Restriction map for
pF1KE0678
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BalI BamHI BanII BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 71 AAATTT [GA]^AATT[CT]
AhdI 613 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 478 GTGCAC G^TGCAC
AlwI 922 GATCC GGATC(4/5)
AlwNI 226 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 478 GTGCAC G^TGCAC
ApoI 71 AAATTT [GA]^AATT[CT]
AspEI 613 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1057 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 475 CACGTG CAC^GTG
BfaI 1132 CTAG C^TAG
BsaAI 475 CACGTG [CT]AC^GT[GA]
BsmBI 715 GAGACG CGTCTC(1/5)
DraIII 1157 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 536 CCATGG C^C[GA][CT]GG
Eam1105I 613 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 898 CCTAATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 657 CCGG C^CGG
HgaI 984 GCGTC GACGC(5/10)
HpaII 657 CCGG C^CGG
MaeI 1132 CTAG C^TAG
MspI 657 CCGG C^CGG
NcoI 536 CCATGG C^CATGG
NdeI 638 CATATG CA^TATG
PflFI 1057 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 475 CACGTG CAC^GTG
PmlI 475 CACGTG CAC^GTG
PstI 409 CTGCAG CTGCA^G
SapI 372 GCTCTTC GCTCTTC(1/4)
ScaI 682 AGTACT AGT^ACT
SfcI 409 CTGCAG C^T[GA][CT]AG
SmlI 937 CTTGAG C^T[CT][GA]AG
SspI 451 AATATT AAT^ATT
TaqI 782 TCGA T^CGA
TfiI 1021 GATTC G^A[AT]TC
Tth111I 1057 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 782 TCGA T^CGA
XcmI 548 CCAAGCCTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Asp700I 369 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1068 GAAGGAGTTC
AvaII 300 GGACC G^G[AT]CC
620 GGTCC
BanI 544 GGCACC G^G[CT][GA]CC
561 GGCACC
BbvI 502 GCTGC GCAGC(8/12)
510 GCTGC
BsaI 317 GAGACC GGTCTC(1/5)
660 GAGACC
BsmFI 1106 GGGAC GGGAC(10/14)
621 GTCCC
Bsp1407I 117 TGTACA T^GTACA
702 TGTACA
BspLU11I 284 ACATGT A^CATGT
1033 ACATGT
BsrGI 117 TGTACA T^GTACA
702 TGTACA
BstF5I 858 GGATG GGATG(2/0)
1167 GGATG
CfoI 183 GCGC GCG^C
420 GCGC
DpnII 219 GATC ^GATC
922 GATC
EarI 373 CTCTTC CTCTTC(1/4)
873 GAAGAG
EcoT14I 536 CCATGG C^C[AT][AT]GG
1136 CCTTGG
Fnu4HI 502 GCTGC GC^[ACGT]GC
510 GCTGC
FokI 858 GGATG GGATG(9/13)
1167 GGATG
HaeIII 396 GGCC GG^CC
655 GGCC
HhaI 183 GCGC GCG^C
420 GCGC
HinP1I 183 GCGC G^CGC
420 GCGC
HincII 52 GTCAAC GT[CT]^[GA]AC
610 GTTGAC
HindII 52 GTCAAC GT[CT]^[GA]AC
610 GTTGAC
HphI 1162 GGTGA GGTGA(8/7)
330 TCACC
ItaI 502 GCTGC GC^[ACGT]GC
510 GCTGC
Ksp632I 373 CTCTTC CTCTTC(1/4)
873 GAAGAG
MboI 219 GATC ^GATC
922 GATC
MslI 285 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
291 CATGGTGGTG
MwoI 176 GCGTGGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
411 GCAGAACCAGC
NdeII 219 GATC ^GATC
922 GATC
NspI 284 ACATGT [GA]CATG^[CT]
1033 ACATGT
PleI 1057 GACTC GAGTC(4/5)
955 GAGTC
Sau3AI 219 GATC ^GATC
922 GATC
SspBI 117 TGTACA T^GTACA
702 TGTACA
StyI 536 CCATGG C^C[AT][AT]GG
1136 CCTTGG
TseI 502 GCTGC G^C[AT]GC
510 GCTGC
TspRI 29 CAGTG CA[GC]TG[ACGT][ACGT]^
365 CAGTG
XmnI 369 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1068 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AflIII AluI AspHI BbsI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrDI BsrI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII HinfI MaeII
MaeIII MboII MnlI MseI MvaI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI Tru9I Tsp45I Tsp509I