Restriction map for
pF1KB7729
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MluI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 570 TCCGGA T^CCGGA
AhdI 142 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 142 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 368 TGGCCA TGG^CCA
BanII 1186 GAGCTC G[GA]GC[CT]^C
BbrPI 1012 CACGTG CAC^GTG
BpmI 23 CTCCAG CTGGAG(16/14)
BsaAI 1012 CACGTG [CT]AC^GT[GA]
BsaWI 570 TCCGGA [AT]^CCGG[AT]
BseAI 570 TCCGGA T^CCGGA
BsgI 452 CTGCAC GTGCAG(16/14)
BsmAI 1088 GAGAC GTCTC(1/5)
BsmFI 492 GTCCC GGGAC(10/14)
BspEI 570 TCCGGA T^CCGGA
BspHI 186 TCATGA T^CATGA
BstYI 1204 GGATCT [GA]^GATC[CT]
DraI 738 TTTAAA TTT^AAA
DsaI 528 CCATGG C^C[GA][CT]GG
EaeI 368 TGGCCA [CT]^GGCC[GA]
Eam1105I 142 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 782 CTCTTC CTCTTC(1/4)
EcoT14I 528 CCATGG C^C[AT][AT]GG
HapII 571 CCGG C^CGG
HindIII 93 AAGCTT A^AGCTT
HpaII 571 CCGG C^CGG
HphI 649 TCACC GGTGA(8/7)
Ksp632I 782 CTCTTC CTCTTC(1/4)
MfeI 239 CAATTG C^AATTG
MflI 1204 GGATCT [GA]^GATC[CT]
MluNI 368 TGGCCA TGG^CCA
MroI 570 TCCGGA T^CCGGA
MscI 368 TGGCCA TGG^CCA
MspA1I 1199 CAGCTG C[AC]G^C[GT]G
MspI 571 CCGG C^CGG
MunI 239 CAATTG C^AATTG
NcoI 528 CCATGG C^CATGG
PmaCI 1012 CACGTG CAC^GTG
PmlI 1012 CACGTG CAC^GTG
PvuII 1199 CAGCTG CAG^CTG
RcaI 186 TCATGA T^CATGA
SacI 1186 GAGCTC GAGCT^C
ScaI 1255 AGTACT AGT^ACT
SexAI 683 ACCAGGT A^CC[AT]GGT
SfcI 749 CTATAG C^T[GA][CT]AG
StyI 528 CCATGG C^C[AT][AT]GG
TaqI 480 TCGA T^CGA
Tsp45I 1010 GTCAC ^GT[GC]AC
TthHB8I 480 TCGA T^CGA
XhoII 1204 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 1165 ACATGT A^C[GA][CT]GT
1234 ACATGT
AlwNI 47 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
595 CAGGCACTG
Asp700I 545 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1033 GAAGGATTTC
AspHI 376 GAGCAC G[AT]GC[AT]^C
1186 GAGCTC
BfaI 136 CTAG C^TAG
953 CTAG
BmyI 376 GAGCAC G[AGT]GC[ACT]^C
1186 GAGCTC
BseRI 813 GAGGAG GAGGAG(10/8)
1183 GAGGAG
BsiHKAI 376 GAGCAC G[AT]GC[AT]^C
1186 GAGCTC
Bsp1286I 376 GAGCAC G[AGT]GC[ACT]^C
1186 GAGCTC
BspLU11I 1165 ACATGT A^CATGT
1234 ACATGT
Cac8I 95 GCTTGC GC[ACGT]^[ACGT]GC
295 GCTTGC
EcoRI 471 GAATTC G^AATTC
1226 GAATTC
EcoT22I 827 ATGCAT ATGCA^T
1100 ATGCAT
MaeI 136 CTAG C^TAG
953 CTAG
MaeII 1013 ACGT A^CGT
1061 ACGT
MaeIII 818 GTTAC ^GT[ACGT]AC
1010 GTCAC
MwoI 106 GCTGTTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
718 GCTGCTGCTGC
NsiI 827 ATGCAT ATGCA^T
1100 ATGCAT
NspI 1165 ACATGT [GA]CATG^[CT]
1234 ACATGT
PflMI 7 CCAGTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
280 CCATCCTTTGG
PleI 21 GACTC GAGTC(4/5)
148 GAGTC
Van91I 7 CCAGTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
280 CCATCCTTTGG
XmnI 545 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1033 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbsI BbvI BpuAI BsaJI
BsiYI BslI BsrDI BsrI BssKI
BstF5I BstNI BstXI Cfr13I DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI