Restriction map for
pF1KB8475
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AccII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BglI
BlnI BsaAI BsaI BsgI BsiEI
BsiWI BsmBI BsmFI BspDI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NlaIV NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PstI
PvuI RcaI RsrII SacII SbfI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 1046 GACGTC GACGT^C
AccI 2270 GTCGAC GT^[AC][GT]AC
AccIII 270 TCCGGA T^CCGGA
AcyI 1046 GACGTC G[GA]^CG[CT]C
AlwNI 2300 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1819 ATTAAT AT^TAAT
AsnI 1819 ATTAAT AT^TAAT
Asp700I 2501 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 941 CCCGAG C^[CT]CG[GA]G
BalI 2061 TGGCCA TGG^CCA
BbvI 676 GCAGC GCAGC(8/12)
BciVI 2564 GTATCC GTATCC(6/5)
BsaBI 1753 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1046 GACGTC G[GA]^CG[CT]C
BsaWI 270 TCCGGA [AT]^CCGG[AT]
BseAI 270 TCCGGA T^CCGGA
BsoBI 941 CCCGAG C^[CT]CG[GA]G
BspEI 270 TCCGGA T^CCGGA
BspLU11I 2274 ACATGT A^CATGT
BspMI 2560 GCAGGT ACCTGC(4/8)
BsrDI 2513 GCAATG GCAATG(2/0)
BssKI 741 CCTGG ^CC[ACGT]GG
BstEII 1703 GGTTACC G^GT[ACGT]ACC
BstNI 741 CCTGG CC^[AT]GG
BstPI 1703 GGTTACC G^GT[ACGT]ACC
Cac8I 2196 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1116 GCGC GCG^C
DrdI 1086 GACAGACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2481 CCACGG C^C[GA][CT]GG
EaeI 2061 TGGCCA [CT]^GGCC[GA]
EcoNI 327 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1703 GGTTACC G^GT[ACGT]ACC
EcoRII 741 CCTGG ^CC[AT]GG
EcoT14I 89 CCAAGG C^C[AT][AT]GG
FauI 1929 GCGGG CCCGC(4/6)
Fnu4HI 676 GCAGC GC^[ACGT]GC
HaeII 1115 AGCGCC [GA]GCGC^[CT]
HapII 271 CCGG C^CGG
HgaI 1812 GACGC GACGC(5/10)
HhaI 1116 GCGC GCG^C
Hin1I 1046 GACGTC G[GA]^CG[CT]C
HinP1I 1116 GCGC G^CGC
HincII 2270 GTCGAC GT[CT]^[GA]AC
HindII 2270 GTCGAC GT[CT]^[GA]AC
HpaII 271 CCGG C^CGG
ItaI 676 GCAGC GC^[ACGT]GC
MamI 1753 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2061 TGGCCA TGG^CCA
MroI 270 TCCGGA T^CCGGA
MscI 2061 TGGCCA TGG^CCA
MspI 271 CCGG C^CGG
MvaI 741 CCTGG CC^[AT]GG
NdeI 119 CATATG CA^TATG
PshAI 495 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1819 ATTAAT AT^TAAT
SalI 2270 GTCGAC G^TCGAC
SapI 2455 GCTCTTC GCTCTTC(1/4)
ScaI 444 AGTACT AGT^ACT
ScrFI 741 CCTGG CC^[ACGT]GG
SpeI 480 ACTAGT A^CTAGT
StyI 89 CCAAGG C^C[AT][AT]GG
TseI 676 GCAGC G^C[AT]GC
XmnI 2501 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflII 1735 CTTAAG C^TTAAG
2086 CTTAAG
AflIII 1091 ACGTGT A^C[GA][CT]GT
2274 ACATGT
BbsI 1465 GAAGAC GAAGAC(2/6)
1221 GTCTTC
BfrI 1735 CTTAAG C^TTAAG
2086 CTTAAG
BglII 864 AGATCT A^GATCT
2629 AGATCT
BlpI 1069 GCTCAGC GC^T[ACGT]AGC
1126 GCTGAGC
BpmI 1238 CTCCAG CTGGAG(16/14)
947 CTGGAG
Bpu1102I 1069 GCTCAGC GC^T[ACGT]AGC
1126 GCTGAGC
BpuAI 1465 GAAGAC GAAGAC(2/6)
1221 GTCTTC
BseRI 1393 GAGGAG GAGGAG(10/8)
1970 GAGGAG
BstBI 524 TTCGAA TT^CGAA
2459 TTCGAA
CelII 1069 GCTCAGC GC^T[ACGT]AGC
1126 GCTGAGC
EcoRV 1159 GATATC GAT^ATC
1426 GATATC
HaeIII 2062 GGCC GG^CC
2183 GGCC
HindIII 641 AAGCTT A^AGCTT
1917 AAGCTT
NspI 2274 ACATGT [GA]CATG^[CT]
2523 GCATGT
NspV 524 TTCGAA TT^CGAA
2459 TTCGAA
PflMI 504 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1651 CCAGAATGTGG
PvuII 1124 CAGCTG CAG^CTG
1748 CAGCTG
SacI 988 GAGCTC GAGCT^C
2453 GAGCTC
SfuI 524 TTCGAA TT^CGAA
2459 TTCGAA
SspI 803 AATATT AAT^ATT
1520 AATATT
Van91I 504 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1651 CCAGAATGTGG
XcmI 504 CCATCACATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2242 CCAAAAGAAAACTGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BsrGI
BsrI BstF5I BstYI Cfr13I DdeI
DpnII DraI EarI FokI HinfI
HphI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MwoI NdeII NlaIII
PleI RsaI Sau3AI Sau96I SfaNI
SmlI SspBI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XhoII