Restriction map for
pF1KB4308
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeII MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI NarI NcoI
NdeI NdeII NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 6 GACGCC G[GA]^CG[CT]C
AfaI 64 GTAC GT^AC
ApaI 28 GGGCCC GGGCC^C
AspHI 55 GTGCTC G[AT]GC[AT]^C
BanI 289 GGTGCC G^G[CT][GA]CC
BanII 28 GGGCCC G[GA]GC[CT]^C
BcnI 477 CCGGG CC^[GC]GG
BfaI 496 CTAG C^TAG
BglI 463 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 76 CTGGAG CTGGAG(16/14)
BsaHI 6 GACGCC G[GA]^CG[CT]C
BsaI 125 GAGACC GGTCTC(1/5)
BsiEI 185 CGGCCG CG[GA][CT]^CG
BsiHKAI 55 GTGCTC G[AT]GC[AT]^C
BsmAI 125 GAGAC GTCTC(1/5)
BsrDI 218 GCAATG GCAATG(2/0)
BsrFI 187 GCCGGC [GA]^CCGG[CT]
BstXI 368 CCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 187 GCCGGC [GA]^CCGG[CT]
DsaI 282 CCACGG C^C[GA][CT]GG
EaeI 185 CGGCCG [CT]^GGCC[GA]
EagI 185 CGGCCG C^GGCCG
EclXI 185 CGGCCG C^GGCCG
Eco52I 185 CGGCCG C^GGCCG
Hin1I 6 GACGCC G[GA]^CG[CT]C
MaeI 496 CTAG C^TAG
MseI 148 TTAA T^TAA
MslI 283 CACGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 187 GCCGGC GCC^GGC
NciI 477 CCGGG CC^[GC]GG
NgoMIV 187 GCCGGC G^CCGGC
NheI 495 GCTAGC G^CTAGC
NspI 499 GCATGT [GA]CATG^[CT]
PleI 181 GACTC GAGTC(4/5)
PshAI 166 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 64 GTAC GT^AC
TaqI 92 TCGA T^CGA
TfiI 101 GAATC G^A[AT]TC
Tru9I 148 TTAA T^TAA
TthHB8I 92 TCGA T^CGA
XcmI 362 CCAACCCCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 261 AGCT AG^CT
313 AGCT
BseRI 442 CTCCTC GAGGAG(10/8)
79 GAGGAG
BsgI 202 CTGCAC GTGCAG(16/14)
301 CTGCAC
BsiYI 362 CCAACCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
366 CCCCAGGGTGG
BslI 362 CCAACCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
366 CCCCAGGGTGG
BsrI 240 CCAGT ACTGG(1/-1)
395 CCAGT
BstNI 129 CCTGG CC^[AT]GG
368 CCAGG
DdeI 263 CTAAG C^T[ACGT]AG
413 CTGAG
EcoRII 129 CCTGG ^CC[AT]GG
368 CCAGG
FauI 320 GCGGG CCCGC(4/6)
531 GCGGG
HapII 188 CCGG C^CGG
477 CCGG
HgaI 6 GACGC GACGC(5/10)
72 GACGC
HinfI 101 GAATC G^A[ACGT]TC
181 GACTC
HpaII 188 CCGG C^CGG
477 CCGG
MspI 188 CCGG C^CGG
477 CCGG
MvaI 129 CCTGG CC^[AT]GG
368 CCAGG
PpuMI 164 AGGACCC [GA]G^G[AT]CC[CT]
533 GGGACCT
SfaNI 271 GATGC GCATC(5/9)
42 GCATC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AlwNI AvaII BbvI
BmyI BsaJI BsmFI Bsp1286I BspMI
BssKI BstF5I BstUI Cac8I CfoI
Cfr13I DraII EcoO109I Fnu4HI FokI
HaeIII HhaI HinP1I ItaI MaeIII
MnlI MvnI MwoI NlaIII NlaIV
Sau96I ScrFI TseI Tsp45I TspRI