT]^C
2022 GAGCAC
BlpI 1264 GCTGAGC GC^T[ACGT]AGC
2019 GCTGAGC
Bpu1102I 1264 GCTGAGC GC^T[ACGT]AGC
2019 GCTGAGC
BsaHI 785 GGCGCC G[GA]^CG[CT]C
1094 GACGCC
BsgI 2003 CTGCAC GTGCAG(16/14)
352 GTGCAG
BsiHKAI 1660 GTGCTC G[AT]GC[AT]^C
2022 GAGCAC
BspLU11I 1562 ACATGT A^CATGT
1640 ACATGT
BstYI 134 AGATCT [GA]^GATC[CT]
1382 GGATCT
CelII 1264 GCTGAGC GC^T[ACGT]AGC
2019 GCTGAGC
CfoI 51 GCGC GCG^C
786 GCGC
EaeI 1287 TGGCCG [CT]^GGCC[GA]
1557 TGGCCA
EcoNI 1239 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1671 CCTGAGAAAGG
EcoRV 1771 GATATC GAT^ATC
2140 GATATC
EcoT14I 228 CCAAGG C^C[AT][AT]GG
1970 CCAAGG
EcoT22I 432 ATGCAT ATGCA^T
1048 ATGCAT
HapII 783 CCGG C^CGG
2145 CCGG
HhaI 51 GCGC GCG^C
786 GCGC
Hin1I 785 GGCGCC G[GA]^CG[CT]C
1094 GACGCC
HinP1I 51 GCGC G^CGC
786 GCGC
HpaII 783 CCGG C^CGG
2145 CCGG
MflI 134 AGATCT [GA]^GATC[CT]
1382 GGATCT
MspI 783 CCGG C^CGG
2145 CCGG
NsiI 432 ATGCAT ATGCA^T
1048 ATGCAT
RsaI 1254 GTAC GT^AC
1815 GTAC
SexAI 1238 ACCTGGT A^CC[AT]GGT
2191 ACCTGGT
SspI 1202 AATATT AAT^ATT
1441 AATATT
StyI 228 CCAAGG C^C[AT][AT]GG
1970 CCAAGG
Tsp45I 365 GTGAC ^GT[GC]AC
1851 GTCAC
XhoII 134 AGATCT [GA]^GATC[CT]
1382 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BanII BbsI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
RcaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp509I TspRI