Restriction map for
pF1KB7750
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BanI BbeI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 773 CGCG CG^CG
AccIII 1085 TCCGGA T^CCGGA
AlwNI 262 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 436 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 972 GAGCAC G[AT]GC[AT]^C
AspI 62 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1270 CCCGAG C^[CT]CG[GA]G
BalI 374 TGGCCA TGG^CCA
BamHI 1328 GGATCC G^GATCC
BbrPI 637 CACGTG CAC^GTG
BbsI 109 GAAGAC GAAGAC(2/6)
BbvCI 1013 CCTCAGC CCTCAGC(-5/-2)
BglI 1052 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 109 GAAGAC GAAGAC(2/6)
BsaAI 637 CACGTG [CT]AC^GT[GA]
BsaI 920 GAGACC GGTCTC(1/5)
BseAI 1085 TCCGGA T^CCGGA
BsiEI 808 CGGCCG CG[GA][CT]^CG
BsiHKAI 972 GAGCAC G[AT]GC[AT]^C
BsmBI 1251 GAGACG CGTCTC(1/5)
BsoBI 1270 CCCGAG C^[CT]CG[GA]G
BspEI 1085 TCCGGA T^CCGGA
BstUI 773 CGCG CG^CG
BstXI 691 CCACCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 161 CCATGG C^C[GA][CT]GG
EagI 808 CGGCCG C^GGCCG
EclXI 808 CGGCCG C^GGCCG
Eco52I 808 CGGCCG C^GGCCG
EcoRV 415 GATATC GAT^ATC
HgaI 1253 GACGC GACGC(5/10)
MaeII 638 ACGT A^CGT
MluNI 374 TGGCCA TGG^CCA
MroI 1085 TCCGGA T^CCGGA
MscI 374 TGGCCA TGG^CCA
MseI 515 TTAA T^TAA
MvnI 773 CGCG CG^CG
NcoI 161 CCATGG C^CATGG
NotI 807 GCGGCCGC GC^GGCCGC
NspI 305 ACATGC [GA]CATG^[CT]
PflFI 62 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 637 CACGTG CAC^GTG
PmlI 637 CACGTG CAC^GTG
SfcI 942 CTACAG C^T[GA][CT]AG
TfiI 1153 GATTC G^A[AT]TC
Tru9I 515 TTAA T^TAA
Tsp45I 835 GTGAC ^GT[GC]AC
Tth111I 62 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1148 CCAAGGATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 436 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 710 GTAC GT^AC
1173 GTAC
AflIII 636 ACACGT A^C[GA][CT]GT
638 ACGTGT
AocI 240 CCTCAGG CC^T[ACGT]AGG
664 CCTCAGG
BcnI 703 CCGGG CC^[GC]GG
968 CCCGG
BpmI 54 CTCCAG CTGGAG(16/14)
749 CTCCAG
BsaWI 1085 TCCGGA [AT]^CCGG[AT]
1331 TCCGGT
BsgI 196 CTGCAC GTGCAG(16/14)
1315 CTGCAC
BssSI 199 CACGAG CACGAG(-5/-1)
1093 CACGAG
BstYI 428 GGATCT [GA]^GATC[CT]
1328 GGATCC
Bsu36I 240 CCTCAGG CC^T[ACGT]AGG
664 CCTCAGG
Eco81I 240 CCTCAGG CC^T[ACGT]AGG
664 CCTCAGG
FauI 774 GCGGG CCCGC(4/6)
1169 GCGGG
HaeII 398 AGCGCC [GA]GCGC^[CT]
1193 AGCGCC
MaeIII 74 GTTAC ^GT[ACGT]AC
835 GTGAC
MflI 428 GGATCT [GA]^GATC[CT]
1328 GGATCC
MslI 366 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
828 CATTGGGGTG
NciI 703 CCGGG CC^[GC]GG
968 CCCGG
PflMI 261 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
892 CCATCTTGTGG
PvuII 357 CAGCTG CAG^CTG
864 CAGCTG
RsaI 710 GTAC GT^AC
1173 GTAC
SapI 172 GAAGAGC GCTCTTC(1/4)
219 GAAGAGC
TaqI 122 TCGA T^CGA
380 TCGA
TthHB8I 122 TCGA T^CGA
380 TCGA
Van91I 261 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
892 CCATCTTGTGG
XhoII 428 GGATCT [GA]^GATC[CT]
1328 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp509I TspRI