Restriction map for
pF1KB7617
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BglI BglII
BlnI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsiEI
BsiWI BsiYI BslI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cac8I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI ItaI
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI MwoI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TseI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 577 CGCG CG^CG
AfaI 376 GTAC GT^AC
AflII 34 CTTAAG C^TTAAG
AhdI 589 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 129 GATCC GGATC(4/5)
Asp700I 787 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 589 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 276 GAGCTC G[AT]GC[AT]^C
AvaII 573 GGACC G^G[AT]CC
BanII 276 GAGCTC G[GA]GC[CT]^C
BcnI 599 CCGGG CC^[GC]GG
BfrI 34 CTTAAG C^TTAAG
BlpI 534 GCTGAGC GC^T[ACGT]AGC
BmyI 276 GAGCTC G[AGT]GC[ACT]^C
BpmI 397 CTGGAG CTGGAG(16/14)
Bpu1102I 534 GCTGAGC GC^T[ACGT]AGC
BsaBI 388 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 605 GTGCAG GTGCAG(16/14)
BsiHKAI 276 GAGCTC G[AT]GC[AT]^C
BsmAI 521 GAGAC GTCTC(1/5)
Bsp1286I 276 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 375 TGTACA T^GTACA
BspMI 685 ACCTGC ACCTGC(4/8)
BsrDI 500 CATTGC GCAATG(2/0)
BsrGI 375 TGTACA T^GTACA
BstF5I 801 GGATG GGATG(2/0)
BstNI 444 CCAGG CC^[AT]GG
BstUI 577 CGCG CG^CG
BstYI 128 AGATCC [GA]^GATC[CT]
CelII 534 GCTGAGC GC^T[ACGT]AGC
Cfr13I 573 GGACC G^G[ACGT]CC
EaeI 407 CGGCCA [CT]^GGCC[GA]
Eam1105I 589 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 169 GAATTC G^AATTC
EcoRII 444 CCAGG ^CC[AT]GG
FokI 801 GGATG GGATG(9/13)
HaeIII 408 GGCC GG^CC
HapII 599 CCGG C^CGG
HincII 893 GTCAAC GT[CT]^[GA]AC
HindII 893 GTCAAC GT[CT]^[GA]AC
HindIII 136 AAGCTT A^AGCTT
HpaII 599 CCGG C^CGG
HphI 320 TCACC GGTGA(8/7)
MaeII 616 ACGT A^CGT
MamI 388 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 128 AGATCC [GA]^GATC[CT]
MspI 599 CCGG C^CGG
MvaI 444 CCAGG CC^[AT]GG
MvnI 577 CGCG CG^CG
NciI 599 CCGGG CC^[GC]GG
PacI 823 TTAATTAA TTAAT^TAA
PleI 434 GAGTC GAGTC(4/5)
RsaI 376 GTAC GT^AC
SacI 276 GAGCTC GAGCT^C
Sau96I 573 GGACC G^G[ACGT]CC
SmlI 34 CTTAAG C^T[CT][GA]AG
SspBI 375 TGTACA T^GTACA
SspI 109 AATATT AAT^ATT
TaqI 280 TCGA T^CGA
TfiI 440 GATTC G^A[AT]TC
TspRI 767 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 280 TCGA T^CGA
XhoII 128 AGATCC [GA]^GATC[CT]
XmnI 787 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 576 CCGC CCGC(-3/-1)
563 GCGG
BfaI 529 CTAG C^TAG
889 CTAG
BsmFI 81 GGGAC GGGAC(10/14)
549 GTCCC
BsmI 42 GCATTC GAATGC(1/-1)
791 GCATTC
BsrI 243 CCAGT ACTGG(1/-1)
415 CCAGT
BssKI 444 CCAGG ^CC[ACGT]GG
599 CCGGG
DpnII 129 GATC ^GATC
511 GATC
HinfI 434 GAGTC G^A[ACGT]TC
440 GATTC
MaeI 529 CTAG C^TAG
889 CTAG
MboI 129 GATC ^GATC
511 GATC
MslI 615 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
792 CATTCAAATG
NdeII 129 GATC ^GATC
511 GATC
NlaIII 360 CATG CATG^
373 CATG
Sau3AI 129 GATC ^GATC
511 GATC
ScrFI 444 CCAGG CC^[ACGT]GG
599 CCGGG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AcsI AluI ApoI DdeI MaeIII
MboII MnlI MseI Tru9I Tsp509I