Restriction map for
pF1KB6940
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BcnI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 34 CCGC CCGC(-3/-1)
AcsI 207 GAATTT [GA]^AATT[CT]
Alw44I 538 GTGCAC G^TGCAC
AlwI 350 GGATC GGATC(4/5)
AlwNI 511 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 538 GTGCAC G^TGCAC
ApoI 207 GAATTT [GA]^AATT[CT]
Asp700I 46 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 538 GTGCAC G[AT]GC[AT]^C
AvaI 422 CTCGAG C^[CT]CG[GA]G
BanI 550 GGTGCC G^G[CT][GA]CC
BciVI 519 GGATAC GTATCC(6/5)
BclI 635 TGATCA T^GATCA
BglII 611 AGATCT A^GATCT
BmyI 538 GTGCAC G[AGT]GC[ACT]^C
BpmI 268 CTGGAG CTGGAG(16/14)
BsiHKAI 538 GTGCAC G[AT]GC[AT]^C
BsoBI 422 CTCGAG C^[CT]CG[GA]G
Bsp1286I 538 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 363 TGTACA T^GTACA
BspMI 343 GCAGGT ACCTGC(4/8)
BsrDI 681 CATTGC GCAATG(2/0)
BsrGI 363 TGTACA T^GTACA
BsrI 267 ACTGG ACTGG(1/-1)
BssKI 516 CCTGG ^CC[ACGT]GG
BstAPI 150 GCAGTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 71 GGATG GGATG(2/0)
BstNI 516 CCTGG CC^[AT]GG
BstYI 611 AGATCT [GA]^GATC[CT]
DsaI 143 CCATGG C^C[GA][CT]GG
EcoRII 516 CCTGG ^CC[AT]GG
FbaI 635 TGATCA T^GATCA
FokI 71 GGATG GGATG(9/13)
HindIII 496 AAGCTT A^AGCTT
MaeII 506 ACGT A^CGT
MaeIII 417 GTTAC ^GT[ACGT]AC
MflI 611 AGATCT [GA]^GATC[CT]
MvaI 516 CCTGG CC^[AT]GG
NcoI 143 CCATGG C^CATGG
NlaIV 550 GGTGCC GG[ACGT]^[ACGT]CC
PaeR7I 422 CTCGAG C^TCGAG
ScrFI 516 CCTGG CC^[ACGT]GG
SfaNI 585 GCATC GCATC(5/9)
SphI 36 GCATGC GCATG^C
SspBI 363 TGTACA T^GTACA
SspI 274 AATATT AAT^ATT
TspRI 203 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 422 CTCGAG C^TCGAG
XhoII 611 AGATCT [GA]^GATC[CT]
XmnI 46 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 124 GTAC GT^AC
364 GTAC
AflIII 237 ACATGT A^C[GA][CT]GT
569 ACATGT
AocI 283 CCTAAGG CC^T[ACGT]AGG
661 CCTGAGG
BseRI 475 CTCCTC GAGGAG(10/8)
664 GAGGAG
BsgI 148 GTGCAG GTGCAG(16/14)
508 GTGCAG
BspLU11I 237 ACATGT A^CATGT
569 ACATGT
Bsu36I 283 CCTAAGG CC^T[ACGT]AGG
661 CCTGAGG
Cac8I 36 GCATGC GC[ACGT]^[ACGT]GC
498 GCTTGC
Eco81I 283 CCTAAGG CC^T[ACGT]AGG
661 CCTGAGG
HaeIII 357 GGCC GG^CC
393 GGCC
HinfI 13 GACTC G^A[ACGT]TC
139 GACTC
HphI 427 GGTGA GGTGA(8/7)
465 GGTGA
MboII 46 GAAGA GAAGA(8/7)
334 GAAGA
MseI 154 TTAA T^TAA
278 TTAA
MwoI 150 GCAGTTAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
489 GCTGCCGAAGC
PleI 13 GACTC GAGTC(4/5)
139 GACTC
RsaI 124 GTAC GT^AC
364 GTAC
SmlI 422 CTCGAG C^T[CT][GA]AG
478 CTCAAG
TaqI 353 TCGA T^CGA
423 TCGA
Tru9I 154 TTAA T^TAA
278 TTAA
Tsp509I 208 AATT ^AATT
216 AATT
TthHB8I 353 TCGA T^CGA
423 TCGA
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BbvI BsaJI BsmAI DdeI
DpnII EcoT14I Fnu4HI ItaI MboI
MnlI NdeII NlaIII NspI Sau3AI
StyI TseI