Restriction map for
pF1KB5754
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BanI BbeI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AhdI 857 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1577 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 751 ATTAAT AT^TAAT
AsnI 751 ATTAAT AT^TAAT
AspEI 857 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 37 GAGCAC G[AT]GC[AT]^C
AvaII 520 GGTCC G^G[AT]CC
BalI 1925 TGGCCA TGG^CCA
BamHI 237 GGATCC G^GATCC
BanII 1651 GGGCTC G[GA]GC[CT]^C
BbrPI 551 CACGTG CAC^GTG
BbvI 1567 GCAGC GCAGC(8/12)
BciVI 1671 GTATCC GTATCC(6/5)
BclI 453 TGATCA T^GATCA
BsaBI 238 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 745 GAGGAG GAGGAG(10/8)
BsiHKAI 37 GAGCAC G[AT]GC[AT]^C
BsmFI 307 GGGAC GGGAC(10/14)
BspMI 1664 ACCTGC ACCTGC(4/8)
DraI 1867 TTTAAA TTT^AAA
DraII 97 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 1925 TGGCCA [CT]^GGCC[GA]
Eam1105I 857 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1690 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 97 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 647 GAATTC G^AATTC
EcoRV 877 GATATC GAT^ATC
FbaI 453 TGATCA T^GATCA
Fnu4HI 1567 GCAGC GC^[ACGT]GC
HincII 702 GTCAAC GT[CT]^[GA]AC
HindII 702 GTCAAC GT[CT]^[GA]AC
ItaI 1567 GCAGC GC^[ACGT]GC
MamI 238 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1925 TGGCCA TGG^CCA
MscI 1925 TGGCCA TGG^CCA
MspA1I 1568 CAGCTG C[AC]G^C[GT]G
MwoI 1567 GCAGCTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 110 ACATGC [GA]CATG^[CT]
PmaCI 551 CACGTG CAC^GTG
PmlI 551 CACGTG CAC^GTG
PshAI 1744 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 751 ATTAAT AT^TAAT
PvuII 1568 CAGCTG CAG^CTG
SexAI 691 ACCTGGT A^CC[AT]GGT
SfcI 1571 CTGTAG C^T[GA][CT]AG
SspI 1267 AATATT AAT^ATT
TseI 1567 GCAGC G^C[AT]GC
XbaI 393 TCTAGA T^CTAGA
XcmI 281 CCAATTATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 385 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
462 GAAGACTTTC
BmyI 37 GAGCAC G[AGT]GC[ACT]^C
1651 GGGCTC
BpmI 122 CTCCAG CTGGAG(16/14)
1955 CTCCAG
BsaAI 74 TACGTG [CT]AC^GT[GA]
551 CACGTG
BsaI 217 GAGACC GGTCTC(1/5)
1686 GAGACC
BsgI 548 CTGCAC GTGCAG(16/14)
962 CTGCAC
BsmI 543 GCATTC GAATGC(1/-1)
2065 GCATTC
Bsp1286I 37 GAGCAC G[AGT]GC[ACT]^C
1651 GGGCTC
BsrI 1560 ACTGG ACTGG(1/-1)
42 CCAGT
BssKI 692 CCTGG ^CC[ACGT]GG
1957 CCAGG
BstNI 692 CCTGG CC^[AT]GG
1957 CCAGG
DsaI 602 CCATGG C^C[GA][CT]GG
2132 CCATGG
EarI 171 GAAGAG CTCTTC(1/4)
1981 GAAGAG
EcoRII 692 CCTGG ^CC[AT]GG
1957 CCAGG
Ksp632I 171 GAAGAG CTCTTC(1/4)
1981 GAAGAG
MvaI 692 CCTGG CC^[AT]GG
1957 CCAGG
NcoI 602 CCATGG C^CATGG
2132 CCATGG
ScrFI 692 CCTGG CC^[ACGT]GG
1957 CCAGG
XmnI 385 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
462 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BfaI BpuAI BsaJI BsiYI
BslI BsmAI BstF5I BstYI Cfr13I
DdeI DpnII EcoT14I FokI HaeIII
HinfI HphI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I SfaNI StyI
TfiI Tru9I Tsp45I Tsp509I TspRI
XhoII