Restriction map for
pF1KB7787
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AcyI AflII AgeI AlwNI AocI
Aor51HI AscI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 161 GGTACC G^GTACC
AccI 1300 GTCTAC GT^[AC][GT]AC
AflIII 394 ACACGT A^C[GA][CT]GT
AhdI 292 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 61 GTGCAC G^TGCAC
AlwI 238 GATCC GGATC(4/5)
ApaI 107 GGGCCC GGGCC^C
ApaLI 61 GTGCAC G^TGCAC
AseI 306 ATTAAT AT^TAAT
AsnI 306 ATTAAT AT^TAAT
Asp718I 161 GGTACC G^GTACC
AspEI 292 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 254 GGACC G^G[AT]CC
BanII 107 GGGCCC G[GA]GC[CT]^C
BbsI 1146 GTCTTC GAAGAC(2/6)
BbvI 4 GCAGC GCAGC(8/12)
BclI 695 TGATCA T^GATCA
BglI 100 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1146 GTCTTC GAAGAC(2/6)
BsaAI 395 CACGTA [CT]AC^GT[GA]
BsgI 179 CTGCAC GTGCAG(16/14)
Bsp1407I 739 TGTACA T^GTACA
BspMI 177 ACCTGC ACCTGC(4/8)
BsrGI 739 TGTACA T^GTACA
Cac8I 669 GCGAGC GC[ACGT]^[ACGT]GC
Eam1105I 292 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 360 GAAGAG CTCTTC(1/4)
EcoNI 464 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 441 CCAAGG C^C[AT][AT]GG
FbaI 695 TGATCA T^GATCA
Fnu4HI 4 GCAGC GC^[ACGT]GC
ItaI 4 GCAGC GC^[ACGT]GC
KpnI 161 GGTACC GGTAC^C
Ksp632I 360 GAAGAG CTCTTC(1/4)
MspA1I 355 CAGCTG C[AC]G^C[GT]G
NdeI 483 CATATG CA^TATG
NspI 1296 GCATGT [GA]CATG^[CT]
PflMI 30 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1327 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 306 ATTAAT AT^TAAT
PvuII 355 CAGCTG CAG^CTG
SmlI 1291 CTTGAG C^T[CT][GA]AG
SspBI 739 TGTACA T^GTACA
StuI 1259 AGGCCT AGG^CCT
StyI 441 CCAAGG C^C[AT][AT]GG
TaqI 47 TCGA T^CGA
TseI 4 GCAGC G^C[AT]GC
TthHB8I 47 TCGA T^CGA
Van91I 30 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 162 GTAC GT^AC
740 GTAC
AspHI 61 GTGCAC G[AT]GC[AT]^C
1100 GTGCTC
BsaI 280 GAGACC GGTCTC(1/5)
642 GAGACC
BsaJI 312 CCAGGG C^C[ACGT][ACGT]GG
441 CCAAGG
BsiHKAI 61 GTGCAC G[AT]GC[AT]^C
1100 GTGCTC
BsmAI 280 GAGAC GTCTC(1/5)
642 GAGAC
BstEII 39 GGTGACC G^GT[ACGT]ACC
1285 GGTCACC
BstPI 39 GGTGACC G^GT[ACGT]ACC
1285 GGTCACC
EcoO65I 39 GGTGACC G^GT[ACGT]ACC
1285 GGTCACC
EcoRI 1046 GAATTC G^AATTC
1130 GAATTC
HindIII 839 AAGCTT A^AGCTT
924 AAGCTT
MaeII 263 ACGT A^CGT
396 ACGT
MwoI 100 GCCCAGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1093 GCCTTCAGTGC
PleI 298 GAGTC GAGTC(4/5)
1144 GAGTC
RsaI 162 GTAC GT^AC
740 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BanI
BfaI BmyI BpmI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FokI HaeIII
HinfI HphI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I Tsp45I Tsp509I
TspRI XmnI